Cannabichromene from full-spectrum hemp extract exerts acute anti-seizure effects through allosteric activation of GABA receptors.

The approval of Epidiolex, an anti-epileptic drug containing cannabidiol (CBD) as its active component, has brought hope to patients with refractory epilepsy. However, the anti-seizure effect of full-spectrum hemp extract (HE), a CBD-enriched hemp oil, remains unclear. In this study, we investigated the anti-seizure effect of HE using drug-induced seizure models.

Geographic and socioeconomic disparities in mortality burden attributable to long-term exposure to NO across 231 cities in China from 2015 to 2019.

Research on geographic and socioeconomic disparities of NO attributed mortality burden is limited. This study aims to quantify the geographic and socioeconomic differences in the association between long-term exposure to NO and mortality burden in China. We estimated the all-cause mortality burden of adults over 16 years old attributable to NO exposure above 10 µg/m for 231 Chinese cities from 2015 to 2019, and geographic and socioeconomic differences .

Effects of smoking cessation on individuals with COPD: a systematic review and meta-analysis.

Objective: Despite smoking being a significant risk factor in the occurrence and progression of chronic obstructive pulmonary disease (COPD), no comprehensive analysis has been conducted to determine the potential benefits of smoking cessation for patients with established COPD or identify specific indicators that may be improved. The aim of our meta-analysis was to elucidate the positive impact of smoking cessation on COPD.

Methods: We conducted a comprehensive search of PubMed, EMBASE, Web of Science, Cochrane Library, CNKI, Wan Fang and VIP databases to identify studies that met our eligibility criteria from inception up to 1, May 2024.

Natural antibodies to polysaccharide capsules enable Kupffer cells to capture invading bacteria in the liver sinusoids.

The interception of blood-borne bacteria in the liver defines the outcomes of invasive bacterial infections, but the mechanisms of this antibacterial immunity are not fully understood. This study shows that natural antibodies (nAbs) to capsules enable liver macrophage Kupffer cells (KCs) to rapidly capture and kill blood-borne encapsulated bacteria in mice. Affinity pulldown with serotype-10A capsular polysaccharides (CPS10A) of Streptococcus pneumoniae (Spn10A) led to the identification of CPS10A-binding nAbs in serum.

Interventions to support the psychological empowerment of nurses: a scoping review.

Background: Establishing an empowering work environment is significantly contributing to nurse's job satisfaction, performance, retention, and organizational success. This study aimed to conduct a scoping review to chart and synthesize current research on interventions to support nurses' psychological empowerment.

Methods: Ten databases were searched, including PubMed/Medline, Web of Science, Scopus, Embase, EBSCOhost, Cochrane Library, CNKI, Wanfang, VIP, and OpenGrey, following the Joanna Briggs Institute's methodology for scoping reviews.

N-glycosylation Modification of CTSD Affects Liver Metastases in Colorectal Cancer.

Liver metastasis is the primary factor contributing to unfavorable prognosis in colorectal cancer (CRC). Although N-glycosylation is implicated in metastasis, there is a notable paucity of comprehensive studies addressing the N-glycosylation proteomics associated with liver metastasis in CRC. In this study, N-glycosylated proteins and N-glycosylation sites of differential expression between primary lesions and paired liver metastatic lesions are identified.

Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.

Three-dimensional structured illumination microscopy (3D-SIM) provides excellent optical sectioning and doubles the resolution in all dimensions compared with wide-field microscopy. However, its much lower axial resolution results in blurred fine details in that direction and overall image distortion. Here we present 4Pi-SIM, a substantial revamp of IS that synergizes 3D-SIM with interferometric microscopy to achieve isotropic optical resolution through interference in both the illumination and detection wavefronts.

Behavior-change lifestyle interventions for the treatment of obesity in children and adolescents: A scoping review.

Behavior-change lifestyle interventions are fundamental in children and adolescent obesity management. This scoping review discusses optimal behavior-change lifestyle interventions in the treatment of overweight and obesity in children and adolescents. A literature search on diet, physical activity, and behavioral intervention for obesity treatment in children and adolescents aged 0-19 years was conducted in the Cochrane Library, MEDLINE (OVID), EMBASE, and ClinicalTrials.

