Formation of singlet oxygen during farmorubicin oxidation.

The enhanced generation of singlet oxygen (1O2) during oxidation of farmorubicin in the Co(II) + H2O2 system was studied using chemiluminescent, fluorescent and spectrophotometic techniques. The influence of 1O2-quenchers, catalase, superoxide anion radical (O2*-) and hydroxyl radical (HO*) scavengers on the light emission was studied. The spectrophotometric determination of 1O2 was based on bleaching of p-nitrosodimethylaniline caused by an intermediate product of the reaction of 1O2 with imidazole, and was followed by monitoring the decrease in optical density at 440 nm.

Luminescence investigations of redox cycling of adriamycin.

The light emission from the adriamycin + Co2+ + H2O2 system has been studied. Chemiluminescence, fluorescence and absorption spectra were measured. The fluorescence spectra were time-dependent exhibiting maxima at 555, 590 and 645 nm.