Prediction of p-phenylenediamine antioxidant concentrations in human urine using machine learning models.

p-phenylenediamine antioxidants (PPDs) are extensively used in rubber manufacturing for their potent antioxidative properties, but PPDs and 2-anilino-5-[(4-methylpentan-2yl)amino]cyclohexa-2,5-diene-1,4-dione (6PPDQ) pose potential environmental and health risks. Existing biomonitoring methods for assessing human exposure to PPDs are labor-intensive, costly, and provide limited data. Thus, there is a critical need to develop predictive models for evaluating PPDs and 6PPDQ exposure levels to facilitate health risk assessments.

Establishment of a novel large-scale targeted metabolomics method based on NFSWI-DDA mode utilizing HRMS and TQ-MS.

Metabolites identification is the major bottleneck in untargeted LC-MS metabolomics, primarily due to the limited availability of MS information for most detected metabolites in data dependent acquisition (DDA) mode. To solve this problem, we have integrated the iterative, interval, and segmented window acquisition concepts to develop an innovative non-fixed segmented window interval data dependency acquisition (NFSWI-DDA) mode, which achieves comparable MS coverage to data independent acquisition (DIA) mode. This acquisition strategy harnesses the strengths of both DDA and DIA, which could provide extensive coverage and excellent reproducibility of MS spectra.

Design of magnetic polyethyleneimine-fluorescein isothiocyanate composites toward efficient enrichment of phosphopeptides.

Phosphoproteins maintain the normal metabolic activity of the organisms. Direct phosphopeptides detection is difficult to be realized by mass spectroscopy (MS) due to the low ionization efficiency, low abundance of phosphopeptides and interferences of complicated biological fluids. In the present work, a magnetic composite material was prepared by combining polyethyleneimine (PEI) and fluorescein isothiocyanate (FITC) focusing on phosphopeptides enrichment.

Strong coupling FeVO nanoparticles/3D N-doped interconnected porous carbon derived from MOFs by confined adsorption-assembly-pyrolysis for greatly boosting oxygen reduction.

Low-cost and effective electrocatalysts are critical for energy storage and conversion. Herein, iron(III) and vanadium(III) acetylacetonates were first adsorbed and confined in porous zeolitic imidazolate framework-8 (ZIF-8), which further cross-linked together by the methanol-induced-assembly. Following the pyrolysis, the FeVO nanoparticles were efficiently encapsulated within three-dimensional (3D) N-doped interconnected porous carbon, termed FeVO/NIPC.

Chicken adipose tissue is differentially involved in primary and secondary regional immune response to NDV through miR-20a-5p-NR4A3 pathway.

The mammalian adipose tissue (AT) plays a key role in regulating immune function and anti-infective protection to maintain tissue regional homeostasis. However, it is still unclear whether there are differences in the participation of AT in primary and secondary immune response, and whether avian AT has the similar immune function characteristics to mammals. In this study, we used Newcastle disease virus (NDV) attenuated vaccine to induce primary and secondary immune response in chickens, and the changes of the key regulatory gene NR4A3 (nuclear receptor subfamily 4 group A member 3) of T cells activation and its targeted miR-20a-5p were detected by quantitative real-time PCR (qRT-PCR).

Establishment and application of a sandwich ELISA method for measuring Toxoplasma gondii circulating fructose-1,6-bisphosphate aldolase (ALD) protein in cats.

Toxoplasmosis is an important public health concern. Cats play a crucial role in increasing the risk of toxoplasmosis transmission to humans. Early diagnosis in cats is essential for the prevention and control of toxoplasmosis.

Validation of the 9th edition of the TNM staging system for limited-stage small cell lung cancer after Resection: A multicenter study.

Objectives: The 9th edition of the tumor-node-metastasis (TNM) staging system for lung cancer was proposed at the 2023 World Conference on Lung Cancer in Singapore. This study aimed to externally validate and compare the latest staging of small-cell lung cancer (SCLC).

Methods: Four hundred and eight patients with limited-stage SCLC were collected after lung resection from four centers.

A novel and quick egg yolk immunoglobulin y antibody extraction method leveraging the protein liquid-liquid phase separation principle.

