Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root.

A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in Salvia miltiorrhiza Bge. root. In this study, a series of polarity-adjustable DESs were prepared using n-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined.

Erythromycin Repurposing for Hepatocellular Carcinoma Treatment: Targeting CD24 to Enhance Anti-Tumor Immunity.

Hepatocellular carcinoma (HCC) is a malignant tumor, making up about 90% of all primary liver cancers and ranking as a top cause of cancer mortality globally. With the severe side effects of conventional radiotherapy and chemotherapy, there's an ongoing quest for less toxic treatment options. CD24, a highly glycosylated membrane surface protein, plays a role in cancer cell adhesion and metastasis.

Novel CoFe-supported UiO-66-derived ZrO for rapid activation of peracetic acid for sulfamethoxazole degradation.

The leaching of toxic metals is still problematic for heterogeneous metal catalysts in activating peracetic acid (PAA). Herein, CoFe/U-ZrO was synthesized by loading CoFe onto the metal-organic framework (UiO-66) derived ZrO (U-ZrO) for PAA activation. The high porosity and specific surface area of UiO-66 enable efficient embedding and uniform dispersion of CoFe particles into pore channels.

Interferon-γ-stimulated antigen-presenting cancer-associated fibroblasts hinder neoadjuvant chemoimmunotherapy efficacy in lung cancer.

Conventional neoadjuvant chemotherapy provides limited benefit for patients with resectable non-small cell lung cancer (NSCLC). Recently, neoadjuvant chemoimmunotherapy (NCIT) has transformed the perioperative management of NSCLC by priming systemic anti-tumor immunity before surgery, yet it remains ineffective for at least 50% of patients. Through single-cell sequencing analysis of our NCIT cohort, we identify that antigen-presenting cancer-associated fibroblasts (apCAFs) can impede the efficacy of NCIT.

Primary ciliary protein kinase A activity in the prefrontal cortex modulates stress in mice.

Primary cilia are cellular antennae emanating from vertebrate cell surfaces to sense and transduce extracellular signals intracellularly to regulate cell behavior and function. However, their signal sensing and physiological functions in neocortical neurons remain largely unclear. Here, we show that, in response to various animal stressors, primary cilia in the mouse prefrontal cortex (PFC) exhibit consistent axonemal elongation.

Effective compound combination of Bufei Yishen formula ameliorates PM2.5-induced COPD by inhibiting mitochondrial oxidative stress through SIRT3-mediated FOXO3 deacetylation.

Background: Fine particulate matter (PM2.5) exposure significantly contributes to the development of chronic obstructive pulmonary disease (COPD). However, the underlying mechanisms remain inadequately elucidated, and there is a lack of effective clinical treatments.

Enhancing the Stability of Sweet Protein Neoculin for Food Applications Using Computational Strategies.

Sweet proteins, known for their high sweetness and low caloric content, offer great potential as safe, low-calorie sweeteners without the negative health effects associated with sugar or artificial alternatives. Neoculin, unique for its dual properties of sweetness and taste-modifying, holds significant promise for food industry applications. However, its low thermal stability limits its broader use in food processing, highlighting the need to improve its stability.

Advances in the Regulation of Hematopoietic Homeostasis by Programmed Cell Death Under Radiation Conditions.

The application of nuclear energy and the frequent occurrence of nuclear contamination have made radiation safety a major challenge to global public health. As a radiation-sensitive target organ, bone marrow is susceptible to both acute and chronic damage effects of ionizing radiation on the hematopoietic system. Researchers have demonstrated that radiation disrupts hematopoietic homeostasis through direct damage to hematopoietic stem cells, which inhibits hematopoietic regeneration indirectly through damage to hematopoietic progenitor cells and their downstream cell populations.

Modifying the Dzyaloshinskii-Moriya Interaction via Disruption of Ordered Intercalation in a van der Waals Magnet.

The intercalation of magnetic atoms into van der Waals (vdW) gaps offers a unique opportunity to manipulate the Dzyaloshinskii-Moriya interaction (DMI) and magnetic structures by modifying the local atomic structures in vdW magnets. Herein, a new strategy is proposed for modifying DMI by disrupting the atomic-scale order within the Cr sublattice of Cr-intercalated 2H-TaS, i.e.

