[PFAS and 5G: what can we tell patients about these two current scientific topics?].

Issues such as PFAS and 5G are regularly in the media spotlight like this of Nutri-Score which is discussed in a second article. The model of the physician possessing all medical knowledge is evolving. Patients now come to consultations with information from a variety of sources, some of which are exhaustive, some of which are not.

[Nutri-Score : what can we tell patients about this current scientific topic?].

Issues such as the Nutri-score is regularly in the media spotlight like those of PFAS and 5G which are discussed in a second article. The model of the physician possessing all medical knowledge is evolving. Patients now come to consultations with information from a variety of sources, some of which are exhaustive, some of which are not.

[Acute abdominal pain in general practice].

Acute abdominal pain (AAP) represents the 9th most common complaint in general practice. The etiologies are multiple: a third are non-specific, but around 10% require hospital treatment. An accurate history enables a correct diagnosis in 76% of cases, and abdominal palpation can detect a possible surgical abdomen.

[Prostate cancer screening : to be recommended in 2024?].

The Prostate Specific Antigen (PSA) blood test is still the only screening tool for prostate cancer. It is recommended between the ages of 50 and 69 as part of a shared decision making process between a patient and his or her doctor using a decision aid, as the test carries a significant risk of overdiagnosis. If a patient wishes to be screened, either because he is at higher risk, or because he places greater importance on a modest reduction in cancer-related mortality, the frequency of screening depends on his age, family history, and whether he is part of a high-risk group.

[Red eye : common ocular pathologies in outpatient care].

Red eye is a common condition characterized by eye redness, often accompanied by itching, burning, pain, or discharge. This condition can be caused by a multitude of factors, ranging from minor irritations to severe infections. Red eyes are a frequent complaint in outpatient practice.

[Knee pain in primary care medicine : diagnostic and therapeutic approach].

Knee pain is one of the most common musculoskeletal complaints in primary care medicine. The leading causes of knee pain are osteoarthritis and musculoskeletal disorders. A careful history and a rigorous clinical examination usually enable a diagnosis to be made without the need for further investigations.

[Hand infections : when to consult a hand surgeon ?].

The hand is an anatomical site frequently affected by infection due to its central role in all daily activities. It is therefore regularly the site of wounds (contused, penetrating, insect stings, bites, etc.), all of which are potential entry points for infections.

Analyse des conditions à l'utilisation de l' par des équipes de promotion de la santé : résultats d'une étude de cas multiples en région bruxelloise (Belgique).

Introduction: L'évaluation réaliste est de plus en plus plébiscitée dans le champ de la promotion de la santé. Pourtant, cette démarche d'évaluation est encore très peu utilisée par, ou même avec, des équipes de terrain, ce qui vient limiter sa portée pour les pratiques de terrain. Notre étude propose d'explorer les conditions favorables à la pratique de l'évaluation réaliste par des équipes de promotion de la santé.

Évaluation de la mise en œuvre des missions de service sanitaire en santé sexuelle en Île-de-France.

Contexte: Le service sanitaire français, réalisé par des étudiants en santé (3 année), est un programme de promotion de la santé (PS) récent qui est encore peu évalué.

Objectif: Cette étude s'est intéressée au service mis en place dans la filière médecine à l'université Paris Cité, sur la thématique santé sexuelle. L'objectif de l'étude est d'évaluer des interventions réalisées par les étudiants en termes pédagogiques et en termes de bonnes pratiques en PS, sur les plans suivants : éthique, qualité du contenu et communication.

Gapless assembly of complete human and plant chromosomes using only nanopore sequencing.

The combination of ultra-long (UL) Oxford Nanopore Technologies (ONT) sequencing reads with long, accurate Pacific Bioscience (PacBio) High Fidelity (HiFi) reads has enabled the completion of a human genome and spurred similar efforts to complete the genomes of many other species. However, this approach for complete, "telomere-to-telomere" genome assembly relies on multiple sequencing platforms, limiting its accessibility. ONT "Duplex" sequencing reads, where both strands of the DNA are read to improve quality, promise high per-base accuracy.

Perceptions of readiness for independent practice among graduating orthopedic surgery residents in Ontario in the last 30 years.

Background: There is increasing concern regarding the lack of physicians and underresourcing of the medical system in Canada. The training of orthopedic surgeons has emerged as an area of particular concern. The purpose of this study was to gain insight into the outcomes of graduates of orthopedic surgery residency programs in Ontario in the last 30 years.

