1 results match your criteria: "Poland. Electronic address: tarasiuk@univ.szczecin.pl.[Affiliation]"
DNA damage induced by NADPH cytochrome P450 reductase-activated idarubicin in sensitive and multidrug resistant MCF7 breast cancer cells.
Pharmacol Rep
February 2017
Department of Biochemistry, Faculty of Biology, University of Szczecin, 3c Felczaka St., 71-412 Szczecin, Poland; Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, 71-415 Szczecin, 13 Wąska St., Poland. Electronic address:
Background: Idarubicin (IDA) is one of clinically important anticancer drugs belonging to the anthracycline antibiotic family. The aim of this study was to examine DNA damage induced by NADPH cytochrome P450 reductase (CPR)-activated IDA in human sensitive MCF7 and multidrug resistant MCF7/DOX (overexpressing P-gp) breast adenocarcinoma cells.
Methods: The evaluation of DNA fragmentation caused by single strand breaks (SSB) and double strand breaks (DSB) was performed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) test.