DLBCL cells with ferroptosis morphology can be detected with a deep convolutional neural network.

It has been demonstrated that diffuse large B-cell lymphoma (DLBCL) is especially sensitive to ferroptosis. Currently, confirming the presence of ferroptosis requires flow cytometry, which is a time consuming and labor-intensive task. Blistering of the cell membrane has been shown to be a ferroptosis-specific morphological change.

Evaluating tumour budding could improve the new grading system for lung adenocarcinoma.

Objectives: To study the prognostic significance of tumour budding (TB) compared with the grading of lung adenocarcinoma (LAC).

Materials And Methods: The postoperative haematoxylin and eosin-stained histological slices of 207 surgically treated LAC patients were retrospectively reviewed by a lung pathologist. Two groups were formed from the cohort: the high-grade TB group (≥10 buds) and low-grade TB group (0-9 buds).

The fungicide propiconazole induces hepatic steatosis and activates PXR in a mouse model of diet-induced obesity.

Propiconazole is a triazole fungicide previously shown to induce triglyceride accumulation in human liver HepaRG cells, potentially via activation of the Pregnane X Receptor (PXR). However, whether propiconazole can disrupt hepatic and whole-body metabolism in vivo is currently unknown. Therefore, we aimed to examine the metabolic effects of propiconazole in the context of metabolic dysfunction-associated steatotic liver disease (MASLD), obesity, and insulin resistance.

Performance of Hemochron ACT-LR and ACT+ Test Cuvettes in Monitoring Low to Moderate Heparin Concentrations: An In Vitro Study.

Objectives: According to the manufacturer, the Hemochron ACT-LR cuvette is designated for heparin concentrations of 0 to 2.5 IU/mL, while the optimal concentration range for the ACT+ cartridge is 1 to 6 IU/mL. We hypothesized that at low to moderate heparin concentrations, the ACT-LR is more reliable than the ACT+.

Magnetic Resonance Imaging in the Assessment of the Risk of Sudden Death in Cardiac Sarcoidosis: What Is Extensive or Significant Late Gadolinium Enhancement?

Background: Cardiac sarcoidosis involves a significant but difficult-to-define risk of sudden cardiac death (SCD). Current guidelines recommend consideration of an implantable cardioverter defibrillator for patients with extensive or significant myocardial late gadolinium enhancement (LGE) on cardiac magnetic resonance imaging. However, extensive/significant LGE is not defined.

University hospital status and gastric cancer mortality - a population-based nationwide study in Finland.

Background: Gastric cancer remains a significant global health issue with rising mortality, despite advancements in treatment. Previous studies suggest that surgery performed in university hospitals may influence surgical outcomes and mortality rates, but evidence on its impact for gastric cancer remains limited. This study investigated whether gastrectomy performed at university hospitals reduce short-term and long-term mortality in Finland.

Defining MRI-based follow-up protocol for primary central nervous system lymphoma.

Background: The optimal follow-up protocol for primary central nervous system lymphoma (PCNSL) is unclear. This is the first study to evaluate the benefit of structured follow-up imaging of PCNSL with respect to the timing of relapse.

Methods: A total of 198 PCNSL patients (57.

Development of a Microphysiological Cartilage-on-Chip Platform for Dynamic Biomechanical Stimulation of Three-Dimensional Encapsulated Chondrocytes in Agarose Hydrogels.

Osteoarthritis (OA) is one of the most prevalent joint diseases globally, characterized by the progressive breakdown of articular cartilage, resulting in chronic pain, stiffness, and loss of joint function. Despite its significant socioeconomic impact, therapeutic options remain limited, largely due to an incomplete understanding of the molecular mechanisms driving cartilage degradation and OA pathogenesis. Recent advances in in vitro modeling have revolutionized joint tissue research, transitioning from simplistic two-dimensional cell cultures to sophisticated three-dimensional (3D) constructs that more accurately mimic the physiological microenvironment of native cartilage.

