Serologic diagnosis of Entamoeba histolytica infection based on the gradient-based digital immunoassay.

Background: Entamoeba histolytica is a parasite that could cause severe amebiasis, an extremely contagious parasitic disease with critical clinical symptoms. Timely diagnosis and treatment of E. histolytica are crucial for preventing complications and fatalities.

BiS/BiO(OH) nanorods with internal electric field throughout the entire bulk phase as photoelectrochemical sensing platforms for CYFRA21-1 immunoassay.

Photoelectrochemical (PEC) immunosensors are highly promising tools for monitoring biochemical molecules. Constructing high-performance heterojunctions is a general method to improve the sensitivity of PEC immunosensors. The internal electric field (IEF) formed at the heterojunction interface plays a crucial role in coordinating the separation of photogenerated carriers.

A wearable platform for biochemical sweat analysis using photonic crystal hydrogel.

Wearable systems for health monitoring are highly desired in personal diagnostics and precision medicine while challenges remain in constructing such wearable systems with reliability and high performance. Herein, we report a wearable platform for non-invasive monitoring biomarkers in sweat. The device is composed of a butterfly-shaped like microfluidic platform in which responsive photonic crystal hydrogels are embedded in each butterfly wing as sensors.

A multifunctional biosensor for selective identification, sensitive detection and efficient photothermal sterilization of Salmonella typhimurium and Staphylococcus aureus.

Background: The foodborne pathogens, e.g., Salmonella typhimurium (S.

A dual-mode colorimetric/photothermal lateral flow biosensor based on Au/TiCT for HIV-DNA detection.

Background: Traditional lateral flow biosensors (LFBs), which utilize colorimetric signals as output, possess the virtues of simplicity and rapidity. However, it also suffers from insufficient sensitivity and limited reliability. It is well known that the results of LFBs can be false positive, and it is difficult to perform accurate quantification under low-abundance targets.

Cell membrane biomimetic magnetic fluorescent bifunctional nanoplatform for drug lead discovery.

Backgroud: Biomimetic nanoplatforms based on membrane coating strategies have received increasing attention in the field of medical research. However, it cannot perform biomedical imaging screening, which is essential for real-time identification. As a rich source of new drug discovery, traditional Chinese medicine (TCM) has made important contributions to the treatment of many diseases.

Biotinylation-based lateral flow assays for pathogenic and total bacteria detection.

Background: It is highly desirable to concurrently evaluate both pathogenic and total bacteria in water and food environments. As a point-of-care platform for biochemical tests, lateral flow assay (LFA) has been widely used for pathogenic bacteria due to its portability and fast time of outcome. However, traditional LFA was unable to detect total bacteria due to the lack of a universal antibody that could bind all the bacteria.

Tailoring Chirality and Optimizing Enantioselective Recognition in Strategic Defect Engineering of Chiral Metal-Organic Frameworks.

Introducing chiral molecules into metal-organic frameworks (MOFs) to obtain chiral MOFs (CMOFs), the tunability of their structures makes them a highly anticipated class of chiral materials for electrochemical sensing. However, the structure of CMOFs is often limited by synthesis challenges, and introducing chiral molecules into MOFs often leads to a decrease in their internal space. This study introduces a defect engineering strategy into the synthesis of CMOFs to obtain CMOFs with defects, which is an efficient synthesis method.

Biomimetic calcium-chelation nanoparticles reprogram tumor metabolism to enhance antitumor immunity.

Metabolic reprogramming within the tumor microenvironment poses a significant obstacle to the therapeutic efficacy of antitumor immunity. Here, inspired by the diverse programme of cholesterol metabolism between tumor and immune cells, a biocompatible carboxy-modified cyclodextrin carrier equipped with a biomimetic surface was developed to encapsulate FX11 and Avasimibe (RM-CDC@FX11&Ava) for synergistic antitumor metabolic therapy and immunotherapy. Through the manipulation of calcium levels using poly-carboxylic compounds to initiate cholesterol biosynthesis, RM-CDC@FX11&Ava dynamically regulates glycolysis and blocks cholesterol esterification to navigate metabolic reprogramming.

