Genome-based development and clinical evaluation of a customized LAMP panel to rapidly detect, quantify, and determine antibiotic sensitivity of Escherichia coli in native urine samples from urological patients.

Purpose: We designed and tested a point of care test panel to detect E.coli and antibiotic susceptibility in urine samples from patients at the point of care in the urological department. The aim of this approach is to facilitate choosing an appropriate antibiotic for urinary tract infections (UTI) at first presentation in the context of increasing antibiotic resistance in uropathogens worldwide.

Forearm elevation impairs local static handgrip endurance likely through reduction in vascular conductance and perfusion pressure: revisiting Rohmert's curve.

Maximal isometric contraction time (MICT) is critical for most motor tasks and depends on skeletal muscle blood flow at < 40% of maximal voluntary strength (MVC). Whether limb work positions associated with reduced perfusion pressure and facilitated vessel compression affect MICT is largely unknown. In 14 healthy young men we therefore assessed bilateral handgrip MICT at 15, 20, 30, 40, and 70% of MVC in horizontal forearm positions of 0.

Coordination-induced axial chirality controls the metal-centred configuration in a stereogenic-at-iron catalyst.

A new approach is introduced to control the metal-centred configuration of stereogenic-at-iron catalysts by utilizing axial ligand chirality, which becomes locked upon metal coordination. This strategy is applied to an iron catalyst containing two chelating -(2-pyridyl)-substituted triazol-5-ylidene mesoionic carbenes (MICs) resulting in a helical topology with a stereogenic iron centre.

Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations.

The mutational landscape of TP53, a tumor suppressor mutated in about half of all cancers, includes over 2,000 known missense mutations. To fully leverage TP53 mutation status for personalized medicine, a thorough understanding of the functional diversity of these mutations is essential. We conducted a deep mutational scan using saturation genome editing with CRISPR-mediated homology-directed repair to engineer 9,225 TP53 variants in cancer cells.

Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts.

The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation and co-cultured it with KFs and NFs. We detected its effect on cell proliferation using the CCK-8 assay; cell migration ability by cell scratch and Transwell assays; extracellular matrix synthesis using the hydroxyproline content assay; the expression levels of genes associated with fibrosis by PCR assay; and the expression levels of proteins related to fibrosis in the cells using the Western Blot method.

Influence of SARS-CoV-2 variants of concern and maternal vaccination status on neonatal outcome.

SARS-CoV-2 infection during pregnancy can adversely affect maternal and neonatal health, although risks vary depending on the variant of concern (VOC). Omicron, although highly infectious, causes fewer maternal and neonatal complications than earlier VOC, so vaccination may be considered unnecessary in planned pregnancy. Using data from the CRONOS registry, we compared pregnancy outcomes according to VOC and vaccination status.

Clinical validation of a wireless patch-based polysomnography system.

Study Objectives: Onera Health has developed the first wireless, patch-based, type-II PSG system, the Onera Sleep Test System (STS), to allow studies to be performed unattended at the patient's home or in any bed at a medical facility. The goal of this multicenter study was to validate data collected from the patch-based PSG to a traditional PSG for sleep staging and AHI.

Methods: Simultaneous traditional PSG and patch-based PSG study data were obtained in a sleep laboratory from 206 participants with a suspected sleep disorder recruited from 7 clinical sites.

Characterizing of dropouts in the mental health of refugees and asylum seekers (MEHIRA) study examining the effects of a stepped and collaborative care model - a multicentered rater-blinded randomized controlled trial.

Background: Dropout from healthcare interventions can negatively affect patients and healthcare providers through impaired trust in the healthcare system and ineffective use of resources. Research on this topic is still largely missing on refugees and asylum seekers. The current study aimed to characterize predictors for dropout in the Mental Health in Refugees and Asylum Seekers (MEHIRA) study, one of the largest multicentered controlled trials investigating the effectiveness and cost-effectiveness of a nationwide stepped and collaborative care model.

Spectroscopy and Bonding Analysis of ArBO ( = 1-3) Cations That Possess Argon-Boron Multiple Bonds.

ArBO ( = 1-3) complexes have been prepared and subjected to spectroscopic characterization in the gas phase. Mass-selected infrared photodissociation spectroscopy, in combination with theoretical calculations, reveals the coexistence of two nearly isoenergetic structural isomers in ArBO. One isomer entails two equivalent Ar atoms chemically bound to BO, while the other features an ArBO core ion accompanied by a weakly tagging argon atom.

Rapid Development of Modified Vaccinia Virus Ankara (MVA)-Based Vaccine Candidates Against Marburg Virus Suitable for Clinical Use in Humans.

Background/objectives: Marburg virus (MARV) is the etiological agent of Marburg Virus Disease (MVD), a rare but severe hemorrhagic fever disease with high case fatality rates in humans. Smaller outbreaks have frequently been reported in countries in Africa over the last few years, and confirmed human cases outside Africa are, so far, exclusively imported by returning travelers. Over the previous years, MARV has also spread to non-endemic African countries, demonstrating its potential to cause epidemics.

Extracellular Vesicles and PlantCrystals for Improved Bioavailability of Curcumin as a BCS Class IV Drug.

The limited water solubility of active compounds remains a significant challenge for efficient dermal drug delivery, particularly for BCS class IV drugs such as curcumin. This study aimed to enhance curcumin's dermal penetration using two strategies: extracellular vesicles (EVs) and plantCrystals derived from soybeans. EVs were isolated using classical methods.

Altered Protein Kinase A-Dependent Phosphorylation of Cav1.2 in Left Ventricular Myocardium from Haploinsufficient Rat Hearts.

encodes the α1c subunit of the L-type Ca channel, Cav1.2. Ventricular myocytes from haploinsufficient () rats exhibited reduced expression of Cav1.

