Progress of Pathogenesis in Pediatric Multifocal Atrial Tachycardia.

Multifocal atrial tachycardia (MAT) is defined as irregular P-P, R-R, and P-R intervals, isoelectric baseline between P waves, and ventricular rate over 100 beats/min. Although the prognosis of pediatric MAT in most patients is favorable, adverse outcomes of MAT have been reported, such as cardiogenic death (3%), respiratory failure (6%), or persistent arrhythmia (7%), due to delayed diagnosis and poorly controlled MAT. Previous studies demonstrated that pediatric MAT is associated with multiple enhanced automatic lesions located in the atrium or abnormal automaticity of a single lesion located in the pulmonary veins multiple pathways to trigger electrical activity.

Predicting prostate cancer specific-mortality with artificial intelligence-based Gleason grading.

Background: Gleason grading of prostate cancer is an important prognostic factor, but suffers from poor reproducibility, particularly among non-subspecialist pathologists. Although artificial intelligence (A.I.

Sbk2, a Newly Discovered Atrium-Enriched Regulator of Sarcomere Integrity.

Background: Heart development relies on tight spatiotemporal control of cardiac gene expression. Genes involved in this intricate process have been identified using animals and pluripotent stem cell-based models of cardio(myo)genesis. Recently, the repertoire of cardiomyocyte differentiation models has been expanded with iAM-1, a monoclonal line of conditionally immortalized neonatal rat atrial myocytes (NRAMs), which allows toggling between proliferative and differentiated (ie, excitable and contractile) phenotypes in a synchronized and homogenous manner.

Immune cells in cardiac repair and regeneration.

The immune system is fundamental to tissue homeostasis and is the first line of defense following infection, injury or disease. In the damaged heart, large numbers of immune cells are recruited to the site of injury. These cells play an integral part in both repair by scar formation and the initiation of tissue regeneration.

Hierarchical confounder discovery in the experiment-machine learning cycle.

The promise of machine learning (ML) to extract insights from high-dimensional datasets is tempered by confounding variables. It behooves scientists to determine if a model has extracted the desired information or instead fallen prey to bias. Due to features of natural phenomena and experimental design constraints, bioscience datasets are often organized in nested hierarchies that obfuscate the origins of confounding effects and render confounder amelioration methods ineffective.

Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.

Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines. Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi. The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.

Spatial transcriptomics of dorsal root ganglia identifies molecular signatures of human nociceptors.

Nociceptors are specialized sensory neurons that detect damaging or potentially damaging stimuli and are found in the dorsal root ganglia (DRG) and trigeminal ganglia. These neurons are critical for the generation of neuronal signals that ultimately create the perception of pain. Nociceptors are also primary targets for treating acute and chronic pain.

Copper acetate aerosols: A possible tool complementary to vaccination in fight against SARS-CoV-2 and variants replication.

In SARS-CoV-2, at the S1/S2 furin cleavage site, a four amino acid insert (P-R-R-A) not found in closely related corona viruses, has been shown to facilitate entry into respiratory epithelial cells and promote virus transmission, infectivity and virulence. By cupric aerosol treatment, complexation of these four amino acids (-P-R-R-A-), at the spike (S) protein site will lead to a conformational change possibly impeding SARS-CoV-2 replication process in the respiratory track. Since these four amino acids yield strong and stable copper complexes, subsequent to a steric hindrance, this complexation will disturb the furin-like protease cleavage at the spike protein site as it has been recently shown in vitro with copper gluconate.

Personalized subcutaneous implantable cardioverter-defibrillator sensing vectors generated by mathematical rotation increase device eligibility whilst preserving device performance.

Aims: Approximately 5.7% of potential subcutaneous implantable cardioverter-defibrillator (S-ICD) recipients are ineligible by virtue of their vector morphology, with higher rates of ineligibility observed in some at-risk groups. Mathematical vector rotation is a novel technique that can generate a personalized sensing vector, one with maximal R:T ratio, using electrocardiogram (ECG) signal recorded from the present S-ICD location.

