1 results match your criteria: "Peking University Center for Organ Transplantation[Affiliation]"
[Effect of rapamycin in inducing naïve murine effector T cell convert to regulatory T cell].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
August 2008
Department of Hepatobiliary Surgery, Peking University People's Hospital, Peking University Center for Organ Transplantation, Beijing 100044, China.
Objective: To study the effect of rapamycin in inducing naïve murine effector T cell (Teff) convert to regulatory T cell (Treg) in vitro.
Methods: The forkhead box protein 3 (FoxP3) negative Teff were isolated and purified from the spleen and lymph node of C57 BL/6 murines aged 6-8 weeks, then Teff were cultured in three groups with mature dendritic cells (mDC), B cells, and plate coated Anti-CD3. In addition, the control wells and the test wells were prepared in each group, rapamycin were not added in the control wells but added in the test wells with concentrations of 1, 10, 50, and 100 nmol/L.