This study presents a novel and efficient method for extracting immunoglobulin Y (IgY) antibodies from egg yolk based on the principle of liquid-liquid phase separation (LLPS) induced by polyethylene glycol 8000 (PEG 8000). Initial delipidation of egg yolk samples with varying PEG 8000 concentrations demonstrated optimal delipidation efficiency and protein recovery at 2.5 % PEG 8000 concentration.

TRIM44 alleviates renal ischemia-reperfusion injury by inhibiting pyroptosis through the NLRP3 pathway.

Background: Renal ischemia-reperfusion injury (IRI) is a prevailing manifestation of acute kidney injury (AKI) with limited treatment options. TRIM44 has emerged as a possible target for treatment due to its regulatory function in inflammatory pathways.

Methods: In vivo and in vitro models were employed to ascertain the TRIM44 impact on renal IRI.

Oral administration of Folium Artemisiae Argyi-derived exosome-like nanovesicles can improve ulcerative colitis by regulating intestinal microorganisms.

Background: Ulcerative colitis (UC), an inflammatory disease characterized by intestinal barrier dysfunction, poses significant challenges because of the toxicity and adverse effects commonly associated with conventional therapies. Safer and more efficacious treatment strategies are needed.

Purpose: The purpose of this study was to treat UC with Folium Artemisiae Argyi exosome-like nanovesicles (FAELNs) and to explore its related mechanism to provide a safer and more effective means for the treatment of ulcerative colitis.

Widely targeted metabolomics analysis reveals dynamic changes in metabolites of 'Hass' avocado during postharvest ripening period.

Avocado (Persea americana Mill) is a climacteric fruit harvested at the green stage that ripens postharvest to become edible. The 'Hass' cultivar, known for its rich, buttery flavor and pear-shaped appearance, was studied for metabolite changes during postharvest ripening using UPLC-MS/MS approach. The 16-day ripening period could be divided into three stages: evolution (days 1-9), edible ripe (days 10-15) and overripe (day 16).

Modeling and control of a sperm-inspired robot with helical propulsion.

Efficient propulsion has been a central focus of research in the field of biomimetic underwater vehicles. Compared to the prevalent fish-like reciprocating flapping propulsion mode, the sperm-like helical propulsion mode features higher efficiency and superior performance in high-viscosity environments. Based on the previously developed sperm-inspired robot, this paper focuses on its dynamic modeling and depth control research.

Association between maternal serum zinc and birth weight is modified by neonatal SOD2 polymorphism and promoter methylation.

Background: Conflicting findings exist regarding the association between maternal serum zinc and neonatal birth weight. This study aimed to explore the association between maternal serum zinc and birth weight, and whether this association was modified by neonatal SOD2 polymorphism and promoter methylation.

Methods: We recruited 464 mother-newborn pairs at Houzhai Center Hospital from January 2010 to January 2012.

Advancements in iron-based photocatalytic degradation for antibiotics and dyes.

The accelerated growth of the economy and advancements in medical technology have led to the discharge of a diverse range of organic pollutants into water sources. Recent investigations into water treatment have demonstrated the potential for integrating photocatalysis with techniques such as photocatalytic persulfate activation and the Photo-Fenton process for more efficient wastewater management. Iron-based photocatalysts responsive to visible light offer several advantages, including non-toxicity, safety, affordability, and excellent chemical and optical properties.

A Thermally Robust Biopolymeric Separator Conveys K Transport and Interfacial Chemistry for Longevous Potassium Metal Batteries.

Potassium metal batteries (KMBs) hold promise for stationary energy storage with certain cost and resource merits. Nevertheless, their practicability is greatly handicapped by dendrite-related anodes, and the target design of specialized separators to boost anode safety is in its nascent stage. Here, we develop a thermally robust biopolymeric separator customized via a solvent-exchange and amino-siloxane decoration strategy to render durable and safe KMBs.

Tuning the spin state of the iron center by FePc/Mg(OH) heterojunction boosting oxygen reduction performance.

Iron phthalocyanine (FePc) is a promising non-noble metal catalyst for oxygen reduction reaction (ORR). While, with the plane-symmetric FeN site, the ORR activity of FePc is generally low due to its low ability to adsorb and activate O. Herein, we anchor FePc on Mg(OH)/N-doped carbon nanosheets building the ternary plate-like catalyst FePc/Mg(OH)/NC.

Bamboo fiber-derived carbon support for the immobilization of Pt nanoparticles to enhance hydrogen evolution reaction.