A Novel Label-Free Method for Efficient Detection of Bisphosphonate Drug Dissolution Rates Using Surface-Enhanced Raman Spectroscopy.

We developed a novel method utilizing surface-enhanced Raman spectroscopy (SERS) with calcium ion-modified silver nanoparticles as the enhancing substrate. This approach enables label-free detection of the dissolution rate of bisphosphonates. This technique is fast, sensitive, and highly reproducible, significantly advancing drug quality control.

Association Between Self-Reported Oral Health and Dental Fear Among Illicit Drug Users in Northern Finland.

Objectives: About 10% of adults in northern Finland have severe dental fear, but there is a lack of detailed knowledge about dental fear among illicit drug users. The aim of the study was to evaluate the prevalence of dental fear and its association with self-reported oral health and health behavior as well as background factors of the customers of substance abuse services living in the region of Oulu, Northern Ostrobothnia, Finland.

Materials And Methods: One hundred seventeen volunteers, either active or former illicit drug users, were interviewed face-to-face about their background factors, health and health behaviors, and use of illicit drugs.

Personalized Profiling of Lipoprotein and Lipid Metabolism Based on 1018 Measures from Combined Quantitative NMR and LC-MS/MS Platforms.

Applications of advanced omics methodologies are increasingly popular in biomedicine. However, large-scale studies aiming at clinical translation are typically siloed to single technologies. Here, we present the first comprehensive large-scale population data combining 209 lipoprotein measures from a quantitative NMR spectroscopy platform and 809 lipid classes and species from a quantitative LC-MS/MS platform.

Antenatal socioeconomic status of childhood family and the risk of pain spreading (ROPS) in early and mid-adulthood - a descriptive study from the northern Finland birth cohort 1966.

Objective: The Risk of Pain Spreading (ROPS) is a six-item tool capturing key data-driven prognostic factors for chronic pain and its spreading. Higher values on the ROPS indicate a higher risk. Early factors potentially associated with the ROPS are unknown.

Maternal vitamin B12 during pregnancy and schizophrenia in offspring.

Background: Maternal nutritional deficiency is linked with several adverse outcomes in offspring but the link between maternal vitamin B12 levels and offspring schizophrenia remains unexplored.

Methods: In this nationwide population-based nested case-control design, 1145 schizophrenia cases were born between 1987-1997 and diagnosed by 2017 and each case were matched with a control. Maternal vitamin B12 levels during the first and early second trimesters of pregnancy were measured using chemiluminescence microparticle immunoassay from maternal sera.

Associations of step accelerations and cardiometabolic risk markers in early adulthood.

Physical activity (PA) has a positive effect on risk factors related to cardiometabolic health yet amount of PA and time of onset is unclear. Therefore, we investigated the relationship of PA estimates and cardiometabolic risk factors in a large healthy population of an understudied age group of young adults using a standard gravity-based method on body adiposity and risk markers. In 856 (532 women, 324 men, 32-35 years) subjects we evaluated the association of PA and cardiometabolic risk factors in early adulthood.

Understanding lipidomics associations and the lipoprotein-related caveats in population epidemiology.

Mass spectrometry lipidomics is becoming customary to analyse serum/plasma samples in epidemiology. The measurables are molecular constituents of lipoprotein particles, but very little is known on the consequences of adjusting lipidomics data with lipoprotein measures. We studied two population cohorts with 5,657 and 2,036 participants.

Comparison of balloon catheter, oral misoprostol, or combination of both for cervical ripening in late-term and post-term nulliparous women: A Finnish randomized controlled multicenter pilot trial.

Introduction: Nulliparous women beyond term have high rates of induction failure. The aim of this study was to compare delivery outcomes for balloon catheter, misoprostol, and combination of both in nulliparous late- and post-term women with unfavorable cervices. We intended to explore whether the combination strategy has lower cesarean section rate and is as safe as either method alone.

