Exposure of Caralluma tuberculata to biogenic selenium nanoparticles as in vitro rooting agent: Stimulates morpho-physiological and antioxidant defense system.

The commercial-scale production of Caralluma tuberculata faces significant challenges due to lower seed viability and sluggish rate of root growth in natural conditions. To overcome these obstacles, using phyto-mediated selenium nanomaterials as an in vitro rooting agent in plant in vitro cultures is a promising approach to facilitate rapid propagation and enhance the production of valuable therapeutic compounds. This study aimed to investigate the impact of phytosynthesized selenium nanoparticles (SeNPs) on the morphological growth attributes, physiological status, and secondary metabolite fabrication in in vitro propagated Caralluma tuberculata.

RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoring.

Background: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been conducted for improving its activity in photosynthesis through approaches like protein engineering. Concurrently, multiple biochemical and radiolabeling assays have been developed for determining its activity.

Health-promoting compounds in Lour and : Fruit essential oil exhibiting great potential for human health.

Unlabelled: The Zingiberaceae family serves as a diverse repository of bioactive phytochemicals, comprising approximately 52 genera and 1300 species of aromatic perennial herbs distinguished by their distinct creeping horizontal or tuberous rhizomes. Lour. and Crevost & Lemaire.

Molecular docking of bioactive compounds extracted and purified from selected medicinal plant species against covid-19 proteins and in vitro evaluation.

Bioactive compounds are secondary metabolites of plants. They offer diverse pharmacological properties. Peganum harmala is reported to have pharmaceutical effects like insecticidal, antitumor, curing malaria, anti-spasmodic, vasorelaxant, antihistaminic effect.

Insight into carbohydrate metabolism, protein quantification and mineral regulation in wheat ( L.) by the action of green synthesized silver nanoparticles (AgNPs) against heat stress.

In the present investigation, the role of GS-AgNPs treatment in wheat plants was carried out in reducing heat stress with the aim of facilitating scientists on this topic. The effect of GS-AgNPs against heat stress has rarely been deliberated in wheat plants, and only a few studies have been established earlier in this scenario. This work illustrated the effect of GS-AgNPs on the regulation of carbohydrates metabolism, SOD, proteins, crude fibers, and minerals changes in wheat plants.

Unlocking the interaction of organophosphorus pesticide residues with ecosystem: Toxicity and bioremediation.

Organophosphorus adulteration in the environment creates terrestrial and aquatic pollution. It causes acute and subacute toxicity in plants, humans, insects, and animals. Due to the excessive use of organophosphorus pesticides, there is a need to develop environmentally friendly, economical, and bio-based strategies.

Hierarchical plant extracts in silver nanoparticles preparation: Minuscular survey to achieve enhanced bioactivities.

Extracts obtained from (Lamiaceae) and (Rosaceae) were selected to utilize in the reduction and stabilization of silver nanoparticles (AgNPs) for achieving remarkable bioactivities. In brief, the cytotoxic potential of the as synthesize AgNPs was high at higher concentrations. In DPPH assay, maximum antioxidant potential was shown by AgNPs synthesized from .

Historical and current distribution ranges of the Asiatic black bear (Ursus thibetanus).

The current distribution of Asiatic black bear (Ursus thibetanus) is available on the IUCN Red List of Threatened species website; however, nothing is known about the historical extent and occurrence of this species. Therefore, we aimed to understand the historical distribution of the Asiatic black bear, and map and estimate its total size, to compare it with that of species current distribution. In addition, we analyzed a network of protected areas in the past and current ranges of the species.

Alleviation of cadmium toxicity in pea (Pisum sativum L.) through Zn-Lys supplementation and its effects on growth and antioxidant defense.

Cadmium significantly impacts plant growth and productivity by disrupting physiological, biochemical, and oxidative defenses, leading to severe damage. The application of Zn-Lys improves plant growth while reducing the stress caused by heavy metals on plants. By focusing on cadmium stress and potential of Zn-Lys on pea, we conducted a pot-based study, organized under completely randomized block design CRD-factorial at the Botanical Garden of Government College University, Faisalabad.

An application of fuzzy bipolar weighted correlation coefficient in decision-making problem.

Diagnosing and finding the disease in medical sciences is a complex procedure. The basic steps involved in finding starts with signs, symptoms, and test. This study is based on the diagnosis of a skin disorder.

Occurrence of Virulence Genes among Methicillin-Resistant Isolated from Subclinical Bovine Mastitis.

The occurrence of -induced subclinical mastitis holds significant implications for public health. This specific microorganism possesses a wide array of pathogenic factors that enable it to adhere to, colonize, invade, and infect the host. The objective of the current study was to assess the prevalence of , determine antimicrobial resistance patterns, and identify virulence genes of methicillin-resistant (MRSA) strains responsible for subclinical mastitis in bovines.

Insights into accumulation of active ingredients and rhizosphere microorganisms between Salvia miltiorrhiza and S. castanea.

Salvia miltiorrhiza is an important traditional herbal medicine, and its extracts could be used for treating cardiovascular disease. Although these medicinal compounds are functionally similar, their wild relative, S. castanea, produces significantly different concentrations of these compounds.

Temperature variability during the growing season affects the quality attributes of table grapes in Pothwar-insight from a new emerging viticulture region in South Asia.

