Unlocking Green Biomanufacturing Potential: Superior Heterologous Gene Expression with a T7 Integration Overexpression System in .

Industrial biotechnology employs cells for producing valuable products and serving as biocatalysts sustainably, addressing resource, energy, and environmental issues. is a preferred host for creating microbial chassis cells and producing industrial enzymes and functional nutritional products. In this study, a dual-module T7 integration expression system in was established.

Phthalimidine Synthesis via Iridium-Catalyzed Reductive Lactamization.

Herein, we report a sustainable and efficient method for the synthesis of structurally diverse phthalimidines from 2-formylbenzoic acid and primary amines using an iridium-catalyzed reductive lactamization strategy. The advantages of this method, such as the use of water-ethanol as a solvent, broad substrate scope, high catalyst efficiency (/ up to 10000), good scalability, and easy purification, enable it to be a practical approach to phthalimidines. It is suggested that iridium hydride formation is involved in the rate-limiting step.

Ectopic endometrial stromal cell-derived extracellular vesicles encapsulating microRNA-25-3p induce endometrial collagen I deposition impairing decidualization in endometriosis.

Endometrial collagen I undergoes dynamic degradation and remodelling in response to endometrial stromal cell (ESC) decidualization and embryo implantation. However, excessive collagen I deposition in the endometrium during the implantation window may impair decidualization, causing embryo implantation failure in patients with endometriosis (EMS). We found that endometrial collagen I expression during the mid-secretory phase was increased in the EMS group of patients.

Application of extended immunotherapy in advanced clear cell renal cell carcinoma treated with first-line combination of immune-checkpoint inhibitor and tyrosine kinase inhibitor.

Purpose: To evaluate the efficacy and safety of extended immunotherapy in first-line immune checkpoint inhibitors (ICIs)-tyrosine kinase inhibitors (TKIs) combination treatment for advanced renal cell carcinoma (RCC).

Patients And Methods: We retrospectively analyzed data from patients with advanced RCC who received first-line ICIs-TKIs combination treatment at West China Hospital of Sichuan University between October 2018 and July 2024. Patients who are assessed as having a disease control status after 2 years of continuous treatment will continue to receive immune checkpoint inhibitors until the inhibitors are discontinued due to disease progression or death.

An aptamer sensor based on AgNPs@MOF for surface-enhanced Raman spectroscopy detection of sulfadimethoxine in food.

A highly sensitive aptamer sensor (aptasensor) is proposed based on metal-organic frameworks-silver nanoparticles (AgNPs@MOF) to detect sulfadimethoxine (SDM) by surface-enhanced Raman spectroscopy (SERS). AgNPs@MOF with SERS activity was successfully fabricated by synthesizing AgNPs in situ on the surface of MIL-101(Fe), and SDM aptamer and Raman reporter 4-aminophenthiophenol (4-ATP) were selected as specific recognition elements and signal probes, respectively. When SDM was absent, the SDM aptamers were effectively adsorbed on the surface of AgNPs@MOF, thus keeping AgNPs@MOF in a dispersed state, resulting in a weakened SERS signal of 4-ATP.

Chlorpyrifos Influences Tadpole Development by Disrupting Thyroid Hormone Signaling Pathways.

Chlorpyrifos (CPF) is a widely used organophosphate insecticide with serious toxicological effects on aquatic animals. Although extensively studied for neurotoxicity and endocrine disruption, its stage-specific effects on amphibian metamorphosis and receptor-level interactions remain unclear. This study investigated the effects of CPF on metamorphosis at environmentally relevant concentrations (1.

Ursolic Acid as a Protective Agent Against UVB-Induced Metabolic and Epigenetic Alterations in Human Skin Keratinocytes: An Omics-Based Study.

This study aimed to assess how ursolic acid (UA) can protect human skin keratinocytes from damage caused by ultraviolet B (UVB) radiation. Utilizing an omics-based approach, we characterized the features of photodamage and investigated the potential of UA to reverse HaCaT cell subpopulation injury caused by UVB radiation. The most significant changes in metabolite levels after UA treatment were in pathways associated with phosphatidylcholine biosynthesis, arginine and proline metabolism.

Hydroxyl Spillover in Fe-Se Dual-Site Catalysts for Mixed Plastics Assay.

The complex composition of real plastic wastes poses a significant challenge for their large-scale disposal. A responsive on-site compositional analysis of plastics is informative in choosing downstream processing methods. Nanocatalyst-based assay kit is highly qualified for this scene; however, there remain no efficient nanocatalysts for plastics due to their highly inert chemistry.