Tunable Second-Level Room-Temperature Phosphorescence of Solid Supramolecules between Acrylamide-Phenylpyridium Copolymers and Cucurbit[7]uril.

A series of solid supramolecules based on acrylamide-phenylpyridium copolymers with various substituent groups (P-R: R=-CN, -CO Et, -Me, -CF ) and cucurbit[7]uril (CB[7]) are constructed to exhibit tunable second-level (from 0.9 s to 2.2 s) room-temperature phosphorescence (RTP) in the amorphous state.

Choroidal Changes in Eyes With Polypoidal Choroidal Vasculopathy After Anti-VEGF Therapy Imaged With Swept-Source OCT Angiography.

Purpose: Swept-source optical coherence tomography angiography was used to investigate choroidal changes and their association with pigment epithelial detachments (PEDs) in eyes with polypoidal choroidal vasculopathy (PCV) after treatment with vascular endothelial growth factor (VEGF) inhibitors.

Methods: Patients with treatment-naïve PCV were included and underwent anti-VEGF therapy. Mean choroidal thickness (MCT), choroidal vascularity index (CVI), and PED volume measurements were obtained before and after treatment.

An NHC-Stabilized H GeBH Precursor for the Preparation of Cationic Group 13/14/15 Hydride Chains.

The synthesis, characterization and reactivity studies of the NHC-stabilized complex IDipp ⋅ GeH BH OTf (1) (IDipp=1,3-bis(2,6-diisopropylphenyl)imidazolin-2-ylidene) are reported. Nucleophilic substitution of the triflate (OTf) group in 1 by phosphine or arsine donors provides access to the cationic group 13/14/15 chains [IDipp ⋅ GeH BH ERR R ] (2 E=P; R, R =H; R = Bu; 3 E=P; R=H; R , R =Ph; 4 a E=P; R, R , R =Ph; 4 b E=As; R, R , R =Ph). These novel cationic chains were characterized by X-ray crystallography, NMR spectroscopy and mass spectrometry.

Obesity Does Not Protect From Subarachnoid Hemorrhage: Pooled Analyses of 3 Large Prospective Nordic Cohorts.

Background: Several population-based cohort studies have related higher body mass index (BMI) to a decreased risk of subarachnoid hemorrhage (SAH). The main objective of our study was to investigate whether the previously reported inverse association can be explained by modifying effects of the most important risk factors of SAH-smoking and hypertension.

Methods: We conducted a collaborative study of three prospective population-based Nordic cohorts by combining comprehensive baseline data from 211 972 adult participants collected between 1972 and 2012, with follow-up until the end of 2018.

Marine fish assemblages of Eastern Brazil: An update after the world's largest mining disaster and suggestions of functional groups for biomonitoring long-lasting effects.

When the Fundão dam collapsed in Brazil, 50 million m of iron ore tailings were released into the Doce river, resulting in the world's largest mining disaster. The contaminated mud was transported 668 km downstream of the Doce river and reached the Atlantic Ocean 17 days after the collapse. Seven months later, there was evidence that the tailings had reached the largest and richest coral reef formation in the South Atlantic Ocean.

A neuronal basis of iconic memory in macaque primary visual cortex.

After a briefly presented visual stimulus disappears, observers retain a detailed representation of this stimulus for a short period of time. This sensory storage is called iconic memory. We measured iconic memory in the perception of monkeys and its neuronal correlates in the primary visual cortex (area V1).

Electronic and Vibrational Decoupling in Chemically Exfoliated Bilayer Thin Two-Dimensional VO.

The synthesis of high-quality two-dimensional (2D) transition metal oxides is challenging compared to 2D transition metal dichalcogenides as a result of the exotic surface changes that can appear during formation. Herein, we report the synthesis of bilayer 2D VO nanosheets with a thickness of ∼1 nm using the chemical exfoliation method and a comprehensive study on the vibrational and optical properties of bilayer 2D VO. We report, for the first time, a thickness-dependent blue shift of 1.

