Combined ethanol and radiofrequency ablation for the elimination of focal atrial tachycardia originating from the Marshall bundle.

Introduction And Importance: Atrial tachycardias (AT) originating from the Marshall bundle (MB) are rare and present significant challenges in diagnosis and management. The authors present the case of a 29-year-old male with recurrent AT successfully treated with a combined ethanol and radiofrequency ablation approach. This case highlights the effectiveness of this dual ablation strategy in resolving AT originating from the MB, contributing valuable insights into managing complex AT cases.

[Problem of mineral insufficiency at chronic pancreatitis in dependence on age].

The high incidence of chronic pancreatitis (CP) which arise at young people of working age, the development of serious complications are pushing for the development of new diagnostic methods and the search for effective ways to treat this disease. In this regard, a relevant and promising direction is the further study of the mechanisms of pathogenesis and the formation of trophological (including mineral) deficiency in CP, followed by the development of comprehensive programs for their correction. is to study mineral status of patients with chronic pancreatitis, depending on their age.

GHSR DNA hypermethylation is a common epigenetic alteration of high diagnostic value in a broad spectrum of cancers.

Identification of a single molecular trait that is determinant of common malignancies may serve as a powerful diagnostic supplement to cancer type-specific markers. Here, we report a DNA methylation mark that is characteristic of seven studied malignancies, namely cancers of lung, breast, prostate, pancreas, colorectum, glioblastoma and B cell chronic lymphocytic leukaemia (CLL) (n = 137). This mark was defined by substantial hypermethylation at the promoter and first exon of growth hormone secretagouge receptor (GHSR) through bisulfite pyrosequencing.

The prevalence of and knowledge about tobacco use among physicians in the Odessa region, Ukraine.

We investigated prevalence of and knowledge about tobacco use among physicians, and their counselling of patients in the Odessa region (Ukraine). Paediatricians (40), family doctors (40) and interns (70) were selected from the physician population of the Odessa region. The proportion of smokers was unacceptably high for health care professionals: paediatricians, 32.

Mechanisms of geomagnetic field influence on gene expression using influenza as a model system: basics of physical epidemiology.

Recent studies demonstrate distinct changes in gene expression in cells exposed to a weak magnetic field (MF). Mechanisms of this phenomenon are not understood yet. We propose that proteins of the Cryptochrome family (CRY) are "epigenetic sensors" of the MF fluctuations, i.

Specificity of action of epileptogenic agents in diazepam-tolerant rats.

The development of tolerance in rats by repeated intraperitoneal injection of 0.5 mg/kg diazepam increased the sensitivity of the frontal cortex to the epileptogenic effect of NMDA and kainic acid. The median effective dose of NMDA was shown to decrease under these conditions.

Microarray study of gene expression in uterine leiomyoma.

Unlabelled: Uterine leiomyoma is a most common benign neoplasm in women of reproductive age. It arises from the myometrial compartment of the uterus and may transform in some cases to a malignant phenotype.

Aim: To identify the genes involved in pathogenesis of uterine leiomyoma.

Effects interferon-2alpha on seizures in corasol-induced kindling.

Intracerebroventricular injection of recombinant human interferon-2alpha (100-500 U/rat) decreased the mean effective dose of corasol inducing clonic convulsions in 50% kindled Wistar rats. This effect was more pronounced in delayed periods of kindling and was accompanied by enhancement of epileptiform signs on EEG.

Interleukin-6 -174G/C polymorphism in breast cancer and uterine leiomyoma patients: a population-based case control study.

Unlabelled: A common G/C polymorphism at position -174 of the interleukin-6 (IL-6) gene promoter is thought to affect the expression level of this gene, thus predisposing to a variety of diseases, including tumorigenesis.

Aim: To investigate the association of the -174 G/C IL-6 polymorphism with breast cancer and uterine leiomyoma in Ukrainian population.

Methods: We genotyped the -174 G/C IL-6 polymorphism in a group of healthy Ukrainians by pyrosequencing and compared the results to those previously published for other Caucasian populations.

Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population.

Aim: To identify clinically significant molecular markers of endometrial cancer.

Materials And Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed.

[Histiocytosis, central diabetes insipidus, and pregnancy].

Dysfunction of the hypothalamus causes a significant polymorphism of the clinical symptomatology of diseases of the hypothalamic-pituitary system, associated with an excess or deficiency of pituitary hormones and the corresponding hyper- or hypofunction of the peripheral endocrine glands. In particular, a violation of the integrity of the hypothalamic-neurohypophysial tract, synthesis, transportation, and release of vasopressin leads to disorders of the water balance in the form of central diabetes insipidus. In 15-30% of all cases of diabetes insipidus, its primary (idiopathic) forms are detected.

Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine.

Understanding the molecular epidemiology of tuberculosis (TB) and mutations in genes associated with drug resistance may contribute to the development of appropriate interventions to improve tuberculosis control. A structured questionnaire was used to collect basic epidemiological data from 589 patients with radiologically confirmed TB in the Odessa and Nikolaev regions of the Ukraine in 2003-2004. A non-commercial reverse hybridisation assay and DNA sequencing were used to detect mutations associated with rifampicin and isoniazid resistance.

