Enhancing astaxanthin accumulation in immobilized Haematococcus pluvialis via alginate hydrogel membrane.

Immobilized cultivation is anticipated to be effective for enhancing both biomass and astaxanthin accumulation in Haematococcus pluvialis (H. pluvialis). A novel fabrication method of alginate hydrogel membrane (AHM) was introduced for immobilized cultivation of H.

WTAP, a conserved mA writer, can promote the antiviral immunity of Miichthysmiiuy.

N6-methyladenosine (mA) is one of the most prevalent modifications found in eukaryotic mRNA and has been implicated in the regulation of cell proliferation, development, invasion, apoptosis, and immunity. In this study, we first conducted a structural and evolutionary analysis of Wilms' tumour 1-associating protein (WTAP) in vertebrates, and the results showed that WTAP in vertebrates is conserved particularly in mammals and fish. We subsequently investigated the involvement of WTAP in the antiviral immune response of fish and discovered that the expression of Miichthys miiuy (mmiWTAP) decreased in response to stimulation with Siniperca chuatsi rhabdovirus (SCRV) and poly(I:C).

Distributions of DMS and DMSP and the influences of planktonic community assemblages in the Bohai Sea and Yellow Sea.

Dimethyl sulfide (DMS) and dimethylsulfoniopropionate (DMSP) are important sulfur compounds influenced by community assemblages of plankton. The distributions of DMS, DMSP, DMSP lyase activity (DLA), DMSP-consuming bacteria (DCB), and community structures of phytoplankton and zooplankton were investigated during summer in the Bohai Sea and Yellow Sea. The variety ranges of DMS, dissolved DMSP (DMSP), and particulate DMSP (DMSP) concentrations in the surface seawater were 1.

The carbon transport mediated by the mild oxygen minimum zone (OMZ) in the seamount area of the Western Pacific.

This study analyzed the relationship between the oxygen minimum zone (OMZ) and various types of carbon, such as POC, DOC, and DIC, in the Y3 seamount area in the Western Pacific. The results indicated that the OMZ was located at 200-1000 m and a threshold of 100 μmol/kg was established for this area of the Western Pacific. The DOC and POC changed drastically out of OMZ while they were relatively stable within the OMZ due to the low oxygen.

Spatial distribution of bacteria in response to phytoplankton community and multiple environmental factors in surface waters in Sanggou Bay.

Coastal bays link terrestrial and oceanic carbon reservoirs and play important roles in marine carbon cycles. Particulate organic carbon (POC) produced by phytoplankton is a major autochthonous carbon source in coastal bays. Previous studies on the fate of POC produced by phytoplankton mainly focused on the relationship between phytoplankton and zooplankton in classic food webs, while our knowledge on the roles of bacterioplankton is still limited, particularly in bays under highly intensive aquaculture activities.

The metabolomics provides insights into the Pacific abalone () response to low temperature stress.

The low temperatures in winter, particularly the cold spells in recent years, have posed significant threats to China's abalone aquaculture industry. The low temperature tolerance of cultured abalone has drawn plenty of attention, but the metabolic response of abalone to low-temperature stress remains unclear. In this study, we investigated the metabolomic analysis of Pacific abalone () during low-temperature stress.

Genotype-by-Environment Effects of Cis-Variations in the Atgl Promoter Mediate the Divergent Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species.

Phenotypic plasticity plays an essential role in adaptive evolution. However, the molecular mechanisms of how genotype-by-environment interaction (G × E) effects shape phenotypic plasticity in marine organisms remain poorly understood. The crucial temperature-responsive trait triacylglycerol (TAG) content and its major gene adipose triglyceride lipase (Atgl) expression have divergent plastic patterns in two congeneric oyster species (Crassostrea gigas and Crassostrea angulata) to adapt to relative-cold/northern and relative-warm/southern habitats, respectively.

Sugar Auxiliary Group Assisted Diversity-Oriented Enzymatic Modular Synthesis of 0-Series Ganglioside Glycans.

Owing to the inaccessibility of β1-4-N-acetylgalactosaminyltransferase for direct glycan chain elongation, the enzymatic synthesis of 0-series gangliosides with extended backbones has not been explored. In this study, sialic acid was enzymatically introduced as an auxiliary group to overcome the limitation of substrate specificity of Campylobacter jejuni β1-4-N-acetylgalactosaminyltransferase (CjCgtA) to achieve the synthesis of desired extended 0-series ganglioside core structures, and the sialic acid auxiliary group could be removed by sialidase at appropriate stages. A bacterial α2-6-sialyltransferase from Photobacterium damselae (Pd2,6ST) exhibited unexpected acceptor substrate specificity for 0-series ganglioside core structures, providing ready access to complex gangliosides bearing the sialyl N-acetylgalactosamine unit.

