Ginsenoside Rg Reduces Cardiotoxicity While Increases Cardiotonic Effect of Aconitine in vitro.

Objective: To explore the synergic mechanism of ginsenoside Rg (Rg) and aconitine (AC) by acting on normal neonatal rat cardiomyocytes (NRCMs) and pentobarbital sodium (PS)-induced damaged NRCMs.

Methods: The toxic, non-toxic, and effective doses of AC and the most suitable compatibility concentration of Rg for both normal and damaged NRCMs exposed for 1 h were filtered out by 3- (4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide, respectively. Then, normal NRCMs or impaired NRCMs were treated with chosen concentrations of AC alone or in combination with Rg for 1 h, and the cellular activity, cellular ultrastructure, apoptosis, leakage of acid phosphatase (ACP) and lactate dehydrogenase (LDH), intracellular sodium ions [Na], potassium ions [K] and calcium ions [Ca] levels, and Nav1.

[Study on composition of essential oils in three different origins' Curcumae Rhizoma by AMDIS and retention index].

To compare the differences of essential oils extracted from Curcumae Rhizoma with different origins. The TIC of the essential oils of Curcumae Rhizoma from three different origins recorded by CP(2010) , were investigated by GC-MS combined with automated mass spectral deconvolution and identification system(AMDIS),steps as follow： firstly, overlapped peaks were resolved by AMDIS,secondly, NIST11.L standard MS spectral database combined with retention index were used to assist qualitative analysis, thirdly, the peak area of each split peak were determined by choosing the characteristic fragment ion peak, finally, the relative percentage contents of each compounds were determined through peak area normalization method.

[Comparative study of regulation of TLR/TRAF signaling pathway of classic prescriptions that deals with arthralgia syndrome based on relevent theory of prescription and syndrome].

Objective: Based on relevent theory of prescription and syndrome, to compare the gene expression differences of TLR2, TRAF6, and Faslg with adjuvant arthritis in rat spleen among Wutou decoction, Guizhi Shaoyao Zhimu decoction and Baihu Guizhi decoction.

Method: The experiment animal model of adjuvant arthritis in rats was established. Relative expression amount of TLR2, TRAF6, and Faslg in rats spleen was detected by SYBR Green I dye methods and implementation of fluorescence quantitative PCR technology with 18sRNA as an internal gene.

[HPLC fingerprint of Rhizoma Polygoni Cuspidati].

Objective: To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati (Polygonum cuspidatum).

Method: The HPLC separation was carried with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min.