Immobilization of keratinase on chitosan grafted-β-cyclodextrin for the improvement of the enzyme properties and application of free keratinase in the textile industry.

Immobilization of enzymes is an effective and potential technique for improving the enzyme characteristics and plays an important role in reducing the final cost of enzymatic reactions. However, the method of enzyme immobilization should be easy, cost-effective and environment friendly when applicable at industrial scale. In present study, the successful biochemical characterization of free and immobilized keratinase was evaluated.