Diverse Transient Chiral Dynamics in Evolutionary Distinct Photosynthetic Reaction Centers.

The evolution of photosynthetic reaction centers (RCs) from anoxygenic bacteria to higher-order oxygenic cynobacteria and plants highlights a remarkable journey of structural and functional diversification as an adaptation to environmental conditions. The role of chirality in these centers is important, influencing the arrangement and function of key molecules involved in photosynthesis. Investigating the role of chirality may provide a deeper understanding of photosynthesis and the evolutionary history of life on Earth.

A dual-functional monomer-based molecularly imprinted fluorescent aptasensor employing near-infrared carbon dots for selective detection of quinine in food.

A molecularly imprinted fluorescent aptasensor was designed for selective detection of quinine (Qn) based on dual functional monomers. In the sol-gel polymerization of molecularly imprinted polymers (MIPs), 3-aminopropyltriethoxysilane (APTES) and quinine aptamer (Apt) were employed as dual functional monomers, and Qn was the template molecule. Near-infrared carbon dots (RCDs) were used as fluorescence signal probe, and effectively avoided the interference of fluorescence emitted by Qn.

Microglial Nrf2-mediated lipid and iron metabolism reprogramming promotes remyelination during white matter ischemia.

Background: Oxidative stress and microglial activation are critical pathomechanisms in ischemic white matter injury. Microglia, as resident immune cells in the brain, are the main cells undergoing oxidative stress response. However, the role and molecular mechanism of oxidative stress in microglia have not been clearly elucidated during white matter ischemia.

In silico analysis of trehalose biosynthesis genes provides clues to reveal its roles in Avicennia marina adaptation to tidal submergence.

Trehalose has an important function for alleviating various abiotic stress in plants. Nevertheless, the functional and evolutionary characteristics of trehalose biosynthesis genes in mangrove plants is not documented. Here, using typical mangrove Avicennia marina, we found the trehalose content decreased in the roots and leaves and T6P increased significantly in the leaves under tidal submergence.

Operando bonding nickel thiolate with CdS as efficient photocatalyst for hydrogen evolution.

Employing metallic nanoclusters as cocatalysts for semiconductor-based photocatalysts and understanding their roles in enhancing photocatalytic performance is crucial. Herein, a nickel thiolate with cyclohexanethiol as the ligands (i.e.

Protein adsorption to poly(ethylenimine)-modified Sepharose FF: IX. Further studies on counterion effects and behavior in therapeutic protein separation.

Our previous studies on protein adsorption onto anion-exchangers of poly(ethylenimine) (PEI)-grafted Sepharose FF (PEI-Sepharose) proved their significantly improved performance over the commercial nongrafting anion-exchangers such as Q Sepharose FF, and it was found the protein adsorption behavior on PEI-Sepharose was more sensitive to counterions (Cl, SCN, HPO and SO). However, the complicated role of counterions has not been well interpreted due to their distinct chemical and physical characteristics. Thus, we have further studied the counterion effects by adding two halide ions (F and Br) to explore the effects of the three halide ions on bovine serum albumin adsorption and the results were compared with previous data.

CRISPR/Cas12a-Powered Electrochemical Platform for Dual-miRNA Detection via an AND Logic Circuit.

The CRISPR/Cas technology shows great potential in molecular detection and diagnostics. However, it is still challenging to detect multiple targets simultaneously using the CRISPR-Cas system. Herein, we ingeniously leverage the synergistic effect of two short single-stranded DNA activators to construct a CRISPR/Cas12a-driven electrochemical sensing platform based on an AND logic circuit ("AND" LC-CRISPR) for the simultaneous detection of dual miRNAs.

Fast Kinetics Enabled by Ion Enrichment Layer for Dendrite-Free Zinc Anode.

Aqueous zinc-ion batteries (AZIBs) are considered a promising choice for energy storage devices owing to the excellent safety and favorable capacity of the Zn anode. However, the uncontrolled dendrite growth of Zn anode severely constrains the practical applications of AZIBs. Herein, a novel ion enrichment layer of CuS is designed and constructed on the Zn foil surface to achieve dendrite-free Zn anode.

DNA-Assisted Separation of Nanoparticles.

DNA, as natural polymers, not only serves as the genetic material but also shows great potential as assistants for efficient and targeted nanoparticle separation. This is due to its high programmability, excellent biocompatibility, and stability for effectively binding with various types of nanoparticles or bioparticles, while modifying physical properties of target particles such as charge, mass and size. Researchers have achieved remarkable efficiency in separating nanoparticles by precisely designing DNA sequences and their interactions with target particles.

