Targeting the CLK2/SRSF9 splicing axis in prostate cancer leads to decreased ARV7 expression.

In advanced prostate cancer (PC), in particular after acquisition of resistance to androgen receptor (AR) signaling inhibitors (ARSI), upregulation of AR splice variants compromises endocrine therapy efficiency. Androgen receptor splice variant-7 (ARV7) is clinically the most relevant and has a distinct 3' untranslated region (3'UTR) compared to the AR full-length variant, suggesting a unique post-transcriptional regulation. Here, we set out to evaluate the applicability of the ARV7 3'UTR as a therapy target.

Anticancer dinuclear Ir(III) complex activates Nrf2 and interferes with NAD(H) in cancer cells.

Dinuclear complex [Ir(μ-L1)(η-Cp*)Cl](PF) (1) exhibits low micromolar cytotoxic activity in vitro in various human cancer cells (GI = 1.7-3.0 μM) and outperformed its mononuclear analogue [Ir(η-Cp*)Cl(L2)]PF (2; GI > 40.

Tools, techniques, methods, and processes for the detection and mitigation of fraudulent or erroneous data in evidence synthesis: a scoping review protocol.

Objective: This scoping review aims to identify, catalogue, and characterize previously reported tools, techniques, methods, and processes that have been recommended or used by evidence synthesizers to detect fraudulent or erroneous data and mitigate its impact.

Introduction: Decision-making for policy and practice should always be underpinned by the best available evidence-typically peer-reviewed scientific literature. Evidence synthesis literature should be collated and organized using the appropriate evidence synthesis methodology, best exemplified by the role systematic reviews play in evidence-based health care.

Assessing disease progression and treatment response in progressive multiple sclerosis.

Progressive multiple sclerosis poses a considerable challenge in the evaluation of disease progression and treatment response owing to its multifaceted pathophysiology. Traditional clinical measures such as the Expanded Disability Status Scale are limited in capturing the full scope of disease and treatment effects. Advanced imaging techniques, including MRI and PET scans, have emerged as valuable tools for the assessment of neurodegenerative processes, including the respective role of adaptive and innate immunity, detailed insights into brain and spinal cord atrophy, lesion dynamics and grey matter damage.

Exploring the benefits and challenges of AI-driven large language models in gastroenterology: Think out of the box.

Artificial Intelligence (AI) has evolved significantly over the past decades, from its early concepts in the 1950s to the present era of deep learning and natural language processing. Advanced large language models (LLMs), such as Chatbot Generative Pre-Trained Transformer (ChatGPT) is trained to generate human-like text responses. This technology has the potential to revolutionize various aspects of gastroenterology, including diagnosis, treatment, education, and decision-making support.

How to return? experiences of patients in working age after first Ischaemic stroke: an interpretative phenomenological analysis of patient´s perspective at 12 - 24 months post-stroke.

Background: Limited evidence of young adult patient-reported outcomes and experiences after ischaemic stroke has been conducted.

Aim: To investigate the meaning of the lived experiences of stroke patients in working age 12-24 months after their first IS.

Material And Methods: The exploratory qualitative study used an interpretative phenomenological analysis (IPA) design.

Where is the heat threat in a city? Different perspectives on people-oriented and remote sensing methods: The case of Prague.

Extreme heat in urban areas has a severe impact on urban populations worldwide. In light of the threats posed by climate change, it is clear that more holistic and people-oriented approaches to reducing heat stress in urban areas are needed. From this perspective we aim to identify and compare thermal hotspots and places with favourable thermal conditions, based on three different methods - thermal walk, participatory-based cognitive mapping, and remote sensing in a Central European city.

External Focus of Attention Reduces Cartilage Load During Drop Landings.

The aim of the present study was to examine the effects of attentional focus instructions on acute changes in the transverse relaxation time (T2) of the femorotibial cartilage and in cartilage volume during repeated drop-jump landings. Ten healthy females (M = 20.4 ± 0.

What to Choose for Estimating Leaf Water Status-Spectral Reflectance or In vivo Chlorophyll Fluorescence?

In the context of global climate change and the increasing need to study plant response to drought, there is a demand for easily, rapidly, and remotely measurable parameters that sensitively reflect leaf water status. Parameters with this potential include those derived from leaf spectral reflectance (R) and chlorophyll fluorescence. As each of these methods probes completely different leaf characteristics, their sensitivity to water loss may differ in different plant species and/or under different circumstances, making it difficult to choose the most appropriate method for estimating water status in a given situation.

Chocolate Ganaches: Formulation, Processing and Stability in View of the New Production Trends.

This review aims at the current trends in chocolate ganache production and recipe formulation. Ganache is a blend of chocolate, sugars, dairy, and other ingredients commonly used to fill pralines, pastries, etc. In spite of ganache's popularity in the food industry, a comprehensive review focused on the application of functional substances and ganache processing has not been discussed in the scientific literature.

Spider mite infestation triggers coordinated hormonal trade-offs enabling plant survival with a fitness cost.

Tetranychus urticae is an important pest that causes severe damage to a wide variety of plants and crops, leading to a substantial productivity loss. Previous research has been focused on plant defence response to T. urticae to improve plant resistance.

Daily state of motivation as the effect of appreciation in a diary study.

Background: Grounded in self-determination theory, the present study aimed to investigate whether daily changes in employee motivation depend on whether employees receive appreciation from various sources at work, using a 7-day diary design. Beyond general knowledge about the effects of appreciation as an important source of motivation, there is still a lack of knowledge about the intrapersonal effect of appreciation on different types of regulation/motivation in terms of self-determination theory over time.

