Design of magnetic polyethyleneimine-fluorescein isothiocyanate composites toward efficient enrichment of phosphopeptides.

Phosphoproteins maintain the normal metabolic activity of the organisms. Direct phosphopeptides detection is difficult to be realized by mass spectroscopy (MS) due to the low ionization efficiency, low abundance of phosphopeptides and interferences of complicated biological fluids. In the present work, a magnetic composite material was prepared by combining polyethyleneimine (PEI) and fluorescein isothiocyanate (FITC) focusing on phosphopeptides enrichment.

Validation of the 9th edition of the TNM staging system for limited-stage small cell lung cancer after Resection: A multicenter study.

Objectives: The 9th edition of the tumor-node-metastasis (TNM) staging system for lung cancer was proposed at the 2023 World Conference on Lung Cancer in Singapore. This study aimed to externally validate and compare the latest staging of small-cell lung cancer (SCLC).

Methods: Four hundred and eight patients with limited-stage SCLC were collected after lung resection from four centers.

TRIM44 alleviates renal ischemia-reperfusion injury by inhibiting pyroptosis through the NLRP3 pathway.

Background: Renal ischemia-reperfusion injury (IRI) is a prevailing manifestation of acute kidney injury (AKI) with limited treatment options. TRIM44 has emerged as a possible target for treatment due to its regulatory function in inflammatory pathways.

Methods: In vivo and in vitro models were employed to ascertain the TRIM44 impact on renal IRI.

Widely targeted metabolomics analysis reveals dynamic changes in metabolites of 'Hass' avocado during postharvest ripening period.

Avocado (Persea americana Mill) is a climacteric fruit harvested at the green stage that ripens postharvest to become edible. The 'Hass' cultivar, known for its rich, buttery flavor and pear-shaped appearance, was studied for metabolite changes during postharvest ripening using UPLC-MS/MS approach. The 16-day ripening period could be divided into three stages: evolution (days 1-9), edible ripe (days 10-15) and overripe (day 16).

Modeling and control of a sperm-inspired robot with helical propulsion.

Efficient propulsion has been a central focus of research in the field of biomimetic underwater vehicles. Compared to the prevalent fish-like reciprocating flapping propulsion mode, the sperm-like helical propulsion mode features higher efficiency and superior performance in high-viscosity environments. Based on the previously developed sperm-inspired robot, this paper focuses on its dynamic modeling and depth control research.

Tuning the spin state of the iron center by FePc/Mg(OH) heterojunction boosting oxygen reduction performance.

Iron phthalocyanine (FePc) is a promising non-noble metal catalyst for oxygen reduction reaction (ORR). While, with the plane-symmetric FeN site, the ORR activity of FePc is generally low due to its low ability to adsorb and activate O. Herein, we anchor FePc on Mg(OH)/N-doped carbon nanosheets building the ternary plate-like catalyst FePc/Mg(OH)/NC.

Bamboo fiber-derived carbon support for the immobilization of Pt nanoparticles to enhance hydrogen evolution reaction.

Biomass-derived carbon, as an excellent support, has received extensive attention. In this work, carbon matrix obtained from bamboo fiber (BF) is served as a supporting material for the immobilization of platinum (Pt) nanoparticles, leading to a substantial improvement in the hydrogen evolution reaction (HER). This approach leverages the remarkable surface area, outstanding conductivity, and environmentally friendly characteristics of BF-derived carbon, facilitating the dispersion and stability of the Pt nanoparticles.

Tunable architecture of cobalt-nickel metal-organic framework/activated carbon composites for superior electrochemical performance in asymmetric supercapacitors.

Cobalt-nickel metal-organic framework/activated carbon (MOF/AC) composites with tunable flower-like architectures were synthesized via a straightforward hydrothermal method, utilizing activated carbon as a structural and functional modifier. This modification increased the surface area from 20.3 m/g to 164.

Bilayer Mn-based Prussian blue cathode with high redox activity for boosting stable cycling in aqueous sodium-ion half/full batteries.

The Mn-based Prussian blue analogs (PBAs) have garnered significant attention due to their high specific capacity, stemming from the unique multi-electron reactions with Na. However, the structural instability caused by multi-ion insertion impacts the cycle life, thus limiting their further application in aqueous sodium-ion batteries (ASIBs). To address this issue, this work employed an in situ epitaxial solvent deposition method to homogeneously grow Ni hexacyanoferrate (NiHCF) on the surface of MnPBA, which can effectively overcome the de-intercalation instability.