DDX39A resolves replication fork-associated RNA-DNA hybrids to balance fork protection and cleavage for genomic stability maintenance.

Safeguarding replication fork stability in transcriptionally active regions is crucial for precise DNA replication and mutation prevention. Here, we discover the pervasive existence of replication fork-associated RNA-DNA hybrids (RF-RDs) in transcriptionally active regions of human cells. These hybrids function as protective barriers, preventing DNA2-mediated nascent DNA degradation and replication fork collapse under replication stress.

HCC Model Induced by P53 and Pten Knockout in HBV-Transgenic Mice Mirrors Human HCC at the Transcriptome Level.

With a multitude of HCC mouse models available, choosing the one that most closely resembles human HCC can be challenging. This study addresses this gap by conducting a comprehensive transcriptomic similarity analysis of widely used HCC mouse models. In this study, RNA-seq was performed on a model induced by double knockout of P53 and Pten via CRISPR/Cas9 in HBV-transgenic mice.

One-Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point-of-Care Testing.

Hepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, and easy-to-use detection. This study aimed to develop a One-Pot assay using CRISPR/Cas13a for detecting HEV RNA, suitable for point-of-care testing (POCT) in resource-limited settings. CRISPR/Cas13a combined with reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription recombinase-aided amplification (RT-RAA) was applied to a One-Pot assay device.

Association of dietary inflammatory index and refractive status in American adolescents: NHANES 1999-2008.

Background: Several nutrients have been found to be associated with the prevalence of myopia, and the role of dietary patterns in influencing myopia risk has recently garnered significant attention. We aim to explore the relationship between the Dietary Inflammatory Index (DII) and refractive status in adolescents.

Methods: Data from 7,331 participants were analyzed from the 2005-2008 US National Health and Nutrition Examination Survey (NHANES).

retinal melanin detection with the calibrated depolarization index in polarization-sensitive optical coherence tomography.

Significance: A data-based calibration method with enhanced depolarization contrast in polarization-sensitive optical coherence tomography (PS-OCT) was developed and demonstrated effective for detecting melanin content in the eye.

Aim: We aim to mitigate the dependence between the measured depolarization metric and the intensity signal-to-noise ratio (SNR) for improved visualization of depolarizing tissues, especially in low SNR regions, and to demonstrate the enhanced depolarization contrast to evaluate melanin presence.

Approach: A function for calibrating the depolarization metric was experimentally derived from the young albino guinea pig, assuming depolarization free in the retina.

Effective Hydrogel Vascular Patch Dual-Loaded with Cycloastragenol Nanostructured Lipid Carriers and Doxycycline for Repairing Extravascular Injury in Abdominal Aortic Aneurysm.

Endovascular aneurysm repair (EVAR) plays a crucial role in the treatment of abdominal aortic aneurysm (AAA) in the clinic, but the aneurysm remains in the patient's body after surgery, continuing to pose a risk of progression. Cycloastragenol (CAG) is proven to be an effective anti-AAA drug, and its vascular protective effects can be further improved when the hydrophobic CAG is encapsulated into nano-sized formulations to enhance its bioavailability. In this context, this study developed an extravascular patch hydrogel loaded with CAG nanostructured lipid carriers and a hydrophilic drug of doxycycline hydrochloride (DOX).

Single-cell atlas comparison across vertebrates reveals auditory cell evolution and mechanisms for hair cell regeneration.

Mammals suffer permanent hearing impairment from the loss of auditory hair cells due to their inability to regenerate. In contrast, lower vertebrates exhibit extraordinary capacity for hair cell regeneration and hearing restoration, but the mechanisms remain unclear. Here we characterize the single-cell atlas of Xenopus laevis inner ear and perform a comprehensive comparison with mouse model.

Situational leadership theory in nursing management: a scoping review.

Aim: To provide a comprehensive overview of situational leadership theory as applied to nursing management, outlining its relevant measurement tools, intervention modalities, and reporting gaps.

Design: A scoping review of the literature was conducted according to the JBI guidelines.

Data Sources: Eight databases were used including PubMed, Embase, CINHAL, Web of Science, CNKI, WanFang, Cqvip, SinoMed.