Rising air temperature due to climate change has posed a mammoth challenge to global viticulture and key berry quality traits are compromised. Exploring the effects of seasonal temperature variability on berry ripening and quality attributes in different viticulture regions may help in sustainable viticulture industry. The present research was designed to explore the effect of temperature variables on key quality attributes of table grape cultivars in Pothwar region of Pakistan.

Bioinformatics, RNA sequencing, and targeted bisulfite sequencing analyses identify the role of PROM2 as a diagnostic and prognostic biomarker.

Objectives: Prominin 2 (PROM2) gene has been reported as a molecular biomarker of human cancers; however, its role is still controversial. This study was therefore arranged to seek the role of PROM2 in different cancers with Bioinformatics and in vitro analyses.

Methods: A combination of bioinformatics and molecular experiments.

A pan-cancer analysis of pituitary tumor-transforming 3, pseudogene.

Background: Although evidence regarding pituitary tumor-transforming 3, pseudogene (PTTG3P) involvement in human cancers has been acquired via human and animal model-based molecular studies, there is a lack of pan-cancer analysis of this gene in human tumors.

Methods: Tumor-causing effects of PTTG3P in 24 human tumors were explored using The Cancer Genome Atlas (TCGA) datasets from different bioinformatics databases and applying in silico tools such as The University of ALabama at Birmingham CANcer (UALCAN), Human Protein Atlas (HPA), Kaplan Meier (KM) plotter, cBioPortal, Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), Cytoscape, Database for Annotation, Visualization, and Integrated Discovery (DAVID), Tumor IMmune Estimation Resource (TIMER), and Comparative Toxicogenomics Database (CTD). Then, via in vitro experiments, including RNA sequencing (RNA-seq) and targeted bisulfite sequencing (bisulfite-seq), expression and promoter methylation levels of PTTG3P were verified in cell lines.

Struvite separation from wastewater and its use with sulfur-oxidizing bacteria improves phosphorus utilization in alkaline soil.

A major portion of phosphatic fertilizer comes from the limiting natural resource, rock phosphate, which demands a timely alternative. Struvite, a crystalline mineral of low solubility, is a worthwhile alternative. Evaluation of the local wastewater streams for their ability to precipitate struvite and its capability as phosphatic fertilizer under an alkaline soil environment was studied.

Effects of inoculation of corn silage with on silage fermentation characteristics, nutrient digestibility, mycoflora and aflatoxin production.

In the present study, fresh whole corn plants along with half milk kernels line were treated with live and hydrolysed yeast with different concentrations before ensiling and kept in airtight bags and then into mini silos in order to achieve anaerobic conditions for proper fermentation. The buckets were opened after different time intervals to characterise the material, quick acidification, dry matter recovery, and aerobic stability of silage respectively. Moreover, mycoflora and aflatoxin contamination were also analysed.

Acute Toxicity, Anti-diabetic, and Anti-cancerous Potential of Trillium Govanianum-conjugated Silver Nanoparticles in Balb/c Mice.

Background: The current study aimed to develop an economic plant-based therapeutic agent to improve the treatment strategies for diseases at the nano-scale because Cancer and Diabetes mellitus are major concerns in developing countries. Therefore, and antidiabetic and anti-cancerous activities of conjugated silver nanoparticles were assessed.

Methods: In the current study synthesis of silver nanoparticles using and characterization were done using a scanning electron microscope, UV-visible spectrophotometer, and FTIR analysis.

Towards deep learning based smart farming for intelligent weeds management in crops.

Introduction: Deep learning (DL) is a core constituent for building an object detection system and provides a variety of algorithms to be used in a variety of applications. In agriculture, weed management is one of the major concerns, weed detection systems could be of great help to improve production. In this work, we have proposed a DL-based weed detection model that can efficiently be used for effective weed management in crops.

Toxicity assessment of microplastic (MPs); a threat to the ecosystem.

Plastic is now considered part and parcel of daily life due to its extensive usage. Microplastic (MP) pollution is becoming a growing worry and has been ranked as the second most critical scientific problem in the realm of ecology and the environment. Microplastics are smaller in size than the plastic and are more harmful to biotic and as well as abiotic environments.

Combination of enzymatically hydrolyzed potato powder with skimmed milk powder on the quality improvements of yogurt during refrigeration storage.

Unlabelled: This study aimed to prepare a stirred type of fat-free yogurt from enzymatically hydrolyzed potato powder (EHPP) and skimmed milk powder (SMP) without changing its quality and consumer acceptance. The yogurt formulations prepared contained different amount of EHPP 0, 10, 25 and 50% and were stored for 28 days at 4 °C and observed that with increasing substitution ratio, acid production was increased while the viability of lactic acid bacteria was decreased after 28 days of storage at 4 °C. The antioxidant activities (2-Diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power (FRAP) of the yogurt were increased with increasing EHPP over the storage period.

Sero-Epidemiology and Associated Risk Factors of Foot-and-Mouth Disease (FMD) in the Northern Border Regions of Pakistan.

The present cross-sectional survey was carried out to investigate the distribution and risk factors of FMD in Pakistan's northern border regions. About 385 serum samples were compiled from small ruminants (239) and large ruminants (146) and tested using 3ABC-Mab-bELISA. An overall apparent seroprevalence of 67.