4 results match your criteria: "Pharmanex Beijing Clinical Pharmacology Center[Affiliation]"
Altered proteomic polymorphisms in the caterpillar body and stroma of natural Cordyceps sinensis during maturation.
PLoS One
June 2015
Department of Applied Biology and Chemistry Technology, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong; Shenzhen TCM Pharmacy and Molecular Pharmacology Kay Laboratory, Hong Kong Polytechnic University, Shenzhen, Guangdong, China; NS Center for Anti-Aging Research, Provo, UT, United States of America.
Objective: To examine the maturational changes in proteomic polymorphisms resulting from differential expression by multiple intrinsic fungi in the caterpillar body and stroma of natural Cordyceps sinensis (Cs), an integrated micro-ecosystem.
Methods: The surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) biochip technique was used to profile the altered protein compositions in the caterpillar body and stroma of Cs during its maturation. The MS chromatograms were analyzed using density-weighted algorithms to examine the similarities and cluster relationships among the proteomic polymorphisms of the Cs compartments and the mycelial products Hirsutella sinensis (Hs) and Paecilomyces hepiali (Ph).
[Amplicon density-weighted algorithms for analyzing dissimilarity and dynamic alterations of RAPD polymorphisms of Cordyceps sinensis].
Beijing Da Xue Xue Bao Yi Xue Ban
August 2014
Department of Applied Biology and Chemistry Technology, Hong Kong Polytechnic University, Hong Kong; NS Center for Anti-Aging Research, Provo, UT 84601, USA.
Objective: To examine the dynamic maturational alterations of random amplified polymorphic DNA (RAPD) molecular marker polymorphism resulted from differential expressions of multiple fungi in the caterpillar body, stroma and ascocarp portion of Cordyceps sinensis (Cs).
Methods: Used the fuzzy, integral RAPD molecular marker polymorphism method with 20 random primers; used density-weighted cluster algorithms and ZUNIX similarity equations; compared RAPD polymorphisms of the caterpillar body, stroma and ascocarp of Cs during maturation; and compared RAPD polymorphisms of Cs and Hirsutella sinensis (Hs).
Results: Density-unweighted algorithms neglected the differences in density of the DNA amplicons.
[Maturation of Cordyceps sinensis associates with alterations of fungal expressions of multiple Ophiocordyceps sinensis mutants in stroma of Cordyceps sinensis].
Beijing Da Xue Xue Bao Yi Xue Ban
June 2012
Pharmanex Beijing Clinical Pharmacology Center, Beijing 100088, China.
Objective: To examine maturational changes in expressions of Ophiocordyceps sinensis (O.sinensis) transition and transversion mutation genotypes in Cordyceps sinensis (C.sinensis) stroma.
View Article and Find Full Text PDF[Detection of multiple Ophiocordyceps sinensis mutants in premature stroma of Cordyceps sinensis by MassARRAY SNP MALDI-TOF mass spectrum genotyping].
Beijing Da Xue Xue Bao Yi Xue Ban
April 2011
Pharmanex Beijing Clinical Pharmacology Center, Beijing 100088, China.
Objective: To examine the mutants of Ophiocordyceps sinensis (Os) in the stroma of premature Cordyceps sinensis (Cs).
Methods: Used MassARRAY single nucleotide polymorphism (SNP) MALDI-TOF mass spectrum genotyping, designed eight SNP extension primers on the basis of the scattered, multiple point mutations of known Os mutants within their internal transcribed spacer (ITS) segments, and examined the Os mutant genotypes relating to the GC-biased Os genotype (gb #AB067721) in premature Cs stroma.
Results: The two AT-biased genotypes and the GC-biased Os were simultaneously detected in premature Cs stroma.