Nanopore-Based Sequencing of the Full-Length Transcriptome of Male and Female Cleavage-Stage Embryos of the Chinese Mitten Crab ().

The cleavage stage plays a crucial role in embryo development, characterized by a swift surge in cell proliferation alongside the accurate genetic material transmission to offspring. To delve into the characteristics of sex development during the cleavage stage of embryos, we generated the full-length transcriptome of male and female cleavage-stage embryos using Oxford Nanopore Technologies (ONT). Notably, this investigation represents the first sequencing effort distinguishing between genders in embryos.

Identification of a terpene synthase arsenal using long-read sequencing and genome assembly of Aspergillus wentii.

Background: Fungi are talented producers of secondary metabolites with applications in the pharmaceutical and agrochemical sectors. Aspergillus wentii CBS 141173 has gathered research interest due to its ability to produce high-value norditerpenoid compounds, including anticancer molecules. In this study, we aimed to expand the genomic information available for A.

The impact of COVID-19 on pancreaticoduodenectomy outcomes in a hepatopancreatobiliary centre of excellence.

Background: At the beginning of the COVID-19 pandemic, access to "planned" surgical care was restricted as the health care system responded to the coronavirus. We hypothesized that the pandemic resulted in diagnostic and therapeutic delays, leading to stage migration among patients with malignancies treated with a Whipple procedure.

Methods: This study is a retrospective review of adults who underwent surgical exploration for a planned pancreaticoduodenectomy for malignancy at St.

Incidence and timing of postoperative complications after total hip and knee arthroplasty.

Background: Follow-up protocols after total hip or knee arthroplasty (THA or TKA, respectively) have little uniformity, which can lead to emergency department (ED) visits for postoperative complications. We sought to determine the incidence and timing of postoperative complications after THA or TKA.

Methods: We conducted a population-based retrospective cohort study of all adults in Ontario who underwent primary THA or TKA between 2010 and 2019.

Construction and analysis of telomere-to-telomere genomes for 2 sweet oranges: Longhuihong and Newhall (Citrus sinensis).

Background: Sweet orange (Citrus sinensis Osbeck) is a fruit crop of high nutritional value that is widely consumed around the world. However, its susceptibility to low-temperature stress limits its cultivation and production in regions prone to frost damage, severely impacting the sustainable development of the sweet orange industry. Therefore, developing cold-resistant sweet orange varieties is of great necessity.

Chromosome-level assembly of the genome.

is an important medicinal herb in the Lamiaceae family. This species lacks corresponding genomic resources, which significantly limits the study of its active compound synthesis pathways, breeding practices, and assessment of natural genetic variations. We assembled the chromosomal-level genome of using Oxford Nanopore (ONT) technology and Hi-C sequence.

Gap-free telomere-to-telomere haplotype assembly of the tomato hind (Cephalopholis sonnerati).

The tomato hind (Cephalopholis sonnerati) is an emerging economically important grouper in recent years. With the increasing maturity of sequencing technologies and assembly methodologies, a higher quality reference genome has become both accessible and necessary. In this study, we present two telomere-to-telomere (T2T) gap-free haplotype assemblies of the tomato hind with lengths of 1039.

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Introduction: Primary liver cancer (PLC) occupied the 6th and 3rd place in the world respectively in terms of incidence and mortality in 2020. The objective of this work was to study the survival and prognostic factors of primary liver cancer from 2014 to 2020 in Cotonou.

Materials And Methods: This retrospective cohort study included 150 patients registered with primary liver cancer, in the database of the Cotonou Cancer Registry, over a seven-years period from 01/01/2014 to 12/31/2020.

Ship collision risk threatens whales across the world's oceans.

After the near-complete cessation of commercial whaling, ship collisions have emerged as a primary threat to large whales, but knowledge of collision risk is lacking across most of the world's oceans. We compiled a dataset of 435,000 whale locations to generate global distribution models for four globally ranging species. We then combined >35 billion positions from 176,000 ships to produce a global estimate of whale-ship collision risk.

[Pregabalin in prisons: recommendations for the general practitioner].

The misuse of pregabalin (Lyrica) has emerged as a common problem in recent years, particularly among vulnerable populations. This reality is reflected in the prison environment, where pregabalin is subject to abuse and trafficking. In this article, we detail the issues surrounding this misuse and our practical strategy for tackling it.

[Planning hospital-focused training in internal medicine at the CHUV].

Switzerland already suffers from the long-predicted shortage of physicians. Moreover, the latter often face long and meandering postgraduate training. At the beginning of their career, most doctors work on internal medicine hospital wards, even though their future may just as well lie in an outpatient or inpatient setting, in general internal medicine or in other disciplines.

[Promoting physical activity in everyday life: the role of health care professionals].

Physical activity (PA) promotion has become a central objective of Public Health Organisations, due to its long-lasting global benefits on health. The 2023 recommendations recommend at least 150 minutes of moderate physical activity or 75 minutes of intense physical activity per week for adults. New approaches focus on physical exercise as an integral part of daily life.

[Sustainability and choice of inhaled treatment for obstructive pulmonary diseases].

Inhaled treatments are essential for obstructive lung diseases such as asthma and chronic obstructive pulmonary disease (COPD). However, pressurized metered-dose inhalers (pMDI) use propellant gases, contributing to greenhouse gas (GHG) emissions and thereby to climate change. The use of dry powder inhalers (DPI) or soft mist inhalers (SMI) when clinically appropriate is a way to reduce these emissions.

[Sickle cell crisis: inpatient management].

Sickle cell anemia is the world's most common monogenic disease, with a steady rise in the number of cases also in Switzerland. It presents as regenerative anemia and recurrent vaso-occlusive episodes, which can impact all organs. Acute and chronic complications increase morbidity and mortality, significantly reducing life expectancy compared to the general population.