Modeling Floral Induction in the Narrow-Leafed Lupin Under Different Environmental Conditions.

Flowering is initiated in response to environmental cues, with the photoperiod and ambient temperature being the main ones. The regulatory pathways underlying floral transition are well studied in but remain largely unknown in legumes. Here, we first applied an in silico approach to infer the regulatory inputs of four -like genes of the narrow-leafed lupin .

Transition from ballistic to diffusive heat transfer in a chain with breaks.

The transition from a ballistic to a diffusive regime of heat transfer is studied using two models. The first model is a one-dimensional chain with bonds, capable of dissociation. Interparticle forces in the chain are harmonic for bond deformations below a critical value, corresponding to the dissociation, and zero above this value.

3D-Printed Lithium-Ion Battery Electrodes: A Brief Review of Three Key Fabrication Techniques.

In recent years, 3D printing has emerged as a promising technology in energy storage, particularly for the fabrication of Li-ion battery electrodes. This innovative manufacturing method offers significant material composition and electrode structure flexibility, enabling more complex and efficient designs. While traditional Li-ion battery fabrication methods are well-established, 3D printing opens up new possibilities for enhancing battery performance by allowing for tailored geometries, efficient material usage, and integrating multifunctional components.

Characterization of Structural Properties and Antimicrobial Activity of the C3f Peptide of Complement System.

The C3f peptide is a by-product of regulation of the activated complement system with no firmly established function of its own. We have previously shown that C3f exhibits moderate antimicrobial activity against some Gram-positive bacteria . Presence of two histidine residues in the amino acid sequence of the peptide suggests enhancement of its antimicrobial activity at lower pH and in the presence of metal cations, particularly zinc cations.

mRNA encoding antibodies against hemagglutinin and nucleoprotein prevents influenza virus infection in vitro.

The emergence of new influenza virus strains presents a continuous challenge for global public health. mRNA technology offers a promising platform for rapidly developing therapeutics, particularly monoclonal antibodies, that can protect against viral infections. In this study, we engineered mRNA constructs encoding two types of antibodies: secreted antibodies specific to the hemagglutinin of the influenza A virus, based on previously characterized Fi6 antibodies, and intracellular Fab fragments targeting the nucleoprotein of the influenza B virus, derived from the 2/3 antibodies.

Stable Polymer-Lipid Hybrid Nanoparticles Based on -Polyhydroxyalkanoate and Cationic Liposomes for mRNA Delivery.

The development of polymer-lipid hybrid nanoparticles (PLNs) is a promising area of research, as it can help increase the stability of cationic lipid carriers. Hybrid PLNs are core-shell nanoparticle structures that combine the advantages of both polymer nanoparticles and liposomes, especially in terms of their physical stability and biocompatibility. Natural polymers such as polyhydroxyalkanoate (PHA) can be used as a matrix for the PLNs' preparation.

Cells have the ability to break and chemically modify GaP(As) nanowires.

Semiconductor nanowires are known for their unusual geometry, providing unique electronic and optical properties. Substrates with vertical nanowires have highly non-uniform surfaces, which are attractive in terms of the study of live cells that can interact and be labeled with the wires. Despite several previous works studying cells cultivated over nanowires, questions regarding cell rupture and interaction with the wires remain open.

Specific Features of Juvenile Idiopathic Arthritis Patients' Chemokine Profile: The Data of Case-Control Study Analysis.

Background: Juvenile idiopathic arthritis pathogenesis involves a large number of different immune system cells, which are both sources and targets of chemokines, that affect not only their migration but also survival, proliferation, differentiation, production of all cytokine types, degranulation, and also directly stimulating or suppressing angiogenesis. Studyingthe contribution of chemokines to this disease pathogenesis will make it possible to identify new sensitive and specific markers for its diagnosis and subsequent dynamic monitoring of treatment effectiveness. The study aimed to identify a list of the most informative diagnostic markers from a wide range of juvenile idiopathic arthritis patients' blood plasma chemokines.

Composite suture material based on polylactide accelerates the healing of surgical wounds in in vivo experiment.

In laboratory conditions, composite sutures based on polylactide (PLA) containing chitin nanofibrils modified with polyethylene glycol (CN-PEG) and poviargol (silver nanoparticles stabilized with poly(N-vinylpyrrolidone)) were obtained, studied, and used as a prototype. Surgical sutures threads with the addition of CN-PEG have stable mechanical properties both in air and in a buffer simulating the environment of a living organism. The yield strength of oriented threads decreased by an average of 15%, whereas for non-oriented threads the decrease was 3-4 times.

Advancements and Future Prospects in Molecular Targeted and siRNA Therapies for Chronic Myeloid Leukemia.

Chronic myeloid leukemia (CML) is an oncological myeloproliferative disorder that accounts for 15 to 20% of all adult leukemia cases. The molecular basis of this disease lies in the formation of a chimeric oncogene BCR-ABL1. The protein product of this gene, p210 BCR-ABL1, exhibits abnormally high constitutive tyrosine kinase activity.

Photonic bound states in the continuum governed by heating.

A photonic crystal microcavity with the liquid crystal resonant layer tunable by heating has been implemented. The multiple vanishing resonant lines corresponding to optical bound states in the continuum are observed. The abrupt change in the resonant linewidth near the vanishing point can be used for temperature sensing.

The archaeal highly thermostable GH35 family β-galactosidase DaβGal has a unique seven domain protein fold.

The most extensively studied β-d-galactosidases (EC3.2.1.