Cognition, Depression, Pain, and Exercise Motives as Predictors of Longitudinal Profiles of Physical Activity During a Seven-Year Follow-Up Among Older Adults.

This study investigated longitudinal physical activity (PA) profiles over 7 years in the Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER). Cognition, depression, pain, and PA motives were included as determinants of the PA profiles. The 1259 participants, aged 60-77 years at baseline, were randomized into either a control group receiving general health advice, or an intervention group offered a comprehensive 2-year multidomain intervention including physical exercise, diet advice, cognitive training, and vascular risk factor management.

Clinical features and treatment of stroke-like episodes in mitochondrial disease: a cohort-based study.

Background: Stroke-like episode (SLE) is a subacute evolving brain syndrome in patients with primary mitochondrial diseases. Despite previous research, the understanding of the clinical spectrum, treatment, and outcomes of mitochondrial SLEs is far from complete. In this single centre study, we report the clinical symptoms and radiological findings as well as the medical treatment and outcomes of SLEs in patients with mitochondrial disease.

TissueGene-C induces anti-inflammatory activity and M2 macrophage polarization via activation of prostaglandin E signaling.

Background And Aim: Osteoarthritis (OA) is the most common form of degenerative joint disease that commonly affects the knees, hips and hands. OA is a mechano-inflammatory disease characterized by low-grade inflammation, which results in breakdown of the cartilage extracellular matrix within joints, leading to pain, stiffness and inflammation. TissueGene-C (TG-C) is a cell and gene therapy investigational drug for treating knee OA that comprises human allogeneic chondrocytes and an irradiated modified cell line stably expressing transforming growth factor beta 1 (TGF-β1).

tREatment of trIaNgular FibrOcaRtilage ComplEx Ruptures (REINFORCER): protocol for randomised, controlled, blinded, efficacy trial of triangular fibrocartilage complex tears.

Introduction: Triangular fibrocartilage complex (TFCC) tear is often considered to be the cause of ulnar wrist pain. The primary treatment typically involves non-operative methods; however, in cases of persistent symptoms, operative intervention has been proposed as a viable option. Depending on the tear's morphology, treatment may involve debridement (central or radial tear) or repair (peripheral tear).

Common lipidomic signatures across distinct acute brain injuries in patient outcome prediction.

Lipidomic alterations have been associated with various neurological diseases. Examining temporal changes in serum lipidomic profiles, irrespective of injury type, reveals promising prognostic indicators. In this longitudinal prospective observational study, serum samples were collected early (46 ± 24 h) and late (142 ± 52 h) post-injury from 70 patients with ischemic stroke, aneurysmal subarachnoid hemorrhage, and traumatic brain injury that had outcomes dichotomized as favorable (modified Rankin Scores (mRS) 0-3) and unfavorable (mRS 4-6) three months post-injury.

Bioinformatic characterization of ENPEP, the gene encoding a potential cofactor for SARS-CoV-2 infection.

Research on SARS-CoV-2, the viral pathogen that causes COVID-19, has identified angiotensin converting enzyme 2 (ACE2) as the primary viral receptor. Several genes that encode viral cofactors, such as TMPRSS2, NRP1, CTSL, and possibly KIM1, have since been discovered. Glutamyl aminopeptidase (APA), encoded by the gene ENPEP, is another cofactor candidate due to similarities in its biological role and high correlation with ACE2 and other human coronavirus receptors, such as aminopeptidase N (APN) and dipeptidyl peptidase 4 (DPP4).

Patients' Experiences of Healthcare Professionals' Competence in Digital Counselling in Healthcare Settings-A Qualitative Systematic Review.

Aim: To critically appraise and synthesise qualitative evidence about patients' experiences of healthcare professionals' competence in digital counselling in healthcare settings.

Design: A qualitative systematic review.

Methods: The review followed the Joanna Briggs Institute methodology for systematic reviews of qualitative evidence.