Zanubrutinib plus Ixazomib and Dexamethasone in newly diagnosed symptomatic Waldenström macroglobulinemia:a phase II study.

Purpose: Waldenström macroglobulinemia (WM) is a rare type of lymphoma, with no optimal treatment. BTK inhibitor have shown promising outcomes, yet achieving deep remission (VGPR or CR) remains challenging and time-limited therapy with proteasome-inhibition has not been reported. We conducted a phase 2 clinical trial (NCT04463953) to evaluate the efficacy and safety of combining zanubrutinib, ixazomib and dexamethasone (ZID) in newly diagnosed WM patients.

Efficacy of Doxycycline-Sitafloxacin Sequential Therapy for Urogenital Mycoplasma genitalium Infection in Nanjing, China.

Background: The aim of this study was to evaluate the efficacy of doxycycline-sitafloxacin sequential therapy in the treatment of Mycoplasma genitalium (Mg) urogenital infections in Nanjing, China.

Methods: Potential subjects were tested initially for Mg infection by nucleic acid amplification testing and again at least 21 days after completion of doxycycline (100 mg twice daily for 7 days)-sitafloxacin (100 mg twice daily for 7 days) sequential therapy. The presence of macrolide and quinolone resistance-associated mutations in 23S rRNA, parC, gyrA, and gyrB genes in Mg was examined at baseline and upon retesting of specimens from subjects that did not clear Mg.

Amyloid pathology related to aberrant structure-function coupling of brain networks in Alzheimer's disease: insights from [F]-florbetapir PET imaging.

Purpose: Brain structure-function coupling (SFC), which reflects the degree to which anatomical structure supports neural function, is an emerging imaging marker in neurodegenerative diseases. However, its pathological underpinnings in Alzheimer's disease (AD) remain poorly understood. This study aimed to examine the association among amyloid pathology, SFC disruption and cognitive decline.

Flow-cytometric lymphocyte subsets enumeration: comparison of single/dual-platform method in clinical laboratory with dual-platform extended PanLeucogating method in reference laboratory.

Objectives: To improve the accuracy of lymphocyte subsets counts, a traceable dual-platform extended PanLeucogating method (DPP) of absolute cell counts of lymphocyte subsets was introduced, and consistency was evaluated by comparing conventional single/dual-platform method with DPP.

Methods: The DPP for absolute lymphocyte subsets counts was established by multiplying the percentage of lymphocyte subsets in total white blood cells (WBC) measured by flow cytometer with the total WBC counts. DPP-R was defined as the use of the total WBC counts measured in reference laboratory that was traceable to reference method recommended by International Council for Standardization in Hematology (ICSH).

Clofazimine enhances anti-PD-1 immunotherapy in glioblastoma by inhibiting Wnt6 signaling and modulating the tumor immune microenvironment.

Glioblastoma multiforme (GBM) is an aggressive and lethal primary brain tumor with limitedtreatment options due to its resistance to conventional therapies and an immunosuppressive tumor microenvironment. In this study, we investigated whether clofazimine, an inhibitor of the Wnt/β-catenin signaling pathway, could enhance the efficacy of anti-PD-1 immunotherapy in GBM. Our in vitro and in vivo experiments demonstrated that clofazimine suppressed GBM cell proliferation, induced apoptosis, and inhibited invasion by downregulating Wnt6-mediated activation of the Wnt/β-catenin pathway and the downstream MEK/ERK signaling cascade, leading to decreased PD-L1 expression.

Coptisine ameliorates colitis in mice by modulating cPLA2/TRPM8/CGRP-1 signaling pathways and strengthening intestinal barrier function.

Coptisine (COP), a naturally occurring alkaloid, is recognized for its varied pharmacological impacts and its supportive function in intestinal well-being. However, the role of COP to protect the colonic epithelium in colitis has not been extensively investigated. The objective of this study was to assess the efficacy of COP in ameliorating colitis by investigating intestinal histopathology, mucosal barrier function, and transient receptor potential (TRP) signaling pathways in mice with colon disease compared to a control group, thereby elucidating the underlying mechanisms of its action.

