Mycofabrication of AgONPs derived from Aspergillus terreus FC36AY1 and its potent antimicrobial, antioxidant, and anti-angiogenesis activities.

Background: There is an emergency need for the natural therapeutic agents to treat arious life threatening diseases such as cardio- vascular disease, Rheumatoid arthritis and cancer. Among these diseases, cancer is found to be the second life threatening disease; in this view the present study focused to synthesize the silver oxide nanoparticles (AgONPs) from endophytic fungus.

Methods: The endophytic fungus was isolated from a medicinal tree Aegle marmelos (Vilva tree) and the potential strain was screened through antagonistic activity.

Nasal PCR assay for the detection of Mycobacterium leprae pra gene to study subclinical infection in a community.

Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Identification of Mycobacterium leprae is difficult in part due to the inability of the leprosy bacillus to grow in vitro. A number of diagnostic methods for leprosy diagnosis have been proposed.

Treatment and control of infestation in a conventional guinea pig () breeding colony.

A case of sarcoptic mange caused by in a conventional guinea pig breeding colony is reported. The infestation was reported in a large colony of guinea pigs during the month of July, 2013 affecting 30 breeder guinea pigs. Severely infested animals were treated individually with subcutaneous injection of ivermectin 1 % w/v (Neomec) at the rate of 400 µg/kg body weight 10 days apart.

Protection of mice against gastric colonization of Helicobacter pylori by therapeutic immunization with systemic whole cell inactivated vaccines.

The protective effect of therapeutic immunization with heat inactivated Helicobacter pylori cells administered with aluminum phosphate as an adjuvant was evaluated with "Swiss albino mice" infected with H. pylori Sydney strain 1 (SS1). The presence of bacteria in histological sections of the stomach was evaluated to confirm the colonization of H.

Isolation of fourth-instars larva of Aedes (Finlaya) harveyi (Diptera: Culicidae) from the Nilgiri hills, Southern India.

During the post monsoon season of 2012, the ovitraps were employed for dengue vector surveillance nearer to human habitations in the Nilgiri hills of Southern India. All the eggs obtained were brought to laboratory, and reared individually to adult stage for identification. A total of 30 exuviae of fourth-instars larva specimen were identified as Aedes (Finlaya) harveyi which were compared to other closely related species.

Use of scanning electron microscopy to confirm the identity of tropical rat mite (Ornithonyssus bacoti): the cause of rat mite dermatitis.

Cutaneous lesions in human patient due to the bite of rat mite Ornithonyssus bacoti are frequently misdiagnosed as allergies, fungal infection, or bacterial infection. Bite lesions in the personnel working in a Laboratory Animal facility which was infested with O. bacoti is reported here along with its therapeutic management.

Record of Tropical Rat Mite, Ornithonyssus bacoti (Acari: Mesostigmata: Macronyssidae) from Domestic and Peridomestic Rodents (Rattus rattus) in Nilgiris, Tamil Nadu, India.

Background: Tropical rat mite (Ornithonyssus bacoti) is reported from many parts of the world and is considered important in transmitting rickettsial pathogens. There have been scanty reports on prevalence of this parasite from India. Following a recent report of O.

Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine.

Aim: The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine.

Materials And Methods: Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated for pulmonary immune response in BAL fluid.

Comparative efficacy of fluorescent antibody test, immunoperoxidase test and enzyme linked immuno sorbent assay in detection of rotavirus in cell culture.

Rotavirus is prevalent worldwide and has been established as a leading cause of mortality due to severe diarrhoea in neonates. Isolation of the virus is a gold standard method for confirmation of rotavirus infection in the host. Propagation of rotavirus in cell culture is a challenge as in many instances the virus does not produce detectable cytopathic effect.

Standardization and assessment of cell culture media quantities in roller poly ethylene terephthalate bottles employed in the industrial rabies viral vaccine production.

Vero cells are utilized for production of rabies vaccine. This study deals with the optimize quantity media require for the rabies vaccine production in the smooth roller surface. The rabies virus (Pasteur vaccine strain) is infected to monolayer of the various experimented bottles.

Process standardization for optimal virus recovery and removal of substrate DNA and bovine serum proteins in Vero cell-derived rabies vaccine.

Purification of a rabies vaccine by a single zonal centrifugation run was replaced by two runs with optimal standardization of the sucrose density gradient. As a result, significant reductions in the levels of substrate DNA and bovine serum protein in the Vero cell-derived human rabies vaccine were achieved. Following many trials, for the first run, loading of the 3.

Tangential flow filtration technology applicable to large scale recovery of diphtheria toxin.

The commercial preparation of purified diphtheria toxoid primarily depends on the effective separation of the toxin from a large culture volume of Corynebacterium diphtheriae vaccine strain. Tangential flow or cross-flow filtration is gaining momentum in the processing of biologicals. This study deals with the separation of toxins using a microporous membrane (0.

Process optimization for enhanced production of diphtheria toxin by submerged cultivation.

When stationary culture was replaced by submerged cultivation in a fermentor, a significant increase in the yield of diphtheria toxin in a short cultivation time (less than 48 h as against 7-8 d) was noted. It was found that under optimal conditions of temperature, vortex mixing and surface aeration, an alkaline pH favoured toxin release. Furthermore, to enhance the production volume, two-and three-step semicontinuous batch cultivations were carried out.

Purification, potency and immunogenicity analysis of Vero cell culture-derived rabies vaccine: a comparative study of single-step column chromatography and zonal centrifuge purification.

Continuous Vero cell lines are more suitable for large-scale production of rabies vaccine. The purification of Vero cell-derived rabies vaccine is critical because of the residual cellular DNA and serum proteins. The perfection of techniques using column chromatography with different matrix material, gel filtration and zonal centrifugation is of paramount importance for the optimal purification of rabies vaccine, leaving minimal residual cellular DNA, below the permissible level of 100 pg per dose and serum protein content of 1 ppm.

Rapid fluorescent focus inhibition test for rabies antibody estimation using murine neuroblastoma cell line.

A rapid fluorescent focus inhibition test (RFFIT) for rabies antibody estimation has been standardized in which murine neuroblastoma cell line, Neuro-2A and tissue culture microtiter plates were used. Serum samples from 105 human beings, 30 canines, 7 rabbits and 4 monkeys were tested by RFFIT. Of these, 33 human and 20 canine sera were also tested by the mouse neutralization test (MNT).