Accelerated Hydrogel Strengthening: Synergy between Mechanical Training and Lignin Intake.

The construction of high-strength hydrogels is essential for engineering applications but is often limited by poor durability under stress. Current post-treatment methods are inefficient and time consuming. Inspired by muscle building, we propose a green, efficient, and synergistic enhancement method.

Fluorinated Cyclodextrin Supramolecular Nanoassembly Enables Oxygen-Enriched and Targeted Photodynamic Therapy.

Photodynamic therapy has become a promising treatment modality against many diseases, but its dilemma-the intrinsic hypoxia of solid tumors and the high oxygen dependence for generation of cytotoxic species-has seriously hampered its practical translation. Herein a binary supramolecular nanocarrier, which is composed of fluorocarbon chain-appended β-cyclodextrin as an oxygen carrier and adamantane-grafted hyaluronic acid as a cell-targeting agent, can deliver different types of photosensitizers by multiple noncovalent interactions. Superior to the alkylated counterpart, the fluorinated amphiphilic β-cyclodextrin can spontaneously form a nanoparticulate assembly and exhibit high oxygen-enrichment performance.

The Relationship Between Serum SFRP5, ApoA-I, HDL3-C Level and In-Stent Restenosis After PCI in Acute Myocardial Infarction and the Combined Predictive Value.

This study aims to investigate the relationship between serum secreted frizzled-related protein 5 (SFRP5), apolipoprotein A-I (ApoA-I), high-density lipoprotein 3-cholesterol (HDL3-C) and in-stent restenosis (ISR) after percutaneous coronary intervention (PCI) in acute myocardial infarction (AMI) and their combined predictive value. The clinical data of 128 AMI patients who underwent PCI in our hospital from July 2020 to July 2023 were retrospectively analyzed. After 12 months of follow-up, the patients were divided into the ISR group (24 cases) and the non-ISR group (104 cases) according to the results of coronary angiography.

Plac1 Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer.

Cancer/testis antigen (CTA) family is restricted to germline and tumor cells and plays an important role during cancer initiation and progression. Five single-cell and two bulk RNA-seq datasets are integrated to screen genes in the CTAs family, revealing that Placenta specific protein 1 (Plac1) is specifically expressed in head and neck squamous cell carcinoma (HNSCC) cells. Sp1 Transcription (SP1) is identified as a specific regulator of Plac1, which is confirmed by cleavage under targets and tagmentation (CUT&Tag)-seq.

Optimization of Lipid Nanoparticles with Robust Efficiency for the Delivery of Protein Therapeutics to Augment Cancer Immunotherapy.

Lipid nanoparticles (LNPs) have been successful in delivering nucleic acids like siRNA and mRNA, but face challenges in protein delivery due to limited protein encapsulation and endosome escape. In this study, a family of LNPs is developed with robust high efficiency in addressing the multiple barriers in cytosolic protein delivery by incorporating clinically approved ionizable lipids into traditional cationic LNPs. The combination of cationic and ionizable lipids enables efficient protein binding and endosomal escape.

Dopant-Regulated Piezocatalysts Evoke Sonopiezoelectric and Enzymatic PANoptosis for Synergistic Cancer Therapy.

Piezocatalyst-enabled sonopiezoelectric therapy offers noninvasive treatment with high spatiotemporal selectivity, yet existing piezocatalysts are limited by suboptimal efficacy, cancer cell resistance to oxidative stress, and biosafety concerns. Here, hafnia (HfO), one of the only few FDA-approved inorganic nanomaterials for clinical trials, is identified as a promising piezocatalyst with high translational potential for sonopiezoelectric and enzymatic PANoptosis-boosted nanocatalytic therapy. Specifically, engineered transition metal-substituted HfO nanocatalysts are synthesized to optimize piezoelectric and enzyme-mimicking activities.

Osteosarcoma Cell-Derived Migrasomes Promote Macrophage M2 Polarization to Aggravate Osteosarcoma Proliferation and Metastasis.

The local tumor microenvironment (TME) of osteosarcoma (OS) includes several tumor niches that control tumor growth and cell extravasation. Migrasomes are recently discovered extracellular vesicles produced during cell migration. Herein, the results show OS cell production of migrasomes in vivo and in vitro.