Irisin reprograms microglia through activation of STAT6 and prevents cognitive dysfunction after surgery in mice.

Postoperative cognitive dysfunction (POCD) is common in the aged population and associated with poor clinical outcomes. Irisin, an endogenous molecule that mediates the beneficial effects of exercise, has shown neuroprotective potential in several models of neurological diseases. Here we show that preoperative serum level of irisin is reduced in dementia patients over the age of 70.

Nurses' job embeddedness and turnover intention: A systematic review and meta-analysis.

Objective: This study aimed to review the relationship between job embeddedness and turnover intentions among nurses and explore the effects of the Job Embeddedness Scale, number of years in the career, education, and marital status on this relationship.

Methods: The review was conducted by searching the China Knowledge Resource Integrated Database (CNKI), Weipu Database (CQVIP), China Biology Medicine (CBM), Wanfang Database, PubMed, Web of Science, Embase, CINAHL, and APA-PsycNet for articles on nurses' job embeddedness and turnover from intention up to March 2024. The research quality was evaluated using the Agency for Healthcare Research and Quality (AHRQ) assessment criteria.

Engineering Mild-Photothermal Responsive and NO Donor Prussian Blue Nanozymes Using Mild Synthesis for Inflammation Regulation and Bacterial Eradication in Periodontal Disease.

Periodontitis, an infectious disease of periodontal tissues caused by oral bacterial biofilms, is characterized by reactive oxygen species (ROS) accumulation and immune microenvironment imbalance. Multifunctional nanozymes, leveraging their physiochemical properties and enzymatic activities, offer promising antibacterial and anti-inflammatory strategies for managing periodontitis. In particular, Prussian blue nanozymes (PBzymes) exhibit exceptional ROS control due to their robust catalytic activity, diverse antioxidant functions, and high biocompatibility.

Uncovering the Differed Susceptibility of ( and ) to Fungicide Phenamacril: From Computational and Experimental Perspectives.

and are two subtypes of (Fo), a pathogenic filamentous fungus. Phenamacril (PHA), a -specific fungicide that targets myosin I, exhibits significant hyphal growth inhibition in but shows weak resistance in , despite only two amino acid differences in the PHA-binding pocket of myosin I. In this study, we aim to elucidate the molecular basis for the differential sensitivity of myosin I variants (FoMyoI and FoMyoI) to phenamacril through computational methods and biochemical validation.

Economic evaluation of childhood pneumococcal conjugate vaccination programs in Qinghai and Hainan provinces, China.

Background: The burden of pneumococcal disease varies regionally in China, disproportionately affecting children in many provinces such as Qinghai and Hainan. Nevertheless, the absence of a pneumococcal conjugate vaccine (PCV) in the National Immunization Program (NIP) or local programs presents limited coverage. This study evaluated the health and economic impact of including PCV in immunization programs in Qinghai and Hainan.

Promoting Influenza Vaccination Uptake Among Chinese Older Adults Based on Information-Motivation-Behavioral Skills Model and Conditional Economic Incentive: Protocol for Randomized Controlled Trial.

: Influenza poses a substantial health burden, especially among older adults in China. While vaccination is one of the most effective preventions, influenza vaccine uptake rates among Chinese older adults remain low. This study examines the individual and combined effects of behavioral interventions based on the Information-Motivation-Behavioral Skills (IMB) model and economic incentives in promoting influenza vaccine uptake among older adults living in China.

Chemogenetic Modulation of Preoptic Neurons Decreases Body Temperature and Heart Rate.

The preoptic area of the hypothalamus is critical for regulation of brain-body interaction, including circuits that control vital signs such as body temperature and heart rate. The preoptic area contains approximately 70 molecularly distinct cell types. The gene is expressed in a subset of preoptic area cell types.

Deep Saliva Proteomics Elucidating the Pathogenesis of Early Childhood Caries and Identifying Biomarkers for Early Prediction.

Early childhood caries (ECC) significantly impacts the physical and mental health of children. Saliva stands as a critical model for investigating the pathogenesis of caries disease since it reflects both host and microbial dynamics. However, the specific contributions of salivary host factors to ECC have not been fully understood.