Development and evaluation of isradipine via rutin-loaded coated solid-lipid nanoparticles.

The objective was to develop a stable and non-compliance coated solid-lipid nanoparticles (coated SLN) using polymer (Eudragit L100) and lipoid (glycerol monostearate: soya lecithin) for partial dose reduction of isradipine [ISR; 2.5 mg by combination of bioenhancing agent (rutin; Ru) in equivalent ratio]. The physicochemical characterizations were performed by FT-IR and DSC of elected model drug (ISR), drug mixer with Ru/polymer and coated SLN with Ru (ONbp); the resulted distinctive peaks demonstrated that no chemical interaction and incompatibility found between them.

Nano-colloidal carrier polymeric coating for oral delivery of isradipine.

Our research objective was to develop, characterize, and optimize stable form of nano-colloidal carrier with Eudragit-coated solid lipid nanobioparticles (SLNbp) for oral delivery of isradipine (ISR). To achieve, a three factors, i.e.

Formulation and Evaluation of Novel Solid Lipid Microparticles for the Sustained Release of Ofloxacin.

Objective: The aim of this study was to prepare solid lipid microparticles (SLM) with incorporated ofloxacin, suitable for oral delivery.

Methods: Ofloxacin-loaded SLM were prepared using stearic acid and chloroform as lipid matrix and Tween-80 as surfactant, by high shear homogenization technique and followed by lyophilization. The physiochemical characterization of SLMs were investigated by scanning electron microscopy, transmission electron microscopy, zeta potential, zone of inhibition, in-vitro study, ex-vivo study and stability study.

A Comprehensive Review on Role of Nanoparticles in Therapeutic Delivery of Medicine.

Background: The effective targeted drug delivery system is significant for future development of medicinal product and healthcare. There are also different types of nanostructures which include solid lipid nanoparticles, liposomes, dendrimers, nanostructured lipid carriers, lipid drug conjugate, liquid crystalline particles, polymeric nanocrystals, polymeric nanoparticles and superparamagnetic nanoparticles which have been considered as advanced carriers in drug delivery system. Method & Discussion: In this regard, nano carriers are bringing revolution in therapeutic delivery of drug as compared to conventional delivery systems by overcoming the limitations of usual methods.

Design, Optimization and Characterization of Granisetron HCl Loaded Nano-gel for Transdermal Delivery.

Background & Objective: Our research objective was to design, develop, optimize and characterize Granisetron HCl transdermal gel in order to minimize side effects associated with oral delivery.

Method: A statistical design was practically applied for further optimization and preparation of transfersomal gel using Box-Behnken methodology at three levels. The selected independent and dependent variables were Lipoid, surfactant and sonication time and encapsulation efficiency, size and flux correspondingly.

Systematic development of optimized SNEDDS of artemether with improved biopharmaceutical and antimalarial potential.

The current studies entail systematic development of self-nanoemulsifying drug delivery systems (SNEDDS) containing medium-chain triglycerides (MCTs) and long-chain triglycerides (LCTs) for augmenting the biopharmaceutical performance of artemether. Equilibrium solubility and pseudoternary phase diagram studies facilitated selection of Captex 355 and Ethyl oleate as MCTs and LCTs, and Cremophor RH 40 and Tween 80 as surfactants, while Transcutol HP as cosolvent for formulating the SNEDDS. Systematic optimization was performed employing the Box-Behnken design taking concentrations of lipid, surfactant and cosolvent as the critical material attributes (CMAs), while evaluating for globule size, emulsification time, dissolution efficiency and permeation as the critical quality attributes (CQAs).

Anti-inflammatory, Analgesic and Antiulcer properties of Porphyra vietnamensis.

Objectives: Aim of the present work was to investigate the anti-inflammatory, analgesic and antiulcer effects of red seaweed Porphyra vietnamensis (P. vietnamenis).

Materials And Methods: Aqueous (POR) and alcoholic (PE) fractions were successfully isolated from P.

Taguchi design for optimization and development of antibacterial drug-loaded PLGA nanoparticles.

