Real-World Efficacy and Safety of Oral Tofacitinib in Patients with Refractory Moderate to Severe Atopic Dermatitis: A Multicenter Retrospective Study.

Background: Atopic dermatitis (AD) has a complex etiology that includes Th2 polarization, which is accompanied by the cytokines IL4, IL-5, IL-13, and IL-31, as well as Th17 and Th22, and in chronic lesions, Th1 cells. Tofacitinib inhibits Th1-, Th2-, and Th17-associated cytokines by selectively blocking JAK1 and JAK3 receptors. We conducted a multicentric, retrospective chart analysis to study the efficacy and safety of tofacitinib in patients with moderate to severe refractory AD.

Platelet-Rich Plasma Has Better Long-Term Results Than Corticosteroids or Placebo for Chronic Plantar Fasciitis: Randomized Control Trial.

Plantar fasciitis is the most common cause of heel pain. Platelet-rich plasma (PRP) is a supersaturated concentration of autologous platelets that augments the natural healing response of fascia. Previous studies have shown the superiority of PRP over corticosteroids (CS) for chronic plantar fasciitis.

Expert Consensus on The Management of Dermatophytosis in India (ECTODERM India).

Background: Dermatophytosis management has become an important public health issue, with a large void in research in the area of disease pathophysiology and management. Current treatment recommendations appear to lose their relevance in the current clinical scenario. The objective of the current consensus was to provide an experience-driven approach regarding the diagnosis and management of tinea corporis, cruris and pedis.

Idiopathic gingival fibromatosis with asymmetrical presentation and electrosurgical management.

Idiopathic gingival fibromatosis is a rare genetically heterogeneous condition characterized by recurrent gingival enlargement without any identifiable cause. We report a case of 14-year-old female patient affected with sporadic, nonsyndromic, progressive gingival enlargement. It manifested more severely on the right side of the mouth with history of recurrence after prior conventional surgical excision.

Evolutionary growth of certain metabolic pathways involved in the functioning of GAD and INS genes in Type 1 Diabetes Mellitus: Their architecture and stability.

Background: Studying biochemical pathway evolution for diseases is a flourishing area of Systems Biology. Here, we study Type 1 Diabetes Mellitus (T1D), focusing on growth of glutamate, β-alanine, taurine and hypotaurine, and butanoate metabolisms involved in onset of GAD and INS genes in Homo sapiens with comparative analysis in non-obese diabetic Mus musculus, biobreeding Diabetes-prone Rattus norvegicus, Pan troglodytes, Oryctolagus cuniculus, Danio rerio and Drosophila melanogaster respectively.

Methods: We propose an algorithm for growth analysis for four metabolic pathways involved in T1D.

Characterization of Indian beers: chemical composition and antioxidant potential.

Chemical composition, antioxidant potential and corresponding lipid preoxidation of Indian commercial beers were evaluated. The presence of polyphenolic compounds such as tannic acid, gallic acid, catechol, vanillin, caffeic acid, quercetin, p-coumaric acid and rutin was quantified using LC-MS while the organic acids including tartaric, malic, acetic, citric and succinic acids were analysed using HPLC. Beer sample B8 had the greatest concentration of phenolic and flavonoid components (0.

Differential Expression of Antioxidant Enzymes During Degradation of Azo Dye Reactive black 8 in Hairy roots of Physalis minima L.

The enzymes involved in the protection of plant metabolism in presence of azo dye was characterized by studying activities of the role of antioxidant enzymes in the hairy roots (HRs) of Physalis minima L. during degradation of an azo dye, Reactive Black 8 (RB8). When the HRs were exposed to RB8 (30 mg L(-1)), a  nine fold increase in SOD activity was observed after 24 h, while 22 and 50 fold increase in activity was observed for POX and APX respectively after 72 h, whereas there was no significant change in activity of CAT.

Structure modeling and dynamics driven mutation and phosphorylation analysis of Beta-amyloid peptides.

The most common characteristics of diverse age-related neurodegenerative diseases are aggregation and accumulation of the misfolded protein in the brain. Alzheimer׳s disease (AD) is one of these protein conformational diseases. Extracellular accumulation of amyloid β (Aβ) is one the neuropathological hallmarks of Alzheimer disease.

Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC.

The aim of the present study was to evaluate the in vitro anti-cancer and anti-oxidant potential of methanol extract of Blumea eriantha as well as its phytochemical characterization. The in vitro cytotoxic and antiproliferative activities of B. eriantha methanolic extract of leaves were evaluated using MTT assay on HeLa and B16F10 cell lines, and wound scratch and colony formation assays on B16F10 cell lines.

α-Tocopherol mediated amelioration of camptothecin-induced free radical damage to avert cardiotoxicities.

Reactive oxygen species (ROS) such as O2(-), hydrogen peroxide, and OH(-) are highly toxic to cells. Cellular antioxidant enzymes and free radical scavengers normally protect a cell from toxic effects of ROS. However, when generation of ROS overtakes the antioxidant defense of the cells, it leads to various pathological conditions.

OLOGEN(®) implant in the management of glaucoma in an unusual case of Axenfeld-Rieger syndrome.

We report an unusual case of Axenfeld-Rieger Syndrome with secondary glaucoma managed with Ologen(®) implant. A 35-year-old male presented with complaints of decreased vision in both eyes of 22 years duration. His best corrected visual acuity (BCVA) was no perception of light in right eye and 20/30 in left eye.

