A genome-scale metabolic model of a globally disseminated hyperinvasive M1 strain of .

is responsible for a range of diseases in humans contributing significantly to morbidity and mortality. Among more than 200 serotypes of , serotype M1 strains hold the greatest clinical relevance due to their high prevalence in severe human infections. To enhance our understanding of pathogenesis and discovery of potential therapeutic approaches, we have developed the first genome-scale metabolic model (GEM) for a serotype M1 strain, which we name iYH543.

Association between host defence peptide IDR-1002 and ciprofloxacin: Effects on human dental pulp cells.

To evaluate the effects of the association of host defence peptide IDR-1002 and ciprofloxacin on human dental pulp cells (hDPSCs). hDPSCs were stimulated with ciprofloxacin and IDR-1002. Cell viability (by MTT assay), migration capacity (by scratch assay), production of inflammatory and anti-inflammatory mediators by hDPSCs (RT-PCR) and osteogenic differentiation (alizarin red staining) were evaluated.

The salivary virome during childhood dental caries.

While many studies have examined the bacterial taxa associated with dental caries, the most common chronic infectious disease globally, little is known about the caries-associated virome. In this study, the salivary viromes of 21 children with severe caries (>2 dentin lesions) and 23 children with healthy dentition were examined. 2,485 viral metagenome-assembled genomes (vMAGs) were identified, binned, and quantified from the metagenomic assemblies.

Interdisciplinary treatment for an adolescent with fusion of the left maxillary lateral and central incisors: A clinical report.

The interdisciplinary treatment provided for a 12-year-old boy with fusion of his left central and lateral incisors and concomitant distal space loss is described. His treatment was initiated with orthodontic therapy followed by endodontic treatment of the fused tooth. A conservative zirconia and porcelain cantilevered bonded fixed dental prosthesis was then completed to replace his left lateral incisor.

Interdisciplinary prosthetic rehabilitation following bilateral maxillectomy with total upper lip and unilateral zygoma resection: A clinical report.

This clinical report describes the design and fabrication of an implant-retained and soft-tissue-supported obturator prosthesis and a magnetic retained midfacial prosthesis for a patient with a midfacial defect following bilateral total maxillectomy, removal of the left zygoma, and excision of the entire upper lip. The patient underwent multiple head and neck surgeries for 4 separate primary oral cancers, 1 recurrence, and 1 pulmonary metastasis. The definitive obturator prosthesis was retained by 1 zygomatic implant with soft-tissue support and retention.

Student perception and assessment outcomes using a pilot condensed format ("boot camp") in a Fixed Prosthodontics course.

Introduction: The purpose of this study was to assess student perception as well as course outcomes (final written examination grades) when using a pilot condensed format in an undergraduate Fixed Prosthodontics course, which was implemented as a response to the global pandemic of Covid-19.

Materials And Methods: The course was delivered in a condensed format, with a total of 28 direct contact hours in the Simulation Clinic within a 4 day period. Asynchronous remote learning was employed for the didactic portion of the course.

Gender diversity and senior leadership in academic dentistry: Female representation at the dean position in the United States.

Purpose/objectives: The purpose of this study was to calculate the male to female dean ratio in the US dental schools, assess whether the dean's gender is affected by specific school characteristics (such as geographic location or class size), and determine whether the dean's gender affects the percent of women in the school leadership team.

Methods: Each US school's website was reviewed to capture the name and gender of each dean and others holding assistant, associate or vice dean positions. All data were entered in an Excel database (Microsoft Excel 2016) and analyzed using a statistical analysis software program (IBM SPSS Statistics, v26; IBM Corp).

Antibacterial, ester-free monomers: Polymerization kinetics, mechanical properties, biocompatibility and anti-biofilm activity.

Objectives: Quaternary ammonium (QA) methacrylate monomers have been extensively investigated and demonstrate excellent antibacterial properties. However, the presence of ester bonds makes them prone to degradation in the oral cavity. In this study, ester-free QA monomers based on meth-acrylamides were synthesized and screened for polymerization kinetics, mechanical properties and antibacterial effects.

Engineering Microvascular Networks in LED Light-Cured Cell-Laden Hydrogels.

The success of tissue engineering inevitably depends on the fabrication of tissue constructs that can be vascularized and that anastomose with the host vasculature. In this report, we studied the effects of light-emitting diode (LED) photopolymerized gelatin methacryloyl hydrogels (GelMA), encapsulated with stem cells from the apical papilla (SCAP) and human umbilical vein endothelial cells (HUVECs), in promoting vasculature network formation as a function of hydrogel physical and mechanical properties, as well as total cell density. Lithium acylphosphinate (LAP) was used as the photoinitiator in concentrations of 0.

A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry.

Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix.

Photopolymerization of cell-laden gelatin methacryloyl hydrogels using a dental curing light for regenerative dentistry.

Unlabelled: Photopolymerized hydrogels, such as gelatin methacryloyl (GelMA), have desirable biological and mechanical characteristics for a range of tissue engineering applications.

Objective: This study aimed to optimize a new method to photopolymerize GelMA using a dental curing light (DL).

