In situ fabrication of cross-linked protein membranes by using microfluidics.

We report a novel technique for preparing cross-linked protein membranes within microchannels by using an interfacial cross-linking reaction. Glass microchannels with a Y input were assembled by using a simple adhesive bonding technique to achieve dual, parallel laminar flows. Membrane formation utilised an interfacial reaction at the liquid-liquid interface, which involved bovine serum albumin (aqueous solution with a flow rate of 300 microL min(-1)) and terephthaloyl chloride (xylene solution with a flow rate of 700 microL min(-1)), to form thin ( approximately 25 microm) cross-linked films along the length of the channel under the continuous pressure-driven laminar flow.

Development of water-conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales).

The thallus of the metzgerialean liverwort Symphyogyna brasiliensis Nets contains a strand of dead thick-walled tells with helicoidally-arranged pits that arc presumably involved in water transport. During the first phase of differentiation these cells undergo a 13-16-fold elongation while remaining thin-walled and almost unchanged in diameter. During subsequent maturation the walls become strongly thickened by deposition of highly electron-opaque material on extraplasmodesmal areas and of transparent material forming collars around plasmodesmata.