Renal cell carcinoma with ALK-TPM3 gene fusion and ALK amplification: A case report and literature review.

Background: Anaplastic lymphoma kinase (ALK)-rearranged renal cell carcinoma (ALK-RCC) is a rare molecularly defined tumor entity included in the fifth edition of the World Health Organization Classification of Tumors. It is characterized by rearrangement of the ALK gene with various fusion partner genes, which most commonly results in oncogenic fusion proteins leading to ALK activation.

Case Presentation: A 30-year-old Chinese man underwent partial nephrectomy for a left renal tumor measuring 5 cm in diameter.

Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis.

Male osteoporosis is primarily caused by a decrease in testicular testosterone production. Male osteoporosis remains a disease with insufficient diagnosis and treatment, and its consequences are severe, especially in older patients. The gut microbiota plays a crucial role in its occurrence and development.

PIM1 enhances insulin secretion and inhibits ferroptosis of high glucose-induced pancreatic β-cells through strengthening PINK1/Parkin-mediated mitophagy via inactivating JNK/p38 signaling pathway.

Background: Diabetes mellitus (DM), a chronic metabolic disease, is characterized by long-term hyperglycemia resulting from the defect of insulin production and insulin resistance. The damage and dysfunction of pancreatic β-cells is a main link in DM development.

Methods: In this work, pancreatic β-cell line INS-1E cells were exposed to 30 mM glucose for 48 h to construct an in vitro DM model.

Procyanidin-crosslinked gradient silk fibroin composite nanofiber scaffold with sandwich structure for rotator cuff repair.

Improving the regeneration of the tendon-bone interface (TBI) helps to decrease the risk of rotator cuff retears after repair surgeries. Unfortunately, the lack of inherent healing capacity of the TBI, insufficient mechanical properties, and abnormal and persistent inflammation during repair are the key factors leading to suboptimal healing of the rotator cuff. Therefore, a high-strength rotator cuff repair material capable of regulating the unbalanced immune response and enhancing the regeneration of the TBI is urgently needed.

Robust and Regular Micronano Binary Texture on the Complex Curved Surface for Enhanced Reendothelialization and Antithrombotic Performance.

Blood-contacting medical devices can easily trigger immune responses, leading to thrombosis and hyperblastosis. Constructing microtexture that provides efficient antithrombotic and rapid reendothelialization performance on complex curved surfaces remains a pressing challenge. In this work, we present a robust and regular micronano binary texture on the titanium surface, characterized by exceptional mechanical strength and precisely controlled wettability to achieve excellent hemocompatibility.

ALC-0315 Lipid-Based mRNA LNP Induces Stronger Cellular Immune Responses Postvaccination.

At the end of 2019, SARS-CoV-2 emerged and rapidly spread, having a profound negative impact on human health and socioeconomic conditions. In response to this unprecedented global health crisis, significant advancements were made in the mRNA vaccine technology. In this study, we have compared the difference between two SARS-CoV-2 receptor-binding domain (RBD) mRNA-Lipid nanoparticle (LNP) vaccines prepared from two different ionizable cationic lipids: ALC-0315 and MC3.

Survival with Trastuzumab Emtansine in Residual HER2-Positive Breast Cancer.

Background: Patients with human epidermal growth factor receptor 2 (HER2)-positive early breast cancer with residual invasive disease after neoadjuvant systemic therapy have a high risk of recurrence and death. The primary analysis of KATHERINE, a phase 3, open-label trial, showed that the risk of invasive breast cancer or death was 50% lower with adjuvant trastuzumab emtansine (T-DM1) than with trastuzumab alone.

Methods: We randomly assigned patients with HER2-positive early breast cancer with residual invasive disease in the breast or axilla after neoadjuvant systemic treatment with taxane-based chemotherapy and trastuzumab to receive T-DM1 or trastuzumab for 14 cycles.

Anchoring of Probiotic-Membrane Vesicles in Hydrogels Facilitates Wound Vascularization.

