Predicting breast cancer prognosis using PR and PIK3CA biomarkers: a comparative analysis of diagnostic groups.

Purpose: To evaluate the prognostic significance of progesterone receptor (PR) expression and the PIK3CA mutation status in HR+/HER2 - breast cancer patients, with the goal of screening patients who may derive the greatest benefit from PI3K-targeted therapy.

Methods: A retrospective analysis was conducted on 152 HR+/HER2 - breast cancer patients stratified by PR expression levels and PIK3CA mutation status. The study population was divided into groups on the basis of a median PR threshold of 50% and further subdivided by PIK3CA mutation status.

Rapid Preparation of Collagen/Red Blood Cell Membrane Tubes for Stenosis-Free Vascular Regeneration.

Extracellular matrix (ECM)-based small-diameter vascular grafts (SDVGs, inner diameter (ID) < 6 mm) hold great promise for clinical applications. However, existing ECM-based SDVGs suffer from limited donor availability, complex purification, high cost, and insufficient mechanical properties. SDVGs with ECM-like structure and function, and good mechanical properties were rapidly prepared by optimizing common materials and preparation, which can improve their clinical prospects.

Volatile Sieving Using Architecturally Designed Nanochannel Lamellar Membranes in Membrane Desalination.

Thermally driven membrane desalination processes have garnered significant interest for their potential in the treatment of hypersaline wastewater. However, achieving high rejection rates for volatiles while maintaining a high water flux remains a considerable challenge. Herein, we propose a thermo-osmosis-evaporation (TOE) system that utilizes molecular intercalation-regulated graphene oxide (GO) as the thermo-osmotic selective permeation layer, positioned on a hydrophobic poly(vinylidene fluoride) fibrous membrane serving as the thermo-evaporation layer.

Revolutionizing Heart Failure Therapy: Harnessing IVT mRNA and Fusion Protein Technology to Prolong rhBNP Half-Life.

Purpose: Recombinant human B-type natriuretic peptide (rhBNP) has been extensively proven to be an effective mean of heart failure (HF) therapy, but its clinical application is limited by its very short half-life. This study aims to combine in vitro transcribed mRNA (IVT mRNA) and fusion protein technology to develop a rhBNP-Fc mRNA drug with long half-life, high efficiency and few side effects to treat HF.

Methods: The rhBNP-Fc fusion mRNA with IgG4-Fc sequence was produced by IVT technology.

Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/G-quadruplex-based DNAzyme.

A self-powered dual-electrode aptasensor was developed for the detection of tumor marker carcinoembryonic antigen (CEA). The composite BiVO/ZnInS, which is capable of forming a Z-scheme heterojunction, was chosen as the photoanode, and the AuNP/CuBiO complex was chosen as the photocathode in photoelectrochemical (PEC) detection. The experiments showed that the constructed self-powered dual-electrode system had a good photoelectric response to white light, and the photocurrent signal of the photocathode was significantly enhanced under the influence of the photoanode.

Amplification-free sensitive detection of Staphylococcus aureus by spherical nucleic acid triggered CRISPR/Cas12a and Poly T-Cu reporter.

A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for Staphylococcus aureus (S. aureus) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the S.

Highly electroactive thiazolium [5,4-d]thiazol-2,5-dicarboxylic acid-silver electrochemiluminescent metal-organic frameworks: synthesis, properties and application in glutathione detection.

Thiazolo[5,4-d]thiazole-2,5-dicarboxylic acid (HThz), a thiazolothiazole (TTz) derivative with carboxylic acid groups, was synthesized as a ligand for the creation of five MOFs, each associated with distinct metal ions including Ag, Mn, Co, Zn, and Cu. The cathodic electrochemiluminescence (ECL) of HThz and the resulting MOFs was investigated. HThz was found to generate ECL signals, but this process was heavily reliant on potassium persulfate (KSO) as a co-reactant.

Accuracy (trueness and precision) of 3-dimensional virtual patients: an in vitro investigation of different facial scanners and digital integration techniques.

Objectives: To investigate the influence of different facial scanners and integration approaches on the accuracy of virtual dental patients (VDPs).

Methods: Forty VDPs were generated using a head mannequin and two facial scanners: 1) an industrial scanner and 2) a smartphone scanner. For each scanner, two integration methods were applied: 1) integration by virtual facebow scan and 2) integration by nose-teeth scan.

Cepharanthine relieves nonalcoholic steatohepatitis through inhibiting STAT1/CXCL10 axis-mediated lipogenesis and inflammatory responses.