Reducing and tuning the work function of field emission nanocomposite CNT/NiO cathodes by modifying the chemical composition of the oxide.

This work presents for the first time the possibility of reducing and tuning the work function of field emission cathodes coated with metal oxides by changing the chemical composition of oxide coatings using an example of heat-treated CNT/NiO nanocomposite structures. These cathodes are formulated using carbon nanotube (CNT) arrays that are coated with ultrathin layers of nickel oxide (CNT/NiO) by atomic layer deposition (ALD). It was found that NiO at thicknesses of several nanometers grown on CNTs heat treated at a temperature of 350 °C can change its stoichiometric composition towards the formation of oxygen vacancies, since the Ni/Ni peak area ratio increases and the position of the Ni-O peak binding energies shifts as observed using X-ray photoelectron spectroscopy (XPS).

Antiplatelet Effects of Flavonoid Aglycones Are Mediated by Activation of Cyclic Nucleotide-Dependent Protein Kinases.

Flavonoid aglycones are secondary plant metabolites that exhibit a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, anticancer, and antiplatelet effects. However, the precise molecular mechanisms underlying their inhibitory effect on platelet activation remain poorly understood. In this study, we applied flow cytometry to analyze the effects of six flavonoid aglycones (luteolin, myricetin, quercetin, eriodictyol, kaempferol, and apigenin) on platelet activation, phosphatidylserine externalization, formation of reactive oxygen species, and intracellular esterase activity.

Chemical Constituents of Stinging Nettle ( L.): A Comprehensive Review on Phenolic and Polyphenolic Compounds and Their Bioactivity.

Polyphenolic compounds are of great interest in today's science. Naturally, they occur in plants and other sources in many different forms. Their wide range of biological activity has attracted the attention of the scientific community.

The investigation of the dynamics of changes in neutralizing antibody titers against type 5 adenovirus in the context of vaccination against a new coronavirus infection.

This research focuses on analyzing the dynamics of neutralizing antibody (nAbs) titers against type 5 adenovirus (Ad5) in the adult population of Russia following vaccination against the novel coronavirus infection with recombinant adenovirus type-5 COVID-19 vaccine (CanSino Biologics, China). The impact of the Ad5 vector on nAb titers was investigated using 302 blood serum samples from individuals who received a single dose of the Ad5-nCoV vector vaccine. The research revealed that 33.

Advanced technique of myocardial no-reflow quantification using indocyanine green.

The post-ischemic no-reflow phenomenon after primary percutaneous coronary intervention (PCI) is observed in more than half of subjects and is defined as the absence or marked slowing of distal coronary blood flow despite removal of the arterial occlusion. To visualize no-reflow in experimental studies, the fluorescent dye thioflavin S (ThS) is often used, which allows for the estimation of the size of microvascular obstruction by staining the endothelial lining of vessels. Based on the ability of indocyanine green (ICG) to be retained in tissues with increased vascular permeability, we proposed the possibility of using it to assess not only the severity of microvascular obstruction but also the degree of vascular permeability in the zone of myocardial infarction.

Modeling the Flowering Activation Motif during Vernalization in Legumes: A Case Study of .

In many plant species, flowering is promoted by the cold treatment or vernalization. The mechanism of vernalization-induced flowering has been extensively studied in but remains largely unknown in legumes. The orthologs of the gene, a major regulator of vernalization response in , are absent or non-functional in the vernalization-sensitive legume species.

Specific Features of Juvenile Idiopathic Arthritis Patients' Cytokine Profile.

Juvenile idiopathic arthritis (JIA) is a systemic autoimmune disease that affects the joints, leading to disability. Cytokines and signaling molecules expressed by the immune system cells play a key role in JIA pathogenesis. Understanding how their content changes during pathology development can open up new opportunities for its diagnosis and treatment.

Maternal Influenza and Offspring Neurodevelopment.

This review examines the complex interactions between maternal influenza infection, the immune system, and the neurodevelopment of the offspring. It highlights the importance of high-quality studies to clarify the association between maternal exposure to the virus and neuropsychiatric disorders in the offspring. Additionally, it emphasizes that the development of accurate animal models is vital for studying the impact of infectious diseases during pregnancy and identifying potential therapeutic targets.

How the immune mousetrap works: Structural evidence for the immunomodulatory action of a peptide from influenza NS1 protein.

One of the critical stages of the T-cell immune response is the dimerization of the intramembrane domains of T-cell receptors (TCR). Structural similarities between the immunosuppressive domains of viral proteins and the transmembrane domains of TCR have led several authors to hypothesize the mechanism of immune response suppression by highly pathogenic viruses: viral proteins embed themselves in the membrane and act on the intramembrane domain of the TCRalpha subunit, hindering its functional oligomerization. It has also been suggested that this mechanism is used by influenza A virus in NS1-mediated immunosuppression.

Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro.

Gene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional chronic myeloid leukemia (CML) tyrosine kinase inhibitor (TKIs) therapies, given the prevalence of clinical TKI resistance. The main challenge for such approaches remains the development of the effective delivery system for siRNA tailored to the specific disease model.

Atomic Layer Deposition of Antibacterial Nanocoatings: A Review.

In recent years, antibacterial coatings have become an important approach in the global fight against bacterial pathogens. Developments in materials science, chemistry, and biochemistry have led to a plethora of materials and chemical compounds that have the potential to create antibacterial coatings. However, insufficient attention has been paid to the analysis of the techniques and technologies used to apply these coatings.