Evaluation of a new automated, enzymatic inulin assay using D-fructose dehydrogenase.

Background: Inulin clearance (Cin) is widely considered to be a gold-standard assessment of glomerular filtration rate (GFR). Although inulin concentrations are commonly determined by the anthrone method, this method has several disadvantages, including a hazardous heating procedure using a strong acid, as well as a nonspecific reaction and being influenced by other saccharides such as glucose.

Method: Here, we report the development of a new automated, enzymatic inulin assay.