Immunohistochemical detection of O-acetylated sialomucins in intestinal metaplasia and carcinoma of the stomach.

Eighty-two selected gastric mucosal biopsy or resection specimens were stained both conventionally, to classify subtypes of intestinal metaplasia and carcinoma, and immunohistochemically with a mouse monoclonal antibody (MMM-17), raised against normal human colonic mucin, which has an affinity for di- and/or tri-O-acetylated sialomucin. The aims of the study were to reassess the prevalence of O-acetylated sialomucins in normal, metaplastic and carcinomatous gastric mucosa and to investigate whether the production of these mucins by intestinal metaplasia is related to its associated mucosal pathology. O-acetylated sialomucins were not seen in normal mucosa.

Malignant hyperthermia during isoflurane anaesthesia.

Two cases are presented which illustrate the essential features, diagnosis and management of malignant hyperthermia. Both cases occurred in association with isoflurane, and in patients who were exposed during previous anaesthetics to recognised trigger agents without apparent manifestation of the syndrome.

C-reactive protein in acute renal failure.

This paper demonstrates the utility of C-reactive protein (CRP) in the diagnosis of infection in patients with acute renal failure. C-reactive protein can be assayed using plasma as effectively as using serum, thus avoiding the problems of microclots in serum, which can occur in samples from a heparinised patient. Plasma concentrations of C-reactive protein are unaffected by the process of haemodialysis.