Pathogenic mechanisms and potential applications of extracellular vesicles from periodontal pathogens in periodontitis.

Periodontitis is a multifactorial disease characterized by chronic destruction of the periodontal supporting tissues and is closely associated with the dysbiosis of the plaque biofilm. It is the leading cause of tooth loss in adults. Bacterial extracellular vesicles (BEVs) are released from bacteria, which range in size from 20 to 400 nm.

The effect of different activation irrigations on intracanal smear layer removal: a vitro study.

Objective: To compare the effect of syringe irrigation technique, passive ultrasonic activation technique, EDDY activation technique and Er,Cr,YSGG laser activation technique on smear layer removal in root canals .

Methods: Forty mandibular first premolars with single canal were collected from patients in Qingdao Stomatological Hospital affiliated to Qingdao University. After root canal preparation with ProTaper Universal to F3, they were randomly divided into four groups (n = 10) according to different activation irrigations for the final washing: syringe irrigation (SI), passive ultrasonic activation (PU), EDDY activation (EDDY) and Er,Cr,YSGG laser activation (YSGG).

Improved mechanical performance and forming accuracy of ZrO fixed partial denture based on the digital light processing technology.

Fixed partial dentures are the primary treatment for dentition defects. Digital light processing (DLP) 3D printing technology is an advanced technique with significant advantages and potential in the field of dental restoration, particularly in cases requiring high precision and personalization. However, challenges persist in printing fixed partial dentures that meet the strength requirements for clinical applications.

GelMA hydrogels reinforced by PCL@GelMA nanofibers and bioactive glass induce bone regeneration in critical size cranial defects.

Background: The process of bone healing is complex and involves the participation of osteogenic stem cells, extracellular matrix, and angiogenesis. The advancement of bone regeneration materials provides a promising opportunity to tackle bone defects. This study introduces a composite hydrogel that can be injected and cured using UV light.

Three-dimensional structured PLCL/ADM bioactive aerogel for rapid repair of full-thickness skin defects.

The failure to treat deep skin wounds can result in significant complications, and the limitations of current clinical treatments highlight the pressing need for the development of new deep wound healing materials. In this study, a series of three-dimensional structured PLCL/ADM composite aerogels were fabricated by electrospinning and subsequently characterized for their microstructure, compression mechanics, exudate absorption, and hemostatic properties. Additionally, the growth of HSFs and HUVECs, which are involved in wound repair, was observed in the aerogels.

Caries Activity Tests and Associated Factors among 3-5-Year-Old Preschool Children in Zhao County, Hebei Province, China.

Aim: This study aimed to investigate the caries activity (CA) and its caries status in children aged 3-5 years in Zhao County, Hebei province, China and to explore the related factors.

Methods: Cariostat caries activity test and oral examination were performed on children aged 3-5 years old in Zhao county, Hebei province by a stratified sampling. A questionnaire survey was used to analyze the CA-related factors and the receiver operating characteristic curve (ROC) was to evaluate the diagnostic value of Cariostat caries activity test in children with early childhood caries (ECC).

[Investigation and analysis of oral health status of children with autism and their parents' cognition].

Purpose: To understand the cognitive level of parents of children with autism towards their oral health behaviors and oral health care, and to provide assistance in developing better oral health plans for children with autism.

Methods: Totally 117 children and parents with autism who visited our hospital from January to December 2018 were selected as the experimental group,while 121 non-autistic children during the same period were selected as the control group, the children's oral hygiene was checked. Questionnaire survey was conducted on the oral health behaviors of pediatric patients, including daily brushing frequency, use of toothpaste, assistance from parents in brushing teeth, and frequency of snacking.

Apoptotic vesicles from macrophages exacerbate periodontal bone resorption in periodontitis via delivering miR-143-3p targeting Igfbp5.

Abstrct: BACKGROUND: Apoptotic vesicles (ApoVs), which are extracellular vesicles released by apoptotic cells, have been reported to exhibit substantial therapeutic potential for inflammatory diseases and tissue regeneration. While extensive research has been dedicated to mesenchymal stem cells (MSCs), the investigation into immune cell-derived ApoVs remains limited, particularly regarding the function and fate of macrophage-derived ApoVs in the context of periodontitis (PD).

Results: Our study corroborates the occurrence and contribution of resident macrophage apoptosis in Porphyromonas gingivalis (Pg)-associated PD.

TEAD transcription factor family emerges as a promising therapeutic target for oral squamous cell carcinoma.