This research report was to develop Cefixime loaded polylactide-co-glycolide (PLGA) nanoparticles using modified precipitation method. TEM analysis indicated formation of well-formed, smooth, spherical nanoparticles with no aggregates whereas XRD recommended dispersion of drug in PLGA carrier system in amorphous form. The polymer and stabilizer concentration and organic to aqueous ratio were found to be significant factors for nanoparticles and their optimization using Taguchi design (L9).

Nano-transfersomes as a novel carrier for transdermal delivery.

The aim of this study was to design and optimize a nano-transfersomes of Diclofenac diethylamine (DDEA) and Curcumin (CRM). A 3(3) factorial design (Box-Behnken) was used to derive a polynomial equation (second order) to construct 2-D (contour) and 3-D (Response Surface) plots for prediction of responses. The ratio of lipid to surfactant (X1), weight of lipid to surfactant (X2) and sonication time (X3) (independent variables) and dependent variables [entrapment efficiency of DDEA (Y1), entrapment efficiency of CRM (Y2), effect on particle size (Y3), flux of DDEA (Y4), and flux of CRM (Y5)] were studied.

Optimization and formulation design of carbopol loaded Piroxicam gel using novel penetration enhancers.

The aim of the study was to develop and optimize Piroxicam transdermal gel formulation using three-factor, three-level Box-Behnken design by deriving a second-order polynomial equation to construct contour plots for prediction of responses as three selected independent variables with ratio of carbopol 974 (X1), ratio of propylene glycol (PG) (X2) and ratio of ethanol (X3). The dependent variables studied were the skin permeation rate of piroxicam (Y1), viscosity of the gel (Y2) and pH of the gel (Y3). Response surface plots were drawn, statistical validity of the polynomials was established to find the compositions of optimized formulation which was evaluated using the vertical Franz-type diffusion cell.

Optimization and evaluation of gastroretentive ranitidine HCl microspheres by using design expert software.

The current study involves the development and optimization of their drug entrapment and ex vivo bioadhesion of multiunit chitosan based floating system containing Ranitidine HCl by ionotropic gelation method for gastroretentive delivery. Chitosan being cationic, non-toxic, biocompatible, biodegradable and bioadhesive is frequently used as a material for drug delivery systems and used to transport a drug to an acidic environment where it enhances the transport of polar drugs across epithelial surfaces. The effect of various process variables like drug polymer ratio, concentration of sodium tripolyphosphate and stirring speed on various physiochemical properties like drug entrapment efficiency, particle size and bioadhesion was optimized using central composite design and analyzed using response surface methodology.

In-vitro cytotoxic activity of β-Sitosterol triacontenate isolated from Capparis decidua (Forsk.) Edgew.

Objective: To study the isolation and characterization of the constituent responsible for the cytotoxic activity of the ethanolic extract of stem of Capparis decidua (C. decidua).

Methods: The preliminary cytotoxic effect of isolated compound (β-Sitosterol triacontenate) was investigated by MTT assay on A549 solid tumor cells.

In vitro anticancer activity of stachydrine isolated from Capparis decidua on prostate cancer cell lines.

In this article we report our work on the isolation, characterisation and evaluation of in vitro anticancer activity of stachydrine on solid tumour cells. The in vitro activity was assessed by MTT assay and propidium iodide (PI) staining. Further, an attempt was also made to check the effect of stachydrine on the invasion and metastasis of cancer cells by inhibiting the expression of chemokine receptors (CXCR3 and CXCR4).

Mechanism of action of flavonoids as anti-inflammatory agents: a review.

Flavonoids are polyphenolic compounds that occur ubiquitously in plants having a variety of biological effects both in vitro and in vivo. They have been found to have antimicrobial, antiviral, anti-ulcerogenic, cytotoxic, anti-neoplastic, mutagenic, antioxidant, antihepatotoxic, antihypertensive, hypolipidemic, antiplatelet and anti-inflammatory activities. Flavonoids also have biochemical effects, which inhibit a number of enzymes such as aldose reductase, xanthine oxidase, phosphodiesterase, Ca(+2)-ATPase, lipoxygenase, cycloxygenase, etc.