Metabolic engineering of Escherichia coli for de novo production of 5-hydroxyvalerate via L-lysine α-oxidase pathway.

5-hydroxyvalerate (5-HV) is a crucial C5 platform chemical with versatile applications, yet its efficient production remains a challenge. The Raip, gabT, and yahK genes were integrated into the E. coli LE genome, deleted gabD, and enhanced gabP expression, resulting in the QluMG strain.

A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose.

D-Allose is a rare cis-caprose with a wide range of physiological functions, which has a wide range of applications in medicine, food, and other industries. L-Rhamnose isomerase (L-Rhi) is the earliest enzyme found to catalyze the production of D-allose from D-psicose. This catalyst has a high conversion rate, but its specificity for substrates is limited; thus, it cannot fulfill the requirements of industrial production of D-allose.

Construction of a double heterojunction between graphite carbon nitride and anatase TiO with co-exposed (101) and (001) faces for enhanced photocatalytic degradation.

This study aimed to promote the separation of photogenerated carriers and improve the redox performance of graphite carbon nitride (g-CN) by synthesizing a double-heterojunction-structure photocatalyst, g-CN/(101)-(001)-TiO, through the solvothermal method. The photocatalyst comprised a Z-system formed from g-CN and the (101) plane of TiO, as well as a surface heterojunction formed from the (101) and (001) planes of TiO. The results showed that g-CN/(101)-(001)-TiO had strong photocatalytic activity and stable performance in the photodegradation of paracetamol.

A mechanistic study on electro-Fenton system cooperating with phangerochate chrysosporium to degrade lignin.

The combined catalytic system of Electro-Fenton (E-Fenton) and () was constructed in liquid medium with additional potential to overcome the limitations of lignin degradation by white rot fungi alone. To further understand the mechanism of synergistic catalysis, we optimized the optimum potential for lignin catalysis by and built synergistic separate catalyses. After 48 h of incubation, the optimum growth environment and the highest lignin degradation rate (43.

Methane production from wheat straw pretreated with CaO/cellulase.

There are various lignocellulosic biomass pretreatments that act as attractive strategies to improve anaerobic digestion for methane (CH) generation. This study proposes an effective technique to obtain more CH the hydrothermal coupled calcium peroxide (CaO) co-cellulase pretreatment of lignocellulosic biomass. The total organic carbon in the hydrolysate of samples treated with 6% CaO and 15 mg enzyme per g-cellulose was 7330 mg L, which represented an increase of 92.

Highly sensitive and selective detection of PCB 77 using an aptamer-catalytic hairpin assembly in an aquatic environment.

Polychlorinated biphenyls (PCBs) are synthetic organic compounds that are extremely difficult to break down in water and can accumulate in human fat and organisms. However, methods that can be used to detect large amounts of PCBs remain unsatisfactory, as they are generally overly sensitive and involve complex operations. An aptamer-based catalytic hairpin assembly (aptamer-CHA) reaction for the selective detection of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) was developed.

Engineered Microstructure Derived Hierarchical Deformation of Flexible Pressure Sensor Induces a Supersensitive Piezoresistive Property in Broad Pressure Range.

Fabricating flexible pressure sensors with high sensitivity in a broad pressure range is still a challenge. Herein, a flexible pressure sensor with engineered microstructures on polydimethylsiloxane (PDMS) film is designed. The high performance of the sensor derives from its unique pyramid-wall-grid microstructure (PWGM).

Synergistic anticancer effect of Grb2 and ITGA1 on cancer cells highly expressing Grb2 through suppressing ERK phosphorylation.

Background: Growth factor receptor bound protein 2 (Grb2) is known to be an adaptor protein that provides a critical link between cell surface growth factor receptors and the MAPK signaling. It was shown that high expression of Grb2 enhances cancer cells proliferation, invasion and malignant transformation.

Objective: In this study, we aimed to systemically understand the function of Grb2 in cancer.

Preparation and photocatalytic performance of tungstovanadophosphoric heteropoly acid salts.

Tungstovanadophosphoric heteropoly acid HPWVO·5.76HO (HPWV) has been synthesized stepwise acidification and gradual addition of elements. Some metals like Fe, Al and Cu were introduced into the heteropoly acid (HPA) in the molar ratio of 10 : 6, 10 : 6 and 10 : 4 respectively.