PAC1 Agonist Maxadilan Reduces Atherosclerotic Lesions in Hypercholesterolemic ApoE-Deficient Mice.

A possible involvement of immune- and vasoregulatory PACAP signaling at the PAC1 receptor in atherogenesis and plaque-associated vascular inflammation has been suggested. Therefore, we tested the PAC1 receptor agonist Maxadilan and the PAC1 selective antagonist M65 on plaque development and lumen stenosis in the ApoE atherosclerosis model for possible effects on atherogenesis. Adult male ApoE mice were fed a cholesterol-enriched diet (CED) or standard chow (SC) treated with Maxadilan, M65 or Sham.

The Diagnostic Accuracy of an Electrocardiogram in Pulmonary Hypertension and the Role of "R V1, V2 + S I, aVL - S V1".

: Pulmonary hypertension (PH) can cause characteristic electrocardiographic (ECG) changes due to right ventricular hypertrophy and/or strain. The aims of the present study were to explore the diagnostic accuracy of ECG parameters for the diagnosis of PH, applying the recently adjusted mean pulmonary artery pressure (mPAP) threshold of >20 mmHg, and to determine the role of "R V1, V2 + S I, aVL - S V1". : Between July 2012 and November 2023, 100 patients without PH, with pulmonary arterial hypertension, or with chronic thromboembolic pulmonary hypertension were retrospectively enrolled.

Charge Movements and Conformational Changes: Biophysical Properties and Physiology of Voltage-Dependent GPCRs.

G protein-coupled receptors (GPCRs) regulate multiple cellular functions and represent important drug targets. More than 20 years ago, it was noted that GPCR activation (agonist binding) and signaling (G protein activation) are dependent on the membrane potential (V). While it is now proven that many GPCRs display an intrinsic voltage dependence, the molecular mechanisms of how GPCRs sense depolarization of the plasma membrane are less well defined.

Determinants of Overall and Readmission-Free Survival in Patients with Metastatic Epidural Spinal Cord Compression.

Background: The aim of this study was to assess the surgical outcomes and survival of patients surgically treated for metastatic epidural spinal cord compression (MESCC), with a specific focus on identifying factors that influence overall survival and readmission-free survival.

Methods: All patients who underwent surgery for spine metastases at our department in the period 2018-2022 were included in the study.

Results: A total of 175 patients (n = 71 females, median age 67.

Metabolites in the Dance: Deciphering Gut-Microbiota-Mediated Metabolic Reprogramming of the Breast Tumor Microenvironment.

Breast cancer (BC), a major cause of death among women worldwide, has traditionally been linked to genetic and environmental factors. However, emerging research highlights the gut microbiome's significant role in shaping BC development, progression, and treatment outcomes. This review explores the intricate relationship between the gut microbiota and the breast tumor microenvironment, emphasizing how these microbes influence immune responses, inflammation, and metabolic pathways.

The impact of white matter lesions and subclinical cerebral ischemia on postoperative cognitive training outcomes after heart valve surgery: A randomized clinical trial.

Background: White matter lesions and subclinical cerebral ischemia (SCI) are described as risk factors for postoperative cognitive decline (POCD) following cardiac surgery. This report aims to investigate the effect of brain lesions on postoperative cognitive training outcomes.

Methods: In a randomized, treatment-as-usual controlled trial, elderly patients scheduled for elective heart valve surgery participated.

Gestational Duration and Postnatal Age-Related Changes in Aperiodic and Periodic Parameters in Neonatal and Toddler Electroencephalogram (EEG).

The brain develops most rapidly during pregnancy and early neonatal months. While prior electrophysiological studies have shown that aperiodic brain activity undergoes changes across infancy to adulthood, the role of gestational duration in aperiodic and periodic activity remains unknown. In this study, we aimed to bridge this gap by examining the associations between gestational duration and aperiodic and periodic activity in the EEG power spectrum in both neonates and toddlers.

Psychological factors associated with Long COVID: a systematic review and meta-analysis.

Background: Despite the immense impact of Long COVID on public health and those affected, its aetiology remains poorly understood. Findings suggest that psychological factors such as depression contribute to symptom persistence alongside pathophysiological mechanisms, but knowledge of their relative importance is limited. This study aimed to synthesise the current evidence on psychological factors potentially associated with Long COVID and condition-relevant outcomes like quality of life.

To intubate or to resuscitate: the effect of simulation-based training on advanced airway management during simulated paediatric resuscitations.

Background: We aimed to measure the effect of a 2-day structured paediatric simulation-based training (SBT) on basic and advanced airway management during simulated paediatric resuscitations.

Methods: Standardised paediatric high-fidelity SBT was conducted in 12 of the 15 children's hospitals in Hesse, Germany. Before and after the SBT the study participants took part in two study scenarios (PRE and POST scenario), which were recorded using an audio-video system.

Quantitative comparison of a mobile, tablet-based eye-tracker and two stationary, video-based eye-trackers.

The analysis of eye movements is a noninvasive, reliable and fast method to detect and quantify brain (dys)function. Here, we investigated the performance of two novel eye-trackers-the Thomas Oculus Motus-research mobile (TOM-rm) and the TOM-research stationary (TOM-rs)-and compared them with the performance of a well-established video-based eye-tracker, i.e.

Modeling dependent group judgments: A computational model of sequential collaboration.

Sequential collaboration describes the incremental process of contributing to online collaborative projects such as Wikipedia and OpenStreetMap. After a first contributor creates an initial entry, subsequent contributors create a sequential chain by deciding whether to adjust or maintain the latest entry which is updated if they decide to make changes. Sequential collaboration has recently been examined as a method for eliciting numerical group judgments.