An Intervertebral Disc (IVD) Regeneration Model Using Human Nucleus Pulposus Cells (iHNPCs) and Annulus Fibrosus Cells (iHAFCs).

Intervertebral disc (IVD) degeneration (IVDD), primarily caused by nucleus pulposus (NP) dehydration, leads to low back pain. While current treatments focus on symptom management or surgical intervention, tissue engineering using IVD-derived cells, biofactors, and scaffolds offers a promising regenerative approach. Here, human NP cells (NPCs) and annulus fibrosus cells (AFCs) are immortalized with human telomerase reverse transcriptase (hTERT), generating immortalized NPCs (iHNPCs) and AFCs (iHAFCs).

Choroidal changes and association with Gaussian curvature in high myopic eyes with posterior staphyloma in ultra-wide-field optical coherence tomography angiography.

Purpose: To evaluate choroidal changes in high myopic (HM) patients with posterior staphyloma (PS) and explore their association with Gaussian curvature (K) using ultra-wide-field optical coherence tomography angiography (UWF-OCTA).

Methods: HM patients with wide macular PS (Type I PS) and without any PS (control) were recruited. Highest K values (K) and choroidal parameters-vessel flow density (VFD), choroidal vascularity volume (CVV) and index (CVI), thickness, and volume-were compared in central and peripheral regions, and the fovea-centered 12-mm ETDRS quadrants of 24 × 20 mm UWF-OCTA images.

Polygenic Risk Score for the Efficacy of Clopidogrel in Patients With Minor Stroke or Transient Ischemic Attack: A Post Hoc Analysis of the CHANCE Trial.

Background: Dual antiplatelet therapy (DAPT) with clopidogrel and aspirin is recommended for secondary prevention in patients with a minor stroke or transient ischemic attack. However, the effectiveness of DAPT can be significantly influenced by genetic variations. This study aimed to estimate the impact of multiple single-nucleotide polymorphisms across various genes on DAPT efficacy using polygenic risk score (PRS).

The efficacy comparison on femoral vein hemodynamics and morphology between two types of graduated elastic compression stockings among patients undergoing knee replacement: a single center, double-blind, randomized trial.

Background: Venous thromboembolism is a significant complication after knee replacement. The short-term efficacy disparities between different types of graduated elastic compression stockings (GCS) among patients undergoing total/unicompartmental knee replacement remain unclear.

Objective: The aim of the trial was to compare the efficacies on hemodynamics and morphology of femoral vein between two types of GCS, providing more evidence on GCS prophylaxis among patients undergoing total/unicompartmental knee replacement.

Construction of multiple and mixed valvular heart disease-related age-adjusted comorbidity index and its predictive value for patient prognosis.

Objectives: To create a multiple and mixed valvular heart disease (MVHD)-related age-adjusted comorbidity index (MVACI) for predicting mortality risk of patients with MVHD.

Methods: A total of 4080 patients with moderate or severe MVHD in the China-VHD study were included. The primary endpoint was 2-year all-cause mortality.

Retroperitoneal Capillary Hemangioma Mimicking Paraganglioma on 18F-AlF-NOTA-Octreotide PET/CT.

Capillary hemangiomas are commonly encountered superficially in the cutaneous, subcutaneous, or mucosal tissues during childhood and early adulthood, but retroperitoneal capillary hemangioma is a rare occurrence. We present a case of a 61-year-old woman with incidentally detected capillary hemangioma in the retroperitoneum initially presumed to be paraganglioma based on 18F-AlF-NOTA-Octreotide PET/CT findings. Contrast-enhanced MRI revealed a hypervascular mass in the retroperitoneum.

Association of the atherogenic index of plasma with frailty in U.S. adults: a cross-sectional study based on NHANES.

Background: Frailty is a multifactorial syndrome associated with adverse health outcomes. The metabolic underpinnings of frailty, particularly lipid metabolism, are not fully understood. Unlike isolated lipid fractions or inflammatory markers, atherogenic index of plasma (AIP) integrates atherogenic lipid profiles and systemic inflammation.

Application of family-centered care in cardiac surgery: a scoping review.