A novel small molecule NJH-13 induces pyroptosis via the Ca driven AKT-FOXO1-GSDME signaling pathway in NSCLC by targeting TRPV5.

Introduction: Pyroptosis represents a mode of programmed necrotic cell death (PCD), mediated by members of gasdermin family (GSDMs), such as GSDME. It is emerging as a promising approach for combating cancer. Notably, GSDME is the key modulator for the switch between apoptosis and pyroptosis in cells.

Cotargeting of thioredoxin 1 and glutamate-cysteine ligase in both imatinib-sensitive and imatinib-resistant CML cells.

Chronic myeloid leukemia (CML) is a type of malignancy characterized by harboring the oncogene Bcr-Abl, which encodes the constitutively activated tyrosine kinase BCR-ABL. Although tyrosine kinase inhibitors targeting BCR-ABL have revolutionized CML therapy, native and acquired drug resistance commonly remains a great challenge. Thioredoxin 1 (Trx1) and glutamate-cysteine ligase (GCL), which are two major antioxidants that maintain cellular redox homeostasis, are potential targets for cancer therapy and overcoming drug resistance.

Mast cell activation induced by tamoxifen citrate via MRGPRX2 plays a potential adverse role in breast cancer treatment.

Breast cancer is the most common malignant tumor endangering women's life and health. Tamoxifen citrate (TAM) is the first-line drug of adjuvant endocrine therapy for estrogen receptor-positive (ER) breast cancer patients. Some sporadic cases have described rare adverse reactions of TAM with potentially life-threatening dermatological manifestations, which were associated with skin allergy.

Schisandra chinensis lignans exerts endocannabinoids-like antidepressive effect: the phagocytotic relationship of activated CB2R-mediated M2 microglia and "stressed-but-viable" neuron.

Ethnopharmacological Relevance: Schisandra chinensis, a traditional functional Chinese medicine, is known for its ability to tonify the kidneys, calm the heart, and tranquilize the mind. Recent pharmacological research has demonstrated its anti-inflammatory and neuroprotective effects.

Aim Of The Study: We had previously demonstrated that Schisandra chinensis lignans (SCL) promote microglia polarization to M2 phenotype via targeting cannabinoid receptor type-2 (CB2R) to exert antidepressant effects.

Enhancing biobutanol production by optimizing acetone-butanol-ethanol fermentation from sorghum grains through strategic immobilization of amylolytic enzymes.

Tannin-containing sorghum grains, suitable for acetone-butanol-ethanol (ABE) production by Clostridium acetobutylicum, have required pretreatment to eliminate tannins inhibiting the strain's amylolytic activity. This study investigates biobutanol production enhancement by immobilizing enzymes on polydopamine-functionalized polyethersulfone (PES) membranes with magnetic nanoparticles for Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) processes. After multi-stage hot water treatment, TG3 sorghum (from the third stage) was used, where the enzyme-immobilized PES membrane produced 4.

Fabrication of alginate dialdehyde-gelatin crosslinked hydrogels incorporated with cinnamaldehyde nanoparticles for meat preservation.

Meat spoilage caused by Salmonella Typhimurium (S. typhimurium) contamination has become a major concern worldwide. Herein, the antibacterial mechanisms of cinnamaldehyde (CIN) against S.

UV responded polyvinyl alcohol bio-active films containing oregano essential oil microcapsules by chitosan-incorporated TiO: Physical properties, release characterizations, bio-functional performances and applications.

In the present study, with oregano essential oil (OEO) as the active ingredient and polyvinyl alcohol/citric acid (PVA/CA) as the composite matrix, ultraviolet (UV) responded PVA bio-active films incorporated with microcapsules, which were established by chitosan-incorporated titanium dioxide (TiO), were constructed. The UV light-triggered process changed the structure of films, including the degradation of PVA, the fracture of ester bonds and the breaking of glycosidic bonds. UV irradiation reduced the elongation at break, increased the light resistance ability, the surface hydrophobicity and the roughness, and accelerated the release of OEO in films.

Injectable biomimetic hydrogel based on modified chitosan and silk fibroin with decellularized cartilage extracellular matrix for cartilage repair and regeneration.

