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Article Synopsis
  • - Gold nanoparticles (AuNPs) create a protein corona that can alter the structure and identification of proteins, making it essential to understand how to analyze these coronas effectively.
  • - This study focuses on the dissociation of proteins from the corona, identifying temperature and cysteine as key factors that affect this process over time.
  • - Findings indicate that the timing of Au-S bond formation is crucial for protein dissociation and enrichment, with significant changes in the secondary structure of beta-lactoglobulin (βlg) observed after certain analytical methods were applied.
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