Inadequate vascularization significantly hampers wound recovery by limiting nutrient delivery. To address this challenge, we extracted membrane vesicles from (LMVs) and identified their angiogenic potential via transcriptomic analysis. We further developed a composite hydrogel system (Gel-LMVs) by anchoring LMVs within carboxylated chitosan and cross-linking it with oxidized hyaluronic acid through a Schiff base reaction.

α-Actinin-1 deficiency in megakaryocytes causes low platelet count, platelet dysfunction, and mitochondrial impairment.

Cytoskeletal remodeling and mitochondrial bioenergetics play important roles in thrombocytopoiesis and platelet function. Recently, α-actinin-1 mutations have been reported in patients with congenital macrothrombocytopenia. However, the role and underlying mechanism of α-actinin-1 in thrombocytopoiesis and platelet function remain elusive.

Design, Synthesis, and Imaging of a Stable Xanthene-Based Dye with NIR-II Emission up to 1450 nm.

The development of long-wavelength near-infrared II (NIR-II, 900-1700 nm) dyes is highly desirable but challenging. To achieve both red-shifted absorption/emission and superior imaging capabilities, a donor-acceptor-donor (D-A-D) xanthene core was strategically modified by extending π-conjugated double bonds and enhancing electron-donating properties. Two dyes named and were synthesized and exhibited notably red-shifted absorption/emission peaks at 942/1250 and 1098/1450 nm, respectively.

ThCTi@(6)-C: Th═C Double Bond in a Mixed Actinide-Transition Metal Cluster.

A thorium-carbon double bond that corresponds to the sum of theoretical covalent double bond radii has long been sought after in the study of actinide-ligand multiple bonding as a synthetic target. However, the stabilization of this chemical bond remains a great challenge to date, in part because of a relatively poor energetic matching between 5f-/6d- orbitals of thorium and the 2s-/2p- frontier orbitals of carbon. Herein, we report the successful synthesis of a thorium-carbon double bond in a carbon-bridged actinide-transition metal cluster, i.

Comparison the Cosmetic Outcome of the Triangular Flap Insertion Versus Direct Linear Resection in Correction of Dog Ear Deformities on the Face-A Prospective and Randomized Controlled Trial.

Background: Compared with direct linear resection, the triangular flap insertion method is a correction method that purportedly reduces the incision tension of dog ears deformity. Randomized clinical trials comparing these 2 methods seem to be limited or absent.

Objective: A randomized study was planned to compare the cosmetic effect and scar in the defect area between the triangular flap insertion method and direct linear resection in the repair of dog ear deformities after the suture of the facial circular defect.

A Multifunctional Synergistic Solar-Driven Interfacial Evaporator for Desalination and Photocatalytic Degradation.

The scarcity of freshwater resources and the treatment of dye wastewater have emerged as unavoidable challenges that need to be addressed. The combination of solar-driven interfacial evaporation, photocatalytic degradation, and superhydrophobic surface provides an effective approach for seawater desalination and the treatment of organic dyes. In this study, we fabricated a multifunctional synergistic solar evaporator by depositing cupric oxide nanoparticles onto polypyrrole (PPy) coating and subsequently modified it with a hydrophobic agent successfully.

Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes.

Separation of polar compounds especially with similar polarities is challenging. In the present study, three polar compounds with similar polarities, including gentiopicroside, sweroside and mangiferin, have been successfully separated from Swertia mussotii by a combination of two counter-current chromatography modes. With the selected solvent system of ethyl acetate/n-butanol/water (8/2/10, v/v), a continuous injection mode was firstly employed.

Dimensional engineering of interlayer for efficient large-area perovskite solar cells with high stability under ISOS-L-3 aging test.

The utilization of low-dimensional perovskites (LDPs) as interlayers on three-dimensional (3D) perovskites has been regarded as an efficient strategy to enhance the performance of perovskite solar cells. Yet, the formation mechanism of LDPs and their impacts on the device performance remain elusive. Herein, we use dimensional engineering to facilitate the controllable growth of 1D and 2D structures on 3D perovskites.

Siderophore synthetase-receptor gene coevolution reveals habitat- and pathogen-specific bacterial iron interaction networks.

Bacterial social interactions play crucial roles in various ecological, medical, and biotechnological contexts. However, predicting these interactions from genome sequences is notoriously difficult. Here, we developed bioinformatic tools to predict whether secreted iron-scavenging siderophores stimulate or inhibit the growth of community members.