Cartilage defect repair remains a challenge for clinicians due to the limited self-healing capabilities of cartilage. Microenvironment-specific biomimetic hydrogels have shown great potential in cartilage regeneration because of their excellent biological properties. In this study, a hydrogel system consisting of p-hydroxybenzene propanoic acid-modified chitosan (PC), silk fibroin (SF) and decellularized cartilage extracellular matrix (DCM) was prepared.

Visible light-driven triazine-based S-scheme COF-TpTt@BiOBr heterojunction with oxygen vacancy for enhanced photocatalytic pollutants removal and hydrogen production.

S-scheme heterojunction is an effective tactic to improve photocatalytic property. But few studies on constructing heterojunction with BiOBr and covalent organic frameworks (COFs) are available. Herein, a novel series of COF-TpTt@BiOBr S-scheme heterojunctions with oxygen vacancies (OVs) were constructed via solvothermal method.

Effect of polypropylene microplastics on the performance of membrane bioreactors in wastewater treatment.

Membrane bioreactors (MBRs) can effectively remove microplastics (MPs) because of their good rejection performance. However, the influence of MP concentration and particle size on the short-term and long-term operation efficiency of MBRs remains unclear. To address this issue, this study investigated the effects of short-term stress and long-term accumulation of polypropylene microplastics (PP-MPs) with different particle sizes on the operational efficiency of MBRs by running three MBR systems at four concentration stages.

Community assembly and succession of the functional membrane biofilm in the anammox dynamic membrane bioreactor: Deterministic assembly of anammox bacteria.

The anammox dynamic membrane bioreactor (DMBR) exhibits potential for efficient nitrogen removal via anammox processes. The functional membrane biofilm in the anammox DMBR significantly enhances nitrogen removal, ensuring robust operation. Nevertheless, ecological mechanisms underpinning the nitrogen removal function of the membrane biofilm remain unclear.

Sacubitril/valsartan attenuates inflammation and myocardial fibrosis in Takotsubo-like cardiomyopathy.

Background: Takotsubo syndrome (TTS) primarily manifests as a cardiomyopathy induced by physical or emotional stress, remains a poorly understood condition with no established treatments. In this study, we investigated the potential of sacubitril/valsartan (sac/val) to increase the survival of TTS patients and reduce inflammation and myocardial fibrosis in experimental models.

Aim: This study aimed to evaluate whether sac/val could improve survival rates in TTS patients, mitigate cardiac remodeling in vivo, and explore its anti-inflammatory and antifibrotic mechanisms in vitro.

A corrole-based derivative as an ultra-fast response and high selective fluorescent probe for Mg and its application.

A new corrole derivative bearing 8-hydroxyquinoline moiety, which was labeled as 8-HQ-Corrole, serves as an efficient fluorescent probe for Mg in the presence of various interferents. This probe 8-HQ-Corrole displayed an ultra-fast response (2 s) to Mg with an impressive detection limit (33 nM) and a significant fluorescence enhancement, accompanying with a blue shift from 667 to 653 nm in fluorescence spectrum. MS and Job's plot analysis indicated that the enhanced fluorescence response was attributable to the formation of 1:2 stoichiometric complex between to Mg and 8-HQ-Corrole involving quinoline nitrogen and oxygen atoms.

Engineering a swich-on fluorescent probe for the visual detection of HOCl in envirnomental and living system.

Normal levels of HOCl can resist pathogen invasion and maintain cellular redox balance. However, excessive HOCl can easily harm the ecological environment and human health. Establishing a reliable approach for detection of HOCl can help resolve controversial issues regarding HOCl in physiological systems and help detect HOCl in complex environmental water samples.

Unveiling the effect of W and Co on PbO resistance over FeCe catalyst for low-temperature NH-SCR of NO.

The presence of PbO in the flue gas can poison FeCe catalyst, reducing its denitrification efficiency. In this work, the mechanism of PbO poisoning of FeCe catalyst, along with the effects of W and Co co-doping on the NO oxidation performance and PbO resistance of FeCe catalyst were investigated. The S of FeCe catalyst decreased and the crystallinity increased obviously after PbO poisoning.