871 results match your criteria: "Pakistan Institute of Engineering and Applied[Affiliation]"

Can nanotechnology and genomics innovations trigger agricultural revolution and sustainable development?

Funct Integr Genomics

November 2024

Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE- C, PIEAS), Faisalabad, 38000, Punjab, Pakistan.

At the dawn of new millennium, policy makers and researchers focused on sustainable agricultural growth, aiming for food security and enhanced food quality. Several emerging scientific innovations hold the promise to meet the future challenges. Nanotechnology presents a promising avenue to tackle the diverse challenges in agriculture.

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Article Synopsis
  • * GACI is a rare disease that results in significant arterial calcification, often leading to conditions like heart failure and pulmonary stenosis.
  • * A study identified a new pathogenic variant in ENPP1 from a Pakistani family, linked to severe valvular pulmonary stenosis, confirming its role in GACI through genetic analysis and functional studies.
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Omics-Driven Strategies for Developing Saline-Smart Lentils: A Comprehensive Review.

Int J Mol Sci

October 2024

School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.

A number of consequences of climate change, notably salinity, put global food security at risk by impacting the development and production of lentils. Salinity-induced stress alters lentil genetics, resulting in severe developmental issues and eventual phenotypic damage. Lentils have evolved sophisticated signaling networks to combat salinity stress.

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Weed control in chickpea (Cicer arietinum L.) is challenging due to narrow genetic base of available germplasm and limited herbicide options. In this view, present research was focused on induced mutagenesis in chickpea for development of herbicide (glyphosate) tolerant mutants and subsequent screening under field conditions.

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Cotton (Gossypium hirsutum L.) is a multipurpose crop. Abiotic stresses, especially extreme heat and drought, limit crop growth and thus reduce cotton yield by about 50%.

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Knockout mutation in enhances number of productive tillers in hexaploid wheat.

Front Genome Ed

October 2024

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan.

Article Synopsis
  • Recent advances in CRISPR-Cas technology enable precise genetic modifications in crops, particularly for improving wheat traits by targeting specific genes.
  • This study focuses on developing knockout mutant lines of an elite wheat cultivar, resulting in a significant increase in both tillers and grains per plant without affecting plant structure.
  • The method also utilizes co-transformation techniques to enhance regeneration efficiency of transgenic wheat, demonstrating the potential of CRISPR for boosting grain yield in elite cultivars for future agricultural improvements.
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(; Ranunculaceae), commonly referred to as black cumin, is one of the most widely used medicinal plants worldwide, with its seeds having numerous applications in the pharmaceutical and food industries. With the emergence of antibiotic resistance in pathogens as an important health challenge, the need for alternative microbe-inhibitory agents is on the rise, whereby black cumin has gained considerable attention from researchers for its strong antimicrobial characteristics owing to its high content in a wide range of bioactive compounds, including thymoquinone, nigellimine, nigellidine, quercetin, and O-cymene. Particularly, thymoquinone increases the levels of antioxidant enzymes that counter oxidative stress in the liver.

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Morpho-Molecular Identification of and Associated with Symptomatic Wilting of Potato from Pakistan.

J Fungi (Basel)

October 2024

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences, Faisalabad 38000, Pakistan.

Potato ( L.) is one of the emerging staple crops in Pakistan, with Punjab producing over 95% of the country's potatoes. Wilt is an emerging threat to the potato crop worldwide, including in Pakistan.

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Analyzing Antibiotic Resistance in Bacteria from Wastewater in Pakistan Using Whole-Genome Sequencing.

Antibiotics (Basel)

October 2024

Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.

Wastewater is a major source of Antibiotic-Resistant Bacteria (ARB) and a hotspot for the exchange of Antibiotic-Resistant Genes (ARGs). The occurrence of Carbapenem-Resistant Bacteria (CRB) in wastewater samples is a major public health concern. This study aimed to analyze Antibiotic resistance in bacteria from wastewater sources in Pakistan.

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Chickpeas are a highly versatile functional food legume that possesses the capacity to boost human health and has the potential to alleviate malnutrition-related deficiencies. To investigate whole seed-based nutritional and anti-nutritional composition, a set of 90 chickpea genotypes (66 desi and 24 kabuli) was collected from different research organizations in Pakistan. Significant variation (Tukey HSD test,  < 0.

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Ginger, a fresh rhizome, an economically important spice with extensive nutraceutical activities finds itself in vegetable and therapeutic market. Aflatoxins (AFB, AFB, AFG and AFG) along with ochratoxin A (OTA) are the most significant and the most toxic form of mycotoxins which are produced by various fungi. This study was initiated to assess the contamination of AFs and OTA in raw and dried ginger products, collected from different agro-climatic zones in Punjab, Pakistan employing the high performance liquid chromatography.

