1 results match your criteria: "Qian-jiang College of Hangzhou Normal University[Affiliation]"
[Prokaryotic expression of trigeminy artificial fusion gene of Leptospira interrogans and the immunogenicity of its products].
Zhejiang Da Xue Xue Bao Yi Xue Ban
November 2008
Faculty of Basic Medicine, Qian-jiang College of Hangzhou Normal University, Hangzhou 310013, China.
Objective: To construct lipL32/1-lipL21-OmpL1/2 fusion gene of Leptospira interrogans and its prokaryotic expression system, and to identify the immunogenicity of its products.
Methods: PCR using linking primers was applied to construct lipL32/1-lipL21-OmpL1/2 fusion gene and a prokaryotic expression system of the fusion gene was then established using routine genetic engineering technique. SDS-PAGE was used to examine output of the target recombinant protein rLipL32/1-LipL21-OmpL1/2.