Computer-aided drug designing.

Computer-aided drug designing has emerged as a cost-effective and rapid tool for the discovery of newer therapeutic agents. Several algorithms have been developed to analyze protein structure and function, to identify interacting ligands, active site residues, and to study protein-ligand interactions, which can eventually lead to the identification of new drugs. In silico drug designing involves identification of the target protein which is responsible for the development of the disease under study.

An In silico Based Comparison of Drug Interactions in Wild and Mutant Human β-tubulin through Docking Studies.

Background: Tubulin protein being the fundamental unit of microtubules is actively involved in cell division thus making them a potential anti-cancer drug target. In spite of many reported drugs against tubulin, few of them have started developing resistance in human β-tubulin due to amino acid substitutions.

Methods: In this study we generated three mutants (F270V, A364T and Q292E) using Modeller9v10 which were targeted with compounds from higher and lower plants along with marine isolates using iGEMDOCK2.

Comparative study of modified quantitative buffy coat and two rapid tests in comparison with peripheral blood smear in malaria diagnosis in mumbai, India.

In order to identify a quick and reliable technique for accurate diagnosis of malaria, study of the efficiency of the tests such as Parahit total (HRPII & aldolase Ag), Advantage mal card (parasite specific LDH), and modified QBC was done in comparison with conventional blood smear microscopy. One hundred patients infected with P. vivax and 101 infected with P.

A comparative study of karyomorphology among three populations of Garcinia indica (Clusiaceae) (Thomas-Dupetite) Choisy.

Garcinia indica is a tree species of the family Clusiaceae. This species is endemically distributed only in Ratnagiri and Sindhudurga district of Maharashtra. Plants collected in particularly Dive Agar and Sawantwadi show morphological variations.

Analyzing methods for path mining with applications in metabolomics.

Metabolomics is one of the key approaches of systems biology that consists of studying biochemical networks having a set of metabolites, enzymes, reactions and their interactions. As biological networks are very complex in nature, proper techniques and models need to be chosen for their better understanding and interpretation. One of the useful strategies in this regard is using path mining strategies and graph-theoretical approaches that help in building hypothetical models and perform quantitative analysis.

Simulating an infection growth model in certain healthy metabolic pathways of Homo sapiens for highlighting their role in Type I Diabetes mellitus using fire-spread strategy, feedbacks and sensitivities.

Disease Systems Biology is an area of life sciences, which is not very well understood to date. Analyzing infections and their spread in healthy metabolite networks can be one of the focussed areas in this regard. We have proposed a theory based on the classical forest fire model for analyzing the path of infection spread in healthy metabolic pathways.

HSP90: chaperone-me-not.

With increasing understanding of the molecular basis of carcinogenesis, its progression and metastasis, the cancer therapy has shifted from empirical approaches to targeting specific molecules that regulate the complex network of signalling pathways for cell survival and proliferation. These include key players in malignant transformation like protein kinases, transcription factors, steroid hormone receptors, cell cycle regulators, signal transduction proteins and regulators of apoptosis. Almost all these proteins depend upon the molecular chaperone Hsp90 for their proper folding, stability and function and thus are a part of the Hsp90 clientele.

Pattern of mandibular third molar impaction in the Indian population: a retrospective clinico-radiographic survey.

Objective: The objective of this study was to study incidence of type of impaction of mandibular third molars in the Indian population.

Study Design: Data of 1200 patients examined during a 5-year period were reviewed, which included status of mandibular third molar for inclination, emergence in oral cavity, and prevalence of caries. Radiographically, angulation of tooth, level of occlusal surface of third molar with respect to second molar, relationship between ascending ramus of mandible and distal surface of third molar, and proximity to inferior alveolar nerve were studied.

Cellunomics: the interaction analysis of cells.

Cellunomics envisions the new branch of cell, which integrates genomics and proteomics with a new knowledge base built from temporal and spatial data on the chemical and molecular interrelationships of cellular components, i.e., cellome.

Phylogenomics: evolution and genomics intersection.

Phylogenomics is the analysis of genomes of a group of closely related species. Almost all functional prediction methods rely on the identification, characterisation and quantification of sequence similarity between the gene of interest and genes for which functional information is available. This is the new evolved branch that is developed from the ongoing genome sequencing projects that have led to a phylogenetic approach based on genome-scale data.

microRNA: human disease and development.

microRNAs or miRNAs are an abundant class of highly conversed, small non-coding RNAs that present an entirely new theme of post-transcriptional gene regulation. miRNAs play a key role in diverse biological systems, such as virology, embryogenesis, differentiation, inflammation and cancer research. Research showed the importance of these non-coding small RNAs on immune system development and response.

Cytomics.

Cytomics is the branch of omics that takes into account the various bioinformatic techniques for understanding the functions and molecular architecture of the cytome. Cytomics, the multi-molecular cytometric analysis of the cellular heterogeneity of cytomes, access a maximum of information on the apparent molecular cell phenotype as it results from cell genotype and exposure. This has been done using various cytometrical procedures including microscopic techniques allowing the various components of a cell to be visualized as they interact in vivo.

Physiomics.

Physiomics is that branch of omics that uses large scale databases and experimental databases along with computer algorithms to study the physiological phenotypes of genes, proteins and their relationships. It deals with studying the physiome, the total integration of genome, proteome and metabolome, from cells to organisms. It is a very useful branch that has been actively used in studying drug development, various interactions and biosensor as well as biochip development.