Are there different mechanisms of synchronization in the course of spike-wave discharges (SWDs) burst development in WAG/Rij rats?

In WAG/Rij rats the pair linear correlation r was calculated for bipolar recordings in fronto-temporal, fronto-occipital and occipito-temporal zones of both hemispheres as well as in paleocerebellar cortex (culmen). It was shown that development of SWD bursts resulted in interhemispheric decreases of correlation between the right occipito-temporal cortical region on one side, and left fronto-temporal on the contralateral side. Towards the end of SWD, we found an increased interhemispheric correlation between left fronto-temporal and right fronto-occipital cortical zones, as well as, between both fronto-temporal zones.

Comparison of long-term results of laparoscopic and endoscopic exploration of common bile duct.

Background: To compare long term results of laparoscopic and endoscopic exploration of common bile duct, to assess post-procedure quality of life.

Materials And Methods: From September 1992 to August 2003, we performed 4058 cholecystectomies, out of which 479 (11.80%) patients had choledocholithiasis.

Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions: implication of 2-oxoglutarate and iron.

Cells exposed to low oxygen conditions respond by initiating defense mechanisms, including the stabilization of hypoxia-inducible factor (HIF) 1alpha, a transcription factor that upregulates genes such as those involved in angiogenesis and glycolysis, which also plays a pivotal role in the regulation of cellular utilization of oxygen and is an essential regulator of angiogenesis in solid tumor and ischemic disorders. Nitric oxide and other inhibitors of mitochondrial respiration prevent the stabilization of HIF-1alpha during hypoxia. In the present study we found that nitric oxide inhibits HIF-1alpha accumulation under low oxygen (1%) conditions.

The effects of L-DOPA and transcranial magnetic stimulation on behavioral reactions in kindled rats.

Acute experiments were performed on rats to produce a model of chronic epileptic activity--pharmacological kindling by repeated doses of picrotoxin (1.0-1.2 mg/kg, i.

The effects of electrical stimulation of the paleocerebellar cortex on penicillin-induced convulsive activity in rats.

Studies in Wistar rats in conditions of free behavior showed that low-frequency stimulation of the paleocerebellar cortex (nodulus, uvula) (10-12 Hz, 0.5 msec) was accompanied by activation of spike discharges induced by systematic application of benzylpenicillin sodium (3,000,000 IU/kg). Facilitation of the formation of ictal discharges was also seen.

[Role of nitric oxide in tumor growth].

This review article will analyze the role of nitric oxide in antiblastomous organism resistance, in particular some mechanisms of NO-mediated apoptosis in different cells and NO involvement in etiological mechanisms as well as tumor growth promotion. The possible mechanisms of nitric oxide dual effect are discussed. The data about NO as a mediator in different methods of cancer treatment are given.

[Effects of L-dopa and transcranial magnetic stimulation on behavioral reactions in kindled rats].

In acute experiment in rats, the chronic epileptogenesis was reproduced in the form of pharmacological kindling induced via repeated picrotoxin administrations (1.0-1.2 mg/kg, intraperitoneally).

[Effect of paleocerebellar cortex electrical stimulation on generalized penicillin-induced seizure activity in rats].

It was shown that low-frequency electrical stimulation (ES) (10-12 Hz, 0.5 ms) of paleocerebellar cortex (nodulus, uvula) is followed by activation of spike discharges which were induced via i.p.

[Antioxidant and anti-inflammatory effect of "vitalong" in hyperlipidemia in rats].

In experiment on model of the lipidemia in rats the efficiency of new antioxidant drug "Vitalong" was studied. The researches have shown that the drug brakes derivation of free radicals, increases activity of a superoxide scavenger, has antiinflammatory and adaptogenic operation. Vitalong prevents activation of serumal elastasa, locking thus destructive link of the atherogenesis.

[Effects of hypogeomagnetic fields on the structural-functional activity of rat cerebral cortex].

Structural functional organization state of brain cortex has been determined by histological, hystochemical and morfometrical methods from 104 animals, which were exposed by long stay of hypogeomagnetic field. It is found the disturbance of brain cortex micro-circulation; the disturbance of the enzymes activity of oxidate-reductive types, analogous to hypoxic; monthly chronobiological cycle of neurons content of basic structural functional types replace by changes of this index. The authors suppose that as a result the desynchronization of brain cortex activity as functional system appear.

The significance of ante- and perinatal periods for formation of risk of sudden infant death syndrome.

The results of the research, which are carried out according to two international programs on studying and prevention of the sudden infant death syndrome (SIDS), are submitted in the article. The features of ante- and perinatal periods, characteristics of obstetric history of the mothers of deceased babies are analyzed, the relative risk of SIDS development is estimated at the influence of a complex of factors. High parity, young age of mother at the time of delivery, poor antenatal care, preterm delivery, intrauterine fetal growth retardation, fast delivery, alcohol and coffee abuse by a mother, smoking during pregnancy, early change of the child's feeding from breast to bottle are referred to the most significant risk factors of SIDS stipulated by the unfavorable course of pregnancy and delivery.