Effects of sperm direction in Piezo-ICSI on oocyte survival, fertilization, embryo development and implantation ability in humans: A preliminary study.

Aims: The objective of this study was to investigate the effect of the head-first or tail-first injection of sperm into the cytoplasm by Piezo-ICSI (PICSI) on oocyte survival, fertilization, embryo development and implantation ability in humans.

Methods: We retrospectively investigated 632 mature oocytes retrieved from 152 infertile patients who attended our PICSI-ET program at the Niji Clinic between October 2010 and January 2014. Of these, 342 mature oocytes retrieved from 75 patients were injected with sperm head first, and 290 mature oocytes retrieved from 77 patients were injected with sperm tail first into the cytoplasm.

Erratum to: Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm.

Erratum to: J Assist Reprod Genet (2015) 32:1827–1833, DOI 10.1007/s10815-015-0597-9. The authors would like to apologize for accidentally making a mistake in the inner and outer diameter calculation of the Piezo-ICSI micropipettes.

Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm.

Purpose: The purposes of the present study are to assess the clinical efficiency of Piezo-intracytoplasmic sperm injection (ICSI) and to improve the Piezo-ICSI method for human oocytes.

Methods: We examined three ICSI methods to determine their clinical efficiency by comparing the survival, fertilization, good-quality day-3 embryo, pregnancy, and live birth rates. The three ICSI methods tested were conventional ICSI (CI) (using beveled spiked micropipettes with a wall thickness of 1 μm), conventional Piezo-ICSI (CPI) (using flat-tipped micropipettes with a wall thickness of 0.