Biomass-derived carbon, as an excellent support, has received extensive attention. In this work, carbon matrix obtained from bamboo fiber (BF) is served as a supporting material for the immobilization of platinum (Pt) nanoparticles, leading to a substantial improvement in the hydrogen evolution reaction (HER). This approach leverages the remarkable surface area, outstanding conductivity, and environmentally friendly characteristics of BF-derived carbon, facilitating the dispersion and stability of the Pt nanoparticles.

Tunable architecture of cobalt-nickel metal-organic framework/activated carbon composites for superior electrochemical performance in asymmetric supercapacitors.

Cobalt-nickel metal-organic framework/activated carbon (MOF/AC) composites with tunable flower-like architectures were synthesized via a straightforward hydrothermal method, utilizing activated carbon as a structural and functional modifier. This modification increased the surface area from 20.3 m/g to 164.

Bilayer Mn-based Prussian blue cathode with high redox activity for boosting stable cycling in aqueous sodium-ion half/full batteries.

The Mn-based Prussian blue analogs (PBAs) have garnered significant attention due to their high specific capacity, stemming from the unique multi-electron reactions with Na. However, the structural instability caused by multi-ion insertion impacts the cycle life, thus limiting their further application in aqueous sodium-ion batteries (ASIBs). To address this issue, this work employed an in situ epitaxial solvent deposition method to homogeneously grow Ni hexacyanoferrate (NiHCF) on the surface of MnPBA, which can effectively overcome the de-intercalation instability.

Renal cell carcinoma with ALK-TPM3 gene fusion and ALK amplification: A case report and literature review.

Background: Anaplastic lymphoma kinase (ALK)-rearranged renal cell carcinoma (ALK-RCC) is a rare molecularly defined tumor entity included in the fifth edition of the World Health Organization Classification of Tumors. It is characterized by rearrangement of the ALK gene with various fusion partner genes, which most commonly results in oncogenic fusion proteins leading to ALK activation.

Case Presentation: A 30-year-old Chinese man underwent partial nephrectomy for a left renal tumor measuring 5 cm in diameter.

Gastric duplication cysts with mixed hemangioma treated by endoscopic submucosal dissection: A case report and literature review.

Gastric duplication cysts (GDCs) are rare cystic neoplasms that are often difficult to distinguish from other entities. Accurate diagnosis of cysts before resection is difficult even using the most advanced imaging techniques. We present a case of a gastric duplication cyst in a 17-year-old female, presenting with discomfort in the upper abdomen.

Proteomics-on-a-Chip - Microfluidics meets proteomics.

Proteomics provides an understanding of biological systems by enabling the detailed study of protein expression profiles, which is crucial for early disease diagnosis. Microfluidic-based proteomics enhances this field by integrating complex proteome analysis into compact and efficient systems. This review focuses on developing microfluidic chip structures for proteomics, covering on-chip sample pretreatment, protein extraction, purification, and identification in recent years.

Enhanced piezoelectric sensor to distinguish real-time arrhythmia for predicting heart failure.

Monitoring cardiac rhythm is crucial for diagnosis of heart failure. However, the deficient sensitivity of polyvinylidene fluoride (PVDF) sensors impede their application in monitoring of cardiac rhythm due to the limited piezoelectricity. Here, doping of CoFeO and aligning fibers were jointly adopted to enhance the piezoelectricity of PVDF, attributed to the transformation of α-PVDF to β-PVDF from 51.

Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis.

Male osteoporosis is primarily caused by a decrease in testicular testosterone production. Male osteoporosis remains a disease with insufficient diagnosis and treatment, and its consequences are severe, especially in older patients. The gut microbiota plays a crucial role in its occurrence and development.

PIM1 enhances insulin secretion and inhibits ferroptosis of high glucose-induced pancreatic β-cells through strengthening PINK1/Parkin-mediated mitophagy via inactivating JNK/p38 signaling pathway.

Background: Diabetes mellitus (DM), a chronic metabolic disease, is characterized by long-term hyperglycemia resulting from the defect of insulin production and insulin resistance. The damage and dysfunction of pancreatic β-cells is a main link in DM development.

Methods: In this work, pancreatic β-cell line INS-1E cells were exposed to 30 mM glucose for 48 h to construct an in vitro DM model.