Participants And Procedure: The sample consisted of 104 employees in full-time employment.

The revised JBI critical appraisal tool for the assessment of risk of bias for cohort studies.

Cohort studies are a robust analytical observational study design that explore the difference between two different cohorts on an outcome, differentiated by their exposure status. Despite being observational in nature, they are often included in systematic reviews of effectiveness, particularly when randomized controlled trials are limited or not feasible. Like all studies included in a systematic review, cohort studies must undergo a critical appraisal process to assess the extent to which a study has considered potential bias in its design, conduct, or analysis.

Frequency of benign tumors after partial nephrectomy and the association between malignant tumor findings and preoperative clinical parameters.

Background: Partial nephrectomy (PN) has become the dominant treatment modality for cT1 renal tumor lesions. Tumors suspected of malignant potential are indicated for surgery, but some are histologically classified as benign lesions after surgery. This study aims to analyze the number of benign findings after PN according to definitive histology and to evaluate whether there is an association between malignant tumor findings and individual factors.

Beyond glycan barriers: non-cognate ligands and protein mimicry approaches to elicit broadly neutralizing antibodies for HIV-1.

Human immunodeficiency virus type 1 (HIV-1) vaccine immunogens capable of inducing broadly neutralizing antibodies (bNAbs) remain obscure. HIV-1 evades immune responses through enormous diversity and hides its conserved vulnerable epitopes on the envelope glycoprotein (Env) by displaying an extensive immunodominant glycan shield. In elite HIV-1 viremic controllers, glycan-dependent bNAbs targeting conserved Env epitopes have been isolated and are utilized as vaccine design templates.

Getting a molecular grip on the half-lives of iminothioindoxyl photoswitches.

Visible-light-operated photoswitches are of growing interest in reversibly controlling molecular processes, enabling for example the precise spatiotemporal focusing of drug activity and manipulating the properties of materials. Therefore, many research efforts have been spent on seeking control over the (photo)physical properties of photoswitches, in particular the absorption maxima and the half-life. For photopharmacological applications, photoswitches should ideally be operated by visible light in at least one direction, and feature a metastable isomer with a half-life of 0.

Development of tungsten-modified iron oxides to decompose an over-the-counter painkiller, Acetaminophen by activating peroxymonosulfate.

Acetaminophen (APAP) is a well-known type of over-the-counter painkillers and is frequently found in surface waterbodies, causing hepatotoxicity and skin irritation. Due to its persistence and chronic effects on the environment, innovative solutions must be provided to decompose APAP, effectively. Innovative catalysts of tungsten-modified iron oxides (TF) were successfully developed via a combustion method and thoroughly characterized using SEM, TEM, XRD, XPS, a porosimetry analysis, Mössbauer spectroscopy, VSM magnetometry, and EPR.

Music Therapists' Job Satisfaction and Related Factors: A Cross-Sectional Survey in Five European Countries and Israel.

Studies on the job satisfaction of music therapists are needed for the growth of the profession and their findings may help to improve the well-being of practitioners. The aim of this cross-sectional survey was to explore the levels of satisfaction of music therapists with their profession and to identify the factors influencing their job satisfaction in several countries where this topic is under researched (Israel, Germany, Spain, Switzerland, Czech Republic, and Austria). Descriptive and inferential statistics of data obtained through an online survey of 439 practicing music therapists found the highest levels of job satisfaction were reported in Spain and the lowest levels were reported in Germany.

Phylogenetic structure of moth communities (Geometridae, Lepidoptera) along a complete rainforest elevational gradient in Papua New Guinea.

We use community phylogenetics to elucidate the community assembly mechanisms for Geometridae moths (Lepidoptera) collected along a complete rainforest elevational gradient (200-3700 m a.s.l) on Mount Wilhelm in Papua New Guinea.

Genotype is associated with left ventricular reverse remodelling and early events in recent-onset dilated cardiomyopathy.

Aims: Recent-onset dilated cardiomyopathy (RODCM) is characterized by heterogeneous aetiology and diverse clinical outcomes, with scarce data on genotype-phenotype correlates. Our aim was to correlate individual RODCM genotypes with left ventricular reverse remodelling (LVRR) and clinical outcomes.

Methods And Results: In this prospective study, a total of 386 Czech RODCM patients with symptom duration ≤6 months underwent genetic counselling and whole-exome sequencing (WES).

D-Hexopyranosides with Vicinal Nitrogen-Containing Functionalities.

Various substituted D-hexopypyranosides units with nitrogen-containing functionalities are present in many important natural compounds and pharmaceutical substances. Since their complex structural diversity contributes to a broad spectrum of biological functions and activities, these derivatives are frequently studied. This review covers syntheses of D-hexopyranosides with vicinal nitrogen-containing functionalities since the 1960s, when the first articles emerged.

GRADE guidance 39: using GRADE-ADOLOPMENT to adopt, adapt or create contextualized recommendations from source guidelines and evidence syntheses.

Background And Objective: The Grading of Recommendations, Assessment, Development and Evaluations (GRADE)-ADOLOPMENT methodology has been widely used to adopt, adapt, or de novo develop recommendations from existing or new guideline and evidence synthesis efforts. The objective of this guidance is to refine the operationalization for applying GRADE-ADOLOPMENT.

Methods: Through iterative discussions, online meetings, and email communications, the GRADE-ADOLOPMENT project group drafted the updated guidance.