Background: Family-centered care (FCC) has been widely implemented in various medical settings, its application in cardiac surgery remains underexplored. Given the complexity of cardiac surgery and the significant impact on both patients and families, understanding FCC's role in this context is essential to improving patient outcomes and family well-being.

Objectives: To identify the current state of the application of FCC in the families of cardiac surgery.

Association between waist-to-hip ratio and testosterone in Chinese men with young-onset type 2 diabetes: a cross-sectional study.

Background: Low testosterone levels is associated with higher cardiovascular risk in men with diabetes. However, there are few studies on testosterone levels and the factors affecting them in patients with young-onset diabetes (YOD). The objective of this study was to investigate the correlation between waist-to-hip ratio (WHR) and testosterone levels in men diagnosed with YOD.

Association between grip strength level and fall experience among older Chinese adults: a cross-sectional study from the CHARLS.

Objectives: To explore the dose-response relationship between levels of grip strength and the fall experience among older adult Chinese.

Methods: This study used data from the 2015 China Health and Retirement Longitudinal Study (CHARLS), including 5,486 older Chinese adults aged 60 and above. Grip strength was measured with a dynamometer, and falls were recorded via questionnaire.

Poor reporting quality and high proportion of missing data in economic evaluations alongside pragmatic trials: a cross-sectional survey.

Background: Lack of data integrity is a common problem in randomized clinical trials and is more serious in economic evaluations conducted alongside explanatory clinical trials. Despite pragmatic randomized controlled trials (pRCTs) becoming recognized as the best design for economic evaluations, information on the proportion, handling approaches, and reporting quality of missing data in pRCTs-based economic evaluations remains limited. This study aimed to investigate the quantity and reporting quality of missing data in economic evaluations conducted alongside pragmatic clinical trials.

Automatic detecting multiple bone metastases in breast cancer using deep learning based on low-resolution bone scan images.

Whole-body bone scan (WBS) is usually used as the effective diagnostic method for early-stage and comprehensive bone metastases of breast cancer. WBS images with breast cancer bone metastasis have the characteristics of low resolution, small foreground, and multiple lesions, hindering the widespread application of deep learning-based models. Automatically detecting a large number of densely small lesions on low-resolution WBS images remains a challenge.

Leveraging Deep Learning in Real-Time Intelligent Bladder Tumor Detection During Cystoscopy: A Diagnostic Study.

Background: Accurate detection of bladder lesions during cystoscopy is crucial for early tumor diagnosis and recurrence monitoring. However, conventional visual inspection methods have low and inconsistent detection rates. This study aimed to evaluate the effectiveness of the HRNetV2 deep learning model for intelligent bladder lesion detection, focusing on its performance at different image resolutions.

Mitochondria-enriched hematopoietic stem cells exhibit elevated self-renewal capabilities, thriving within the context of aged bone marrow.

The aging of hematopoietic stem cells (HSCs) substantially alters their characteristics. Mitochondria, essential for cellular metabolism, play a crucial role, and their dysfunction is a hallmark of aging-induced changes. The impact of mitochondrial mass on aged HSCs remains incompletely understood.

A New Medium That Promotes Mass Sporulation of Trichophyton rubrum In Vitro.

Background: Trichophyton rubrum is one of the most common human pathogenic fungi, with the infections often managed through empirical drug use. However, treatments frequently lead to relapse upon withdrawal. The rise in drug-resistant T.

[Progress in the study of biological factors of aqueous humor in patients with diabetic retinopathy].

Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus and a leading cause of vision loss in diabetic patients. Pathological processes such as inflammatory response, oxidative stress, and vascular dysfunction play important roles in the pathogenesis of DR. Changes in the levels of cytokines associated with these pathologic processes correlate with the onset, progression, and extent of DR.

[Fatal opportunistic infection occurred during the treatment of immune checkpoint inhibitor-associated colitis:case report].

Immune checkpoint inhibitor (ICI) has been widely used in lung cancer patients. While ICI treatment improves the overall survival of patients, it can also lead to a series of immune-related adverse events (irAE). Severe irAE should be treated with glucocorticoids and biological agents.