The ROR1 antibody-drug conjugate huXBR1-402-G5-PNU effectively targets ROR1+ leukemia.

Antibody-drug conjugates directed against tumor-specific targets have allowed targeted delivery of highly potent chemotherapy to malignant cells while sparing normal cells. Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is an oncofetal protein with limited expression on normal adult tissues and is overexpressed on the surface of malignant cells in mantle cell lymphoma, acute lymphocytic leukemia with t(1;19)(q23;p13) translocation, and chronic lymphocytic leukemia. This differential expression makes ROR1 an attractive target for antibody-drug conjugate therapy, especially in malignancies such as mantle cell lymphoma and acute lymphocytic leukemia, in which systemic chemotherapy remains the gold standard.

The MURAL collection of prostate cancer patient-derived xenografts enables discovery through preclinical models of uro-oncology.

Preclinical testing is a crucial step in evaluating cancer therapeutics. We aimed to establish a significant resource of patient-derived xenografts (PDXs) of prostate cancer for rapid and systematic evaluation of candidate therapies. The PDX collection comprises 59 tumors collected from 30 patients between 2012-2020, coinciding with availability of abiraterone and enzalutamide.

Current Perspective of COVID-19 on Neurology: A Mechanistic Insight.

SARS-CoV-2, the novel coronavirus and the causative organism of the Covid-19 pandemic wreaked havoc worldwide producing asymptomatic to symptomatic cases leading to significant morbidity and mortality even after infection. Most of the countries reported a mortality rate of 2-3 % majorly due to cardiorespiratory failures. Recent studies highlighted the neurological involvement playing a key role in cardiorespiratory failures and other symptoms such as headache, anosmia, and ageusia observed in Covid-19 patients.

Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision.

In highly dispersion compensated Fourier domain mode locked (FDML) lasers, an ultra-low noise operation can only be achieved by extremely precise and stable matching of the filter tuning period and light circulation time in the cavity. We present a robust and high precision closed-loop control algorithm and an actively cavity length controlled FDML laser. The cavity length control achieves a stability of ∼0.

RespiraConNosotros: A Viable Home-Based Telerehabilitation System for Respiratory Patients.

Currently, there are more than 1.55 million cases of SARS-CoV-2 infection in Spain. Of these, it is estimated that around 45% will present respiratory complications, which represents approximately 620,000 patients who will need respiratory rehabilitation.

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered .

Prevention of COVID-19 on a global scale will require the continued development of high-volume, low-cost platforms for the manufacturing of vaccines to supply on-going demand. Vaccine candidates based on recombinant protein subunits remain important because they can be manufactured at low costs in existing large-scale production facilities that use microbial hosts like ( ). Here, we report an improved and scalable manufacturing approach for the SARS-CoV-2 spike protein receptor binding domain (RBD); this protein is a key antigen for several reported vaccine candidates.

Interpretable survival prediction for colorectal cancer using deep learning.

Deriving interpretable prognostic features from deep-learning-based prognostic histopathology models remains a challenge. In this study, we developed a deep learning system (DLS) for predicting disease-specific survival for stage II and III colorectal cancer using 3652 cases (27,300 slides). When evaluated on two validation datasets containing 1239 cases (9340 slides) and 738 cases (7140 slides), respectively, the DLS achieved a 5-year disease-specific survival AUC of 0.

Evaluating heart electrical activities and cardiac arrhythmias of Holstein cows during ageing by short-term electrocardiography in comparison with 24-hour holter-monitoring.

Short-term electrocardiography is one of the most suitable tools to study the electrical activity of the heart, but the use of a tool such as a Holter-monitor with the ability to assess the long-term of the heart electrical activity, can provide more accurate information about these activities by comparing the results. It is possible to understand the superiority of each over the other and the resulting differences. Therefore, 60 female Holstein cows in 10 age groups, including 1 day, 1, 3, 6 months, 1, 2, 3, 4, 6 and 8 years were included in the study (6 heads in each age group).