Efficient antigen cross-presentation through coating conventional aluminum adjuvant particles with PEI.

Classical aluminum adjuvant is a deficient antigen carrier for cross-presentation and cross-priming of CD8 cytotoxic T cells. Our previous research has demonstrated that cross-presentation efficiency significantly increased when antigens are conjugated covalently to α-AlO nanoparticles. Here we found that coating conventional aluminum adjuvants with polyethyleneimine (PEI) could enhance antigen cross-presentation of DCs (dendritic cells) in vitro and in vivo.