Inferring gene-pathway associations from consolidated transcriptome datasets: an interactive gene network explorer for .

Although an established model organism, remains comparatively inaccessible to high throughput screens, and alternative bioinformatic approaches still rely on unconnected datasets and outdated algorithms. Here, we report a new approach to consolidating RNA-seq and microarray data based on a systematic exploration of parameters and computational controls, enabling us to infer functional gene associations from their co-expression patterns. To illustrate the power of this approach, we took advantage of new data regarding a previously studied pathway, the biogenesis of a secretory organelle called the mucocyst.

Protein degradation mechanisms during refrigerated storage of gazami crab (Portunus trituberculatus) at endogenous and microbial-derived enzyme levels.

During storage, the proteins of gazami crab (Portunus trituberculatus) are prone to hydrolysis into amino acids and biogenic amines, in which enzymes play a critical role. However, studies exploring spoilage mechanisms from the perspective of enzymes are limited. This study identified 84 endogenous and 52 microbial-derived proteolytic enzymes and peptidases by proteomics and metagenomics.

A comprehensive RNA virome identified in the oyster Magallana gigas reveals the intricate network of virus sharing between seawater and mollusks.

Background: As a globally farmed oyster species, Magallana gigas has garnered significant attention due to the contaminated RNA viruses that have caused illness in humans. However, limited knowledge is available on the bioaccumulation status and overall diversity of RNA virome in the M. gigas digestive tissues (DTs).

Rapid Sex Identification in Spotted Knifejaw (Oplegnathus punctatus) Using tmem88 Gene Structural Variation Markers.

Spotted knifejaw (Oplegnathus punctatus) is an economically important marine cultured species exhibiting a unique complex sex chromosome system (XXXX in females and XXY in males), with males possessing one fewer chromosome (2n = 47) than females (2n = 48) and an abnormally large Y chromosome. Additionally, males demonstrate significant growth advantages over females. Rapid and accurate sex identification is essential for effective culture management, selective breeding, and population control.

A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation.

The CoA thioester of 2-(carboxymethyl)cyclohexane-1-carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate-reducing culture N47. This study identified and characterised two acyl-CoA dehydrogenases (ThnO/ThnT) and an intramolecular CoA-transferase (ThnP) encoded within the substrate-induced thn operon, which contains genes for anaerobic degradation of naphthalene. ThnP is a CoA transferase belonging to the family I (Cat 1 subgroup) that catalyses the intramolecular CoA transfer from the carboxyl group of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA to its carboxymethyl moiety, forming 2-carboxycyclohexylacetyl-CoA.

Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.).

The sea toad genus Chaunax is a group of small benthic fishes that predominantly inhabiting the deep seas of the Atlantic, Indian, and Pacific Oceans. Although they have the potential to make excellent systems for studies of evolutionary adaptation to deep-sea environments, genomic research on Chaunax has been hindered by a scarcity of high-quality genomic resources. We present a chromosome-scale genome assembly of a Chaunax specimen generated using PacBio long-read sequencing and high-throughput chromosome conformation capture technology.

Effect of liquid nitrogen freezing pretreatment on the meat quality of gazami crab (Portunus trituberculatus) during frozen storage.

Liquid nitrogen freezing (LNF) pretreatment is an effective means of maintaining seafood quality during frozen storage. To improve the meat quality of gazami crab (Portunus trituberculatus) during frozen storage, this study investigated the effects of LNF temperatures on the meat quality of gazami crab. Fresh crab was pre-treated with different LNF temperatures (-60 °C, -80 °C, -100 °C, -120 °C), and then freeze in refrigerator at -18 °C.

Implications of seawater characteristics on dissolved heavy metals in near-shore surface waters of the Yellow Sea.

To explore the response of dissolved heavy metals (HMs) along the Yellow Sea coast to seawater characteristics, we assessed the temporal and spatial variations as well as pollution levels of six dissolved HMs (Cu, Zn, Pb, Cd, Hg, and As) in surface seawater from 2021 to 2023. The results indicated that the average concentrations of HMs in the seawater were ranked as follows: Zn > Cu > As > Pb > Cd > Hg, with specific concentration ranges of 4.70-7.

Pyruvate Abundance Confounds Aminoglycoside Killing of Multidrug-Resistant Bacteria via Glutathione Metabolism.

