7 results match your criteria: "Peter the Great Polytechnic University[Affiliation]"
Solid-State Nanopore-Based Nanosystem for Registration of Enzymatic Activity of a Single Molecule of Cytochrome P450 BM3.
Int J Mol Sci
October 2024
Institute of Biomedical Chemistry, 10, Pogodinskaya St., 119121 Moscow, Russia.
Article Synopsis
- Experimental methods in single-molecule enzymology enable scientists to analyze the unique properties and function of individual enzyme molecules during their catalytic processes.
- The study utilizes solid-state nanopores, specifically a 5 nm pore in a silicon nitride chip, to observe the performance of cytochrome P450 BM3, a model enzyme in monooxygenase systems.
- By measuring ion current changes while the enzyme catalyzes laurate hydroxylation, the research showed that the BM3 enzyme is active for up to 1500 seconds, with potential applications in developing sensitive detectors for enzyme studies.
Registration of Functioning of a Single Horseradish Peroxidase Macromolecule with a Solid-State Nanopore.
Int J Mol Sci
October 2023
Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia.
Currently, nanopore-based technology for the determination of the functional activity of single enzyme molecules continues its development. The use of natural nanopores for studying single enzyme molecules is known. At that, the approach utilizing artificial solid-state nanopores is also promising but still understudied.
View Article and Find Full Text PDFA new Caenorhabditis elegans model to study copper toxicity in Wilson disease.
Traffic
January 2024
Cell Biology and Disease Mechanisms Program, Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.
Article Synopsis
- Wilson disease (WD) is caused by mutations in the ATP7B gene, leading to copper overload in the liver and brain, which can result in severe health issues.
- A mutant strain of Caenorhabditis elegans was created to study this condition, showing significant Cu sensitivity, stunted development, and other health impairments due to a specific ATP7B variant.
- The cua-1 mutant strain serves as a valuable experimental model for understanding copper toxicity in WD and testing potential therapeutic approaches.
Predicting novel disease mutations in the cardiac sodium channel.
Biochem Biophys Res Commun
January 2020
Almazov National Medical Research Centre, St. Petersburg, 197341, Russia; Department of Bioinformatics, Peter the Great Polytechnic University, St. Petersburg, 195251, Russia; Department of Bioinformatics, Technical University of Munich, Wissenschaftszentrum Weihenstephan, Maximus-von-Imhof Forum 3, 85354, Freising, Germany. Electronic address:
Background: Voltage-gated sodium channels Nav1.x mediate the rising phase of action potential in excitable cells. Variations in gene SCN5A, which encodes the hNav1.
View Article and Find Full Text PDFSyntax meets semantics during brain logical computations.
Prog Biophys Mol Biol
December 2018
Dept. Psychology and Dept. Engineering, Northeastern University, Boston, MA, USA; Peter the Great Polytechnic University, St. Petersburg, Russia. Electronic address:
The discrepancy between syntax and semantics is a painstaking issue that hinders a better comprehension of the underlying neuronal processes in the human brain. In order to tackle the issue, we at first describe a striking correlation between Wittgenstein's Tractatus, that assesses the syntactic relationships between language and world, and Perlovsky's joint language-cognitive computational model, that assesses the semantic relationships between emotions and "knowledge instinct". Once established a correlation between a purely logical approach to the language and computable psychological activities, we aim to find the neural correlates of syntax and semantics in the human brain.
View Article and Find Full Text PDFPhysics of mind: Experimental confirmations of theoretical predictions.
Phys Life Rev
August 2018
Peter the Great Polytechnic University, St. Petersburg, Russia. Electronic address:
What is common among Newtonian mechanics, statistical physics, thermodynamics, quantum physics, the theory of relativity, astrophysics and the theory of superstrings? All these areas of physics have in common a methodology, which is discussed in the first few lines of the review. Is a physics of the mind possible? Is it possible to describe how a mind adapts in real time to changes in the physical world through a theory based on a few basic laws? From perception and elementary cognition to emotions and abstract ideas allowing high-level cognition and executive functioning, at nearly all levels of study, the mind shows variability and uncertainties. Is it possible to turn psychology and neuroscience into so-called "hard" sciences? This review discusses several established first principles for the description of mind and their mathematical formulations.
View Article and Find Full Text PDFA rapid two-dimensional data collection system for the study of ferroelectric materials under external applied electric fields.
J Appl Crystallogr
October 2016
School of Materials, The University of Manchester, Oxford Road, Manchester M13 9PL, UK; ESRF, 71 Rue des Martyrs, Grenoble, France.
Synchrotron X-rays on the Swiss Norwegian Beamline and BM28 (XMaS) at the ESRF have been used to record the diffraction response of the PMN-PT relaxor piezoelectric 67% Pb(MgNb)O-33% PbTiO as a function of externally applied electric field. A DC field in the range 0-18 kV cm was applied along the [001] pseudo-cubic direction using a specially designed sample cell for single-crystal diffraction experiments. The cell allowed data to be collected on a Pilatus 2M area detector in a large volume of reciprocal space using transmission geometry.
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