Ethnopharmacological Relevance: Stephania rotunda Lour., a medicinal herb, has been utilized in both Traditional Chinese Medicine (TCM) and Traditional Indian Medicine to treat conditions such as fever, dysentery, and inflammation. Cepharanthine (CEP), a primary active ingredient of Stephania rotunda Lour.

The Emerging Role of Mitochondria Oxygen Redox in Pathological Progression of Neurodegenerative Disorders.

Neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, and Huntington disease, pose serious threats to human health, leading to substantial economic burdens on society and families. Despite extensive research, the underlying mechanisms driving these diseases remain incompletely understood, impeding effective diagnosis and treatment. In recent years, growing evidence has highlighted the crucial role of oxidative stress in the pathogenesis of various neurodegenerative diseases.

Microneedle drug delivery system based on hyaluronic acid for improving therapeutic efficiency of hypertrophic scars.

Hypertrophic scar (HS) is a disease with excessive skin fibrosis and collagen disorder, which is generally caused by abnormal wound repair process after burn and trauma. Although intralesional injection of 5-fluorouracil (5-Fu) has been used in clinical treatment of HS, the patients' compliance of injection treatment is poor. In this study, a double-layer dissolution microneedle (MN) containing asiaticoside (AS) and 5-Fu was designed for the treatment of HS.

High efficiency adsorption of boron by sodium alginate/polyethyleneimine/polysiloxane composite aerogel.

In this work, a new biomass boron adsorbent of N-methyl-D-glucosamine embedded sodium alginate/polyethyleneimine/polysiloxane composite aerogel (SKPN) was reported. Relevant characterization proved that the aerogel exhibited 3D porous structure with plenty of hydroxyl and amino functional groups, which was beneficial to the diffusion of boron and the chelation between boron and SKPN. Various parameters affecting the adsorption performance including pH value, contact time initial concentration, temperature and reusability were investigated.

Microwave-assisted extraction of dandelion root polysaccharides: Extraction process optimization, purification, structural characterization, and analysis of antioxidant activity.

This study aimed to establish a microwave-assisted method (MAE) for the efficient extraction of polysaccharides from dandelion roots. This study investigated the molecular structure and bioactivity of the polysaccharides from dandelion roots. Extraction conditions were optimized using response surface methodology (RSM).

Neoadjuvant chemoimmunotherapy for locally advanced squamous cell carcinoma of the head and neck: Systematic review and meta-analysis.

Background: Neoadjuvant chemoimmunotherapy emerged as a promising treatment for locally advanced squamous cell carcinoma of the head and neck (LA-SCCHN). However, a comparison of clinical outcomes with neoadjuvant chemotherapy was lacking.

Objective: To provide evidence supporting clinical decision-making for neoadjuvant chemoimmunotherapy in LA-SCCHN treatment.

Unraveling the tumor microenvironment of esophageal squamous cell carcinoma through single-cell sequencing: A comprehensive review.

Esophageal squamous cell carcinoma (ESCC) is a highly heterogeneous and aggressive malignancy. The progression, invasiveness, and metastatic potential of ESCC are shaped by a multitude of cells within the tumor microenvironment (TME), including tumor cells, immune cells, endothelial cells, as well as fibroblasts and other cell types. Recent advancements in single-cell sequencing technologies have significantly enhanced our comprehension of the diverse landscape of ESCC.

Chiral alkynyl Au(I) complexes: Enhancing chiroptical amplification of circularly polarized luminescence through supramolecular helices.

The construction of helical structures through self-assembly and the exploration of their formation mechanisms not only amplify chiroptical properties but also provide profound insights into the structures and functions of natural helices. In this study, we developed a chiral Au(I) system based on BINAP and alkynyl ligands. The modification of the length or number of alkyl chains at the terminal positions of the alkynyl ligands significantly impacted the self-assembly behavior of the complexes.

P2-NaMnNiCoO stabilized by optimal active facets for sodium-ion batteries.

Considering factors such as crustal reserves, atomic mass, redox potential and energy density, sodium-ion batteries (SIBs) are regarded as the most promising alternative to lithium-ion batteries (LIBs). Transition metal-based layered oxides, especially typical NaMnO, stand out among cathode materials due to their low cost and high energy density. However, NaMnO cathodes face several challenges, including Jahn-Teller distortion, manganese dissolution, structural collapse, irreversible phase transition and significant capacity loss.

Lateral flow assay with automatic signal amplification based on delayed substrate release.

The low sensitivity of Lateral flow assay (LFA) limits its application in rapid detection for trace targets. LFAs with nanozyme (nanozyme-LFA) as signal labels have demonstrated excellent performance in point of care testing (POCT). However, additional operational steps for substrate catalysis in nanozyme LFA are required, which makes the nanozyme-LFA operation complicated.