Interfacial Structural Transformation for the Synthesis of Lead-Free Double Perovskite Nanocrystals.

Lead-free halide double perovskite nanocrystals have emerged as one of the most promising alternatives to lead halide perovskite nanocrystals due to their non-toxicity, high stability, and outstanding optoelectronic properties. However, conventional synthesis methods often result in impurities due to increased constituent elements. In this study, an efficient water-oil biphasic interface-driven approach is introduced for synthesizing lead-free double perovskite nanocrystals, enabling controlled structural transformations from 0D to 2D and 3D structures.

Mitigating Strain Localization via Stabilized Phase Boundaries for Strengthening Multi-Principal Element Alloys.

Multi-principal element alloys (MPEA) demonstrate exceptional stability during rapid solidification, making them ideal candidates for additive manufacturing and other high-design-flexibility techniques. Unexpectedly, MPEA failure often mimics that of conventional metals, with strain localization along phase or grain boundaries leading to typical crack initiation. Most strategies aim at reducing strain localization either suppress the formation of high-energy sites or dissipate energy at crack tips to enhance toughness, rarely achieving a synergy of both.

Novel Clean-Background Coffee Ring SERS Platform for Trace Toxin Analysis.

Biotoxins, known for their ubiquity and potent toxicity, pose significant threats to ecosystems and human health, highlighting the urgency the development of robust detection methods. Despite the significant potential of surface-enhanced Raman spectroscopy (SERS) in analytical science, challenges emerge when dealing with low Raman active molecules, such as biotoxins. Our study addresses this by introducing an innovative, ultrasensitive, clean-background coffee ring SERS (CC-SERS) platform, specifically designed for the detection of neo-saxitoxin (neo-STX), saxitoxin (STX), microcystin-LR (MC-LR), and microcystin-RR (MC-RR) using the AgNPs@AgO@AgCl coffee rings.

Golgicide A induces pyroptosis of lung cancer stem cells by regulating dTGN formation via GOLPH3/MYO18A complex.

Background: Lung cancer is a common malignant neoplasm, one of the leading causes of death worldwide. Cancer stem cells (CSCs) drive tumor recurrence, progression, and therapeutic resistance. Thus, targeting CSCs may contribute to lung cancer treatment and improve clinical outcomes.

A novel c.59 C > T variant of the HSD17B10 gene as a possible cause of the neonatal form of HSD10 mitochondrial disease with hepatic dysfunction: a case report and review of the literature.

Background: Pathogenic HSD17B10 gene variants cause HSD10 mitochondrial disease (HSD10 MD), which results in a wide spectrum of symptoms ranging from mild to severe. Typical symptoms include intellectual disability, choreoathetosis, cardiomyopathy, neurodegeneration, and abnormal behavior. This study investigated a novel c.

Microenvironment-responsive NIR-IIb multifunctional nanozyme platform for bacterial imaging and specialized anti-anaerobic bacteria periodontal therapy.

Periodontitis is a chronic inflammatory disease caused by plaque. In order to remove pathogens and promote tissue repair, the following steps need to be taken simultaneously: localizing the diseased area, improving the anaerobic microenvironment, as well as addressing the anti-inflammatory and osteogenic needs. This study aims to address these issues by developing a responsive near-infrared-IIb nanozyme system (DMUP), assembled from lanthanide-doped down-converted nanoparticles and multi-enzymatically active nanozyme.

Evaluation of C-reactive protein levels in patients with penile cancer: a systematic review and meta-analysis.

Background: C-reactive protein (CRP) is an essential biomarker for evaluating penile cancer prognosis. Previous studies have reported conflicting outcomes concerning the correlation between CRP levels and penile cancer prognosis. This study aimed to investigate this relationship by conducting a meta-analysis of published literature.

Pretreatment with oral contraceptives benefit POSEIDON group 1 low prognosis patients during GnRH-antagonist protocol: a propensity score-matched retrospective cohort study.

Background: Poor ovarian response (POR) is a challenging condition in assisted reproduction technology. Oral contraceptives (OCs) are commonly used to suppress gonadotropin hormone release in POR patients to synchronize the development of antral follicles before ovarian stimulation. Nevertheless, the question of whether such approach confers advantageous outcomes has elicited inconclusive results in previous studies.