The treatment of oral squamous cell carcinoma (OSCC) remains a significant difficulty, as there has been no improvement in survival rates over the past fifty years. Hence, exploration and confirmation of new dependable treatment targets and biomarkers is imperative for OSCC therapy. TEAD transcription factors are crucial for integrating and coordinating multiple signaling pathways that are essential for embryonic development, organ formation, and tissue homeostasis.

Water-soluble polysaccharides extracted from Enteromorpha prolifera/PVA composite film functionalized as ε-polylysine with improved mechanical and antibacterial properties.

The issue of environmental protection has received sustained and widespread attention. In order to reduce environmental pollution related to traditional plastics, it is an incessant demand to design novel environment-friendly food packaging materials with excellent performance. Sulfated polysaccharide extracted from the "green tide" marine pollution Enteromorpha prolifera (SPE) has been innovatively transformed into a film-forming material for better utilization.

A transcriptomic analysis of dental pulp stem cell senescence in vitro.

Background/purpose: The use of human dental pulp stem cells (hDPSCs) as autologous stem cells for tissue repair and regenerative techniques is a significant area of global research. The objective of this study was to investigate the effects of long-term in vitro culture on the multidifferentiation potential of hDPSCs and the potential molecular mechanisms involved.

Materials And Methods: The tissue block method was used to extract hDPSCs from orthodontic-minus-extraction patients, which were then expanded and cultured in vitro for 12 generations.

[Caries experience and its correlation with caries activity of 4-year-old children in Miyun District of Beijing].

Objective: To investigate the prevalence of dental caries of 4-year-old children in Miyun District of Beijing by international caries detection and assessment system (ICDAS), to detect the caries activity Cariostat value and to analyze the correlation between the Cariostat value and dental caries.

Methods: Totally 815 children aged 4 years in 7 kindergartens in Miyun District of Beijing were recruited. The clinical examination of all children was conducted by one examiner using ICDAS.

[Recent evaluation and analysis of the clinical effect of two closed treatment methods in children with intracapsular condyle fracture].

To summarize and analyze clinical characteristics of pediatric mandibular condylar fractures and the long-term therapeutic effects of closed treatment. A retrospective study was conducted for pediatric condylar fracture in the Department of Oral and Maxillofacial Surgery of Peking University School and Hospital of Stomatology from October 2015 to October 2019, including 33 males (67.3%) and 16 females (32.

Analysis on the relationship between Schneiderian membrane thickening in the posterior maxillary region and periapical lesions by cone beam computed tomography.

Objectives: This study aimed to evaluate the thickening of sinus membrane, which is also named Schneiderian membrane (SM), in patients and its relationship with periapical lesions (PAL) in the posterior maxillary region to provide reference for the prevention and treatment of odontogenic maxillary sinusitis.

Methods: A retrospective analysis was conducted on 554 cone beam computed tomography (CBCT) imaging data of maxillary sinuses from 301 patients who met the inclusion criteria to determine the correlation between PAL and SM thickening in the posterior maxillary region. Cases of pathological SM were recorded and classified on the basis of the degree and type of SM thickening.

Digital robot-assisted minimally invasive impacted tooth extraction: A case report.

Objective: This study investigated the clinical effects and applicability of minimally invasive impacted teeth extraction using digital robots.

Methods: A marker was bonded to the non-surgical area before surgery. A Cone-Beam Computed Tomography (CBCT) scan was obtained and uploaded to the robot software to determine the drilling position of the ring drill.

Injectable hybrid nanofibrous spheres made of PLA and nano-hydroxyapatite for cell delivery and osteogenic induction.

Introduction: Nanofibrous spheres, with their injectable format and biomimetic three-dimensional topologies that emulate the complexity of natural extracellular environments, have become increasingly attractive for applications in biomedical and regenerative medicine. Our research contributes to this growing field by detailing the design and fabrication of a novel series of polylactic acid/nano-hydroxyapatite (PLA/nHA) hybrid nanofibrous spheres.

Methods: These advanced structures were created by integrating electrospinning and electrospray techniques, which allowed for precise control over the nanofibrous spheres, especially in size.

Injectable GelMA Hydrogel Microspheres with Sustained Release of Platelet-Rich Plasma for the Treatment of Thin Endometrium.

Platelet-rich plasma (PRP) intrauterine infusion has been demonstrated to be effective in treating thin endometrium and achieving pregnancy. However, the rapid release of growth factors limits its effectiveness in clinical applications, and thus, multiple intrauterine infusions are often required to achieve therapeutic efficacy. In this study, a GelMA hydrogel microsphere biomaterial is developed using droplet microfluidics to modify the delivery mode of PRP and thus prolong its duration of action.