Preparation, characterization and uptake of PEG-coated, muco-inert nanoparticles in HGC-27 cells, a mucin-producing, gastric-cancer cell line.

To prepare polyethylene glycol (PEG)-coated, muco-inert nanoparticles, we investigated the interaction between nanoparticles and mucin, and analyzed the cellular uptake of nanoparticles in HGC-27 cells, which are a mucin-producing, gastric cancer cell line. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were manufactured through a nanoprecipitation/emulsion evaporation technique, and coumarin-6 was encapsulated as a fluorescent marker. We characterized and evaluated the nanoparticles.