The importance of interdisciplinary collaboration in advanced therapy of odontogenic cysts: A 31 Month follow-up case report.

In this case report, we present the treatment of a 39-year-old male patient with a bilateral maxilla cyst diagnosed as an additional finding ont he X-ray. Both conservative dentistry treatments and oral surgical procedures were carried out using state-of-the-art materials and equipment, and in close collaboration with the other dental specialists. Endodontic treatment of the remaining teeth was performed before the oral surgery treatment.

TDFPS-Designer: an efficient toolkit for barcode design and selection in nanopore sequencing.

Oxford Nanopore Technologies (ONT) offers ultrahigh-throughput multi-sample sequencing but only provides barcode kits that enable up to 96-sample multiplexing. We present TDFPS-Designer, a new toolkit for nanopore sequencing barcode design, which creates significantly more barcodes: 137 with a length of 20 base pairs, 410 at 24 bp, and 1779 at 30 bp, far surpassing ONT's offerings. It includes GPU-based acceleration for ultra-fast demultiplexing and designs robust barcodes suitable for high-error ONT data.

Emergency department use before cancer diagnosis in Ontario, Canada: a population-based study.

Background: Although suspicions of cancer may be raised in patients who visit the emergency department, little is known about emergency department use before a cancer diagnosis. We sought to describe emergency department use among patients in Ontario within the 90 days before confirmed cancer diagnosis and to evaluate factors associated with this emergency department use.

Methods: We conducted a retrospective, population-based study of patients aged 18 years or older who had a confirmed cancer diagnosis in Ontario from 2014 to 2021 using linked administrative databases.

Resolving 'vascular parkinsonism' -COL22A1 as a genetic adult-onset leukoencephalopathy.

We describe an elderly woman meeting criteria for vascular parkinsonism/dementia associated with a likely pathogenic variant in the COL22A1 gene, supported by functional experiments in a zebrafish model. We suggest that white matter hyperintensities may not represent small-vessel ischemic disease and that, if confirmed by further observations, COL22A1 may be included among the adult-onset leukoencephalopathies mislabeled as "vascular parkinsonism."

Third generation sequencing analysis detects significant differences in duodenal microbiome composition between functional dyspepsia patients and control subjects.

Background: Functional dyspepsia (FD) is a multifactorial disorder as its development may be based on several different pathophysiological mechanisms. Interaction of gut microbiome with the host has been proposed as a potential mechanism involved in the disease's pathogenesis.

Aim/methods: We aimed to characterize microbiome profiling on duodenal luminal content (DLC) of FD patients and compare it to that of controls (CG) and patients with irritable bowel syndrome (IBS).

K-mer analysis of long-read alignment pileups for structural variant genotyping.

Accurately genotyping structural variant (SV) alleles is crucial to genomics research. We present a novel method (kanpig) for genotyping SVs that leverages variant graphs and k-mer vectors to rapidly generate accurate SV genotypes. We benchmark kanpig against the latest SV benchmarks and show single-sample genotyping concordance of 82.

Clinical RNA sequencing clarifies variants of uncertain significance identified by prior testing.

Purpose: Sequencing-based genetic testing often identifies variants of uncertain significance (VUS) or fails to detect pathogenic variants altogether. We evaluated the utility of RNA sequencing (RNA-seq) to clarify VUS or identify missing variants in a clinical setting.

Methods: Over a 2-year period, genetics providers at a single institution referred 26 cases for clinical RNA-seq.

ONT sequencing identifies a high prevalence of sensitive, triple mutant and single mutant parasites within an ANC population in Nigeria.

Background: Malaria in pregnancy is a major public health issue, particularly among vulnerable populations in malaria-endemic sub-Saharan African countries. To mitigate its risks, WHO recommends sulphadoxine-pyrimethamine (SP) for chemoprevention and artemisinin-based combination therapy (ACT) to treat uncomplicated malaria. These interventions have helped to alleviate the risk associated with malaria in pregnancy; however, in the context of the emergence of SP- and ACT-resistant , maintained efficacy is under threat.

Evaluating culture-free targeted next-generation sequencing for diagnosing drug-resistant tuberculosis: a multicentre clinical study of two end-to-end commercial workflows.

Background: Drug-resistant tuberculosis remains a major obstacle in ending the global tuberculosis epidemic. Deployment of molecular tools for comprehensive drug resistance profiling is imperative for successful detection and characterisation of tuberculosis drug resistance. We aimed to assess the diagnostic accuracy of a new class of molecular diagnostics for drug-resistant tuberculosis.