To explore whether the metabolic state reprogramming approach may be used to explore previously unknown metabolic pathways that contribute to antibiotic resistance, especially those that have been neglected in previous studies, pyruvate reprogramming was performed to reverse the resistance of multidrug-resistant . Surprisingly, we identified a pyruvate-regulated glutathione system that occurs by boosting glycine, serine, and threonine metabolism. Moreover, cysteine and methionine metabolism played a key role in this reversal.

Telomere-to-telomere gapless genome assembly of the giant grouper (Epinephelus lanceolatus).

The giant grouper (Epinephelus lanceolatus) is a large coral reef fish distributed in the Indian Ocean and the Pacific Ocean. With a high market value, this species can grow up to approximately 2.7 meters in length and weigh 440 kilograms.

Characterization and functional analysis of a novel goose-type lysozyme from teleost Sebastes schlegelii with implications for antibacterial defense and immune cell modulation.

Lysozymes are crucial enzymes involved in the innate immune response against bacterial pathogens. In this study, we identified and characterized a goose-type lysozyme gene (SsLyG) from the black rockfish Sebastes schlegelii, an economically important aquaculture species. The deduced amino acid sequence of SsLyG contains 495 residues, which inculded a signal peptide, an immunoglobulin domain, and a goose egg-white lysozyme (GEWL) domain.

Effects of mitochondrial lipidome alterations on quality deterioration of Larimichthys crocea postmortem storage: New insight from the perspective of mediating mitochondria-dependent apoptosis.

Apoptosis occurs in the myocyte of fish postmortem storage. Based on the important role of mitochondrial lipid molecules in regulating apoptosis, the study aims to investigate the potential impact of mitochondrial lipids on apoptosis and quality deterioration of large yellow croaker. A total of 1079 lipid molecule species in 13 classes were identified in mitochondria.

Multiscale and stimuli-responsive biosensing in biomedical applications: Emerging biomaterials based on aggregation-induced emission luminogens.

Biosensors play a critical role in the diagnosis, treatment, and prognosis of diseases, with diverse applications ranging from molecular diagnostics to in vivo imaging. Conventional fluorescence-based biosensors, however, often suffer from aggregation-caused emission quenching (ACQ), limiting their effectiveness in high concentrations and complex environments. In contrast, the phenomenon of aggregation-induced emission (AIE) has emerged as a promising alternative, where luminescent materials exhibit strong emission in the aggregated state with good photostability, biocompatibility, large Stokes shift, high quantum yield, and tunable emission.

Superhydrophobic Surfaces as a Potential Skin Coating to Prevent Jellyfish Stings: Inhibition and Anti-Tentacle Adhesion in Nematocysts of Jellyfish .

The development of skin-protective materials that prevent the adhesion of cnidarian nematocysts and enhance the mechanical strength of these materials is crucial for addressing the issue of jellyfish stings. This study aimed to construct superhydrophobic nanomaterials capable of creating a surface that inhibits nematocyst adhesion, therefore preventing jellyfish stings. We investigated wettability and nematocyst adhesion on four different surfaces: gelatin, polydimethylsiloxane (PDMS), dodecyl trichlorosilane (DTS)-modified SiO, and perfluorooctane triethoxysilane (PFOTS)-modified TiO.

Cold-blooded vertebrate utilizes behavioral fever to alleviate T cell apoptosis and optimize antimicrobial immunity.

Fever confers significant survival benefits on endotherms by optimizing both innate and adaptive immunity. Ectotherms achieve thermoregulation using behavioral strategies, but existing evidence supports its enhancement effect on innate immunity only. Therefore, it remains unknown whether the coordination between fever and adaptive immunity was independently acquired by endotherms or instead represents a gradually evolved function common to vertebrates.

Sulfur metabolism and response to light in Ulva prolifera green tides.

The outbreak of Ulva prolifera blooms causes significant changes in the coastal sulfur cycle due to the high production of dimethylsulfoniopropionate (DMSP) and the emission of dimethylsulfide (DMS). However, the sulfur metabolism mechanism of U. prolifera has not been thoroughly investigated.

Insight into the mechanism of Maillard reaction and lipids mutually contribute to the flavor release of squid fillets during the drying process.

Dried squid fillet is a popular seafood product with a unique flavor. However, its flavor release mechanism is unclear. In this study, volatile compounds (VOCs) were dynamically monitored in thawed squid (TS), salted squid (SS) and dried squid for 6 h (D6) and 24 h (D24).