Versatile LaCoNiO Nanofiber Membrane for High Performance Oxygen Electrocatalysis over a Wide Temperature Range.

The oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) are key processes in numerous oxygen-involved applications over a wide temperature range. Despite advances in nanofiber engineering to increase active site density and catalytic efficiency for ORR/OER, conventional electrode fabrication methods often compromise the integrity of nanofibrous structures. Herein, a robust strategy is presented for the fabrication of LaCoNiO (LCN) nanofibrous membranes using optimized electrospinning techniques.

Ferroelectric Polarization Coupling Effect in BiFeO/α-InSe Ferroelectric Field Effect Transistor for Stable Non-volatile Memory.

Ferroelectric field-effect transistors (FeFETs) commonly utilize traditional oxide ferroelectric materials for their strong remanent polarization. Yet, integrating them with the standard complementary metal oxide semiconductor (CMOS) process is challenging due to the need for lattice matching and the high-temperature rapid thermal annealing process, which are not always compatible with CMOS fabrication. However, the advent of the ferroelectric semiconductor α-InSe offers a compelling solution to these challenges.

Clarifying the Active Structure and Reaction Mechanism of Atomically Dispersed Metal and Nonmetal Sites with Enhanced Activity for Oxygen Reduction Reaction.

Atomically dispersed transition metal (ADTM) catalysts are widely implemented in energy conversion reactions, while the similar properties of TMs make it difficult to continuously improve the activity of ADTMs via tuning the composition of metals. Introducing nonmetal sites into ADTMs may help to effectively modulate the electronic structure of metals and significantly improve the activity. However, it is difficult to achieve the co-existence of ADTMs with nonmetal atoms and clarify their synergistic effect on the catalytic mechanism.

Boosting Enzyme-Like Activity Through Controlled Coordination of Metal Single-Atoms in Rhombic Cavity of Graphyne.

The coordination number of metal single-atoms, being an important factor, should be precisely controlled as it affects both activity and specificity. Herein, the introduction of nitrogen into the para position of benzene in graphyne (2N-GY) is proposed as a carrier for anchoring metal single-atoms onto pyrazine nitrogen. These atoms are then coordinated to the center of the rhombic cavity, ensuring precisely controlled coordination numbers.

Differentiating reactive chlorine species for micropollutant abatement in chloride containing water by electrochemical oxidation process.

Electrochemical oxidation process (EOP) is promising for micropollutant degradation in water treatment, where chloride ions (Cl) are inevitable in aqueous systems, leading to the EOP/Cl system. The oxidation of Cl at anodes generates reactive chlorine species (RCS), including heterogeneous chlorine species (Cl), homogeneous free available chlorine (FAC), chlorine dioxide (ClO), and chlorine radicals (CRs). This study developed a method to differentiate various RCS responsible for the removal of carbamazepine in EOP/Cl using the RuO/IrO-Ti anode.

An improved ultrasound-assisted synthesis of phenytoin suitable for undergraduate education.

A convenient and efficient method for undergraduate student experiments involves the one-pot synthesis of phenytoin from benzoin. This method utilized ultrasonic irradiation in the presence of basic catalyst at room temperature and atmospheric pressure, resulting in shortened reaction times, good yields, and excellent reproducibility. By employing a low-cost ultrasonic cleaner for ultrasonic irradiation in student experiments, the need for expensive equipment investment during the teaching process can be minimized.

Increase or decrease? Impact of straw return on farmers' agricultural income in Jianghan Plain, Central China.

In the context of emphasizing on the food production, clean air guarding and sustainable development of agriculture, straw returning practices have been widely promoted, however, most studies have not linked straw returning adoption and smallholder's agricultural income in double-season rice cropping system. Based on a face-to-face survey data from 797 farmers in Jianghan Plain, Central China, we filled this gap by using the endogenous switching regression model to estimate the impact of farmer's straw returning on their agricultural income. The evidences show number of family laborers and technical training are the main factors influencing farmers' adoption of SR.

Prospects for potential therapy targeting immune‑associated factors in endometriosis (Review).

Endometriosis (EM) is a chronic inflammatory disease that is one of the most common causes of gynecological systemic lesions in women before menopause. The most representative histological feature of EM is that the endometrium appears outside of the uterine cavity, often in the ovary. Although it is generally accepted that the epithelial and stromal cells of the ectopic endometrium are not malignant, they still have numerous similarities to malignant tumors, including considerable changes to the immune microenvironment (immune monitoring disorder), the creation of a specific hormone environment, high levels of oxidative stress, chronic inflammation and abnormal immune cell regulation.