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Article Synopsis
  • Exposing wheat to physical mutagens generated new genetic resources that may help in managing various environmental stresses.
  • A high throughput GBS-DArTseq™ assay revealed significant genetic diversity among 33 stable wheat mutants, identifying over 1.5 million PAV markers distributed across different wheat chromosomes, with the highest counts found on Chr-7D and Chr-7B.
  • The study highlighted specific mutants with notable PAV counts linked to beneficial traits like disease resistance, enhanced photosynthesis, larger grain size, and improved yield, proving useful for molecular geneticists and breeders in developing resilient wheat varieties.
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  • The study evaluates the antibacterial effectiveness of Tryptophan-coordinated silver nanoparticles (Ag-TrpNPs) using conventional and synchronous fluorescence spectroscopy techniques.
  • Ag-TrpNPs are considered promising due to their antibacterial properties, but their potential toxicity necessitates coordination with biocompatible molecules like Tryptophan.
  • Results showed significant zones of inhibition for E. coli, confirming the Ag-TrpNPs' efficacy against bacteria at various concentrations, with optical characterization techniques confirming their size and morphology.
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Serum uric acid: an independent risk factor for cardiovascular disease in Pakistani Punjabi patients.

BMC Cardiovasc Disord

October 2024

Diabetes and Cardio-Metabolic Disorders Lab, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box. 577, Faisalabad, Pakistan.

Background: It is well-known that serum uric acid (SUA) can increase the risk of hypertension, diabetes, obesity and dyslipidemia. However, its independent association with the risk of cardiovascular diseases (CVD) is controversial particularly in different populations. Hence, this study was aimed to assess an independent association of SUA with CVD risk in a Punjabi Pakistani cohort.

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Data-driven classification and explainable-AI in the field of lung imaging.

Front Big Data

September 2024

PolitoBIOMed Lab, Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy.

Detecting lung diseases in medical images can be quite challenging for radiologists. In some cases, even experienced experts may struggle with accurately diagnosing chest diseases, leading to potential inaccuracies due to complex or unseen biomarkers. This review paper delves into various datasets and machine learning techniques employed in recent research for lung disease classification, focusing on pneumonia analysis using chest X-ray images.

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Nano-biochar is a source of blackish carbonaceous material, a prerequisite for sustainable crop productivity. By using a variety of feedstock materials, nanobiochar synthesis can be employed via pyrolysis. Therefore, a project was initiated to explore the morpho-physio-biochemical alteration at the vegetative stage of wheat crops after the foliar application of nanobiochar suspension (NBS).

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This study introduces a high-performance Ce-Co MOFs/TiCT nanocomposite, synthesized via hydrothermal methods, designed to advance supercapacitor technology. The integration of Ce-Co metal-organic frameworks (MOFs) with TiCT (Mxene) yields a composite that exhibits superior electrochemical properties. Structural analyses, including X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM), confirm the successful formation of the composite, featuring well-defined rod-like Ce-Co MOFs and layered TiCT sheets.

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Cotton (Gossypium hirsutum L.) is of great economic importance as a cultivated crop in many parts of the world. In addition to being a pillar of the textile industry, cotton and its byproducts are used for livestock feed, seed oil, and other products.

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The generation of laser pulses with controlled optical waveforms, and their measurement, lie at the heart of both time-domain and frequency-domain precision metrology. Here, we obtain mid-infrared waves via intra-pulse difference-frequency generation (IPDFG) driven by 16-femtosecond near-infrared pulses, and characterise the jitter of sub-cycle fractions of these waves relative to the gate pulses using electro-optic sampling (EOS). We demonstrate sub-attosecond temporal jitter at individual zero-crossings and sub-0.

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Drought is one of the foremost devastating abiotic stresses reported for rice crops. To improve the productivity of rice, diversity is being enlarged by induced mutation using a source of gamma rays. But this type of mutation rarely results in fruitful products because the chances of getting the desired mutant are very low.

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Background: Bovine leukocyte adhesion deficiency (BLAD), bovine citrullinemia (BC), and deficiency of Uridine monophosphate synthetase (DUMPS) are the common autosomal recessive disorders affecting the global dairy industry. BLAD leads to poor wound healing and recurrent infections. In BC, ammonia builds up leading to neurological disorders and death.

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Diabetes mellitus is a prevalent chronic disease necessitating timely identification for effective management. This paper introduces a reliable, straightforward, and efficient method for the minimally invasive identification of diabetes mellitus through nanosecond pulsed laser-induced breakdown spectroscopy (LIBS) by integrating a state-of-the-art machine learning approach. LIBS spectra were collected from urine samples of diabetic and healthy individuals.

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Soybean Glycine max L., paleopolyploid genome, poses challenges to its genetic improvement. However, the development of reference genome assemblies and genome sequencing has completely changed the field of soybean genomics, allowing for more accurate and successful breeding techniques as well as research.

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Identifying the physiological traits associated with DNA marker using genome wide association in wheat under heat stress.

Sci Rep

August 2024

Plant Breeding Institute, Sydney Institute of Agriculture, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.

Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress.

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