Genetic Variants and Haplotype Structures in the Gene Family to Predict Cancer Risk: A Bioinformatics Study.

Background And Aims: The cancer susceptibility () gene family of long noncoding RNAs (lncRNAs) plays an important role in cancer. The aim of this study was to identify genetic variants and haplotype structures of genes associated with cancer risk.

Methods: Genome-wide association studies (GWAS) significant variants ( ≤ 5 × 10) on family genes were identified from the GWAS Catalog-EMBL-EBI, and then cancer-associated variants on genes were extracted.

Embryonic stem cell differentiation to primordial germ cell like cells by Nigella sativa, Brassica Oleracea and Oenothera biennis extracts.

Objectives: This study aimed to investigate the induction effects of methanolic extracts of Nigella sativa (NiS), Brassica Oleracea (BrO), and Oenothera biennia (Obi) on transgenic embryonic stem cells (ESCs) and to evaluate the ability of germ cells (GCs) production using these pluripotent cells.

Methods: ESCs were amplified using a feeder layer. Embryoid bodies enzymatically dissociated to single cells and induced the extracts in gelatinized plates.