[Isolation, culture and biological characterization of Beagle stem cells from apical papilla].

Objective: To isolate and characterize the Beagle stem cells from apical papilla.

Methods: Apical papilla was severed from the end of freshly extracted Beagle's young permanent upper anterior teeth, and digested by collagenase type I and dispase for cell culture. The isolated cells were investigated for stem cell properties by analyzing their colony-forming efficiency, growth characteristics and the expression of mesenchymal stem cell markers; and evaluating their multidifferentiation potentials including osteogenic, adipogenic, and chondrogenic potentials.