Methods: Lithium acylphosphinate photo-initiator (LAP, 0.

Effects of periodontitis on the development of asthma: The role of photodynamic therapy.

To evaluate whether periodontitis modulates lung inflammation in an experimental model of asthma as well as the photodynamic therapy (PDT) is associated with a reduction of lung inflammation. Seventy-two BALB/c male mice (~2 months) were randomly divided into 8 groups (n = 9): Basal, Periodontitis (P), P+PT, P+PT+PDT, Asthma (A), A+P, A+P+PT, and A+P+PT+PDT. Periodontitis was induced by using the ligature technique and asthma was induced by ovalbumin (OVA).

Unraveling the mechanistic effects of electric field stimulation towards directing stem cell fate and function: A tissue engineering perspective.

Electric field (EF) stimulation can play a vital role in eliciting appropriate stem cell response. Such an approach is recently being established to guide stem cell differentiation through osteogenesis/neurogenesis/cardiomyogenesis. Despite significant recent efforts, the biophysical mechanisms by which stem cells sense, interpret and transform electrical cues into biochemical and biological signals still remain unclear.

Biomaterials for Craniofacial Bone Regeneration.

Functional reconstruction of craniofacial defects is a major clinical challenge in craniofacial sciences. The advent of biomaterials is a potential alternative to standard autologous/allogenic grafting procedures to achieve clinically successful bone regeneration. This article discusses various classes of biomaterials currently used in craniofacial reconstruction.

Dental Impression Materials and Techniques.

Dental impression making is the process of creating a negative form of the teeth and oral tissues, into which gypsum or other die materials can be processed to create working analogues. Contemporary dentistry generates new information every year and digital dentistry is becoming established and influential. Although dentists should stay abreast of new technologies, some of the conventional materials and time-tested techniques remain widely used.

Qualitative evaluation of a physical activity health promotion programme for people with intellectual disabilities in a group home setting.

Background: There is a lack of health promotion programming designed to change the physical activity environment of the group home setting. The Menu-Choice programme assists staff in creating physical activity goals alongside residents with intellectual disabilities and provides strategies to incorporate activity into the group home schedule. The purpose of this study was to complete a process evaluation of Menu-Choice utilizing qualitative methods.

Formative evaluation on a physical activity health promotion program for the group home setting.

Physical inactivity and high rates of chronic conditions is a public health concern for adults with intellectual disability. Few health promotion programs target the group home setting which is the pre-dominant form of residential accommodation for persons with intellectual disability. A process evaluation of a physical activity health promotion program, Menu-Choice, was conducted with five group home sites for adults with intellectual and developmental disabilities.

Mutans streptococci genetic strains in children with severe early childhood caries: follow-up study at one-year post-dental rehabilitation therapy.

Background: Genotypic strains of cariogenic mutans streptococci (MS) may vary in important virulence properties. In previous published studies, we identified 39 MS strains from pediatric patients undergoing full-mouth dental rehabilitation, including the removal and/or repair of carious lesions and application of antimicrobial rinse and fluoride varnish.

Objectives: The objectives of this current 1-year follow-up study are to assess the variability of MS strains that occur at 1-year post-rehabilitation and characterize the xylitol-resistance properties of MS strains that predominate.

Critical appraisal: preheating composites.

Resin composite is the most commonly used material for direct placement restorations. In particular, composite is now used more frequently than amalgam for direct posterior restorations. However, resin composite still suffers from some disadvantages, including the use of an adhesive interfacial bond that degrades with time, moisture, and function in the mouth, and certain key mechanical properties (e.

Comparison of various resin composite shades and layering technique with a shade guide.

Purpose: The purpose of this study was to compare the final shade of different shades of composites (enamel shades) over a white backing (WB) and black backing (BB), and a layering technique (enamel over dentin composite) with the corresponding Vita Classical Shade tabs (VST).

Materials And Methods: Composite disk specimens enamel (N = 5) shades B1, B2, A1, and A2, and dentin (N = 1) shades A1, A2, and A3 were made. The color of the VST B1, B2, A1, and A2, enamel shade disks, and layering composites of the same brand and different brands were assessed using a colorimeter over a WB and BB.

Effect of heat on the flow of commercial composites.

Purpose: This study compared the flowability of various commercial dental composites to flowable composites in response to warming in a composite warmer.

Methods: 18 conventional resin composites and four flowable composites were tested at room temperature (23 degrees C), and the conventional composites were tested after pre-heating in a Calset unit (Addent) to 54 degrees C or 68 degrees C. Flowability was determined by placing uncured composite onto plastic sheets and loading for 180 seconds (4 kg load) while maintained at 36 degrees C (conditioning temperature [n=3]) to simulate placing room temperature composite into a tooth.

Who deserves the credit for discovering ether's use as a surgical anesthetic?

This paper discusses the controversy based around the discovery of inhalation anesthesia. Although many contributors claim to be the discoverer, deeper research into the events leading to anesthesia can award credit to a select few. It can be determined that anesthesia in animals was discovered by Paracelsus back in the fifteenth century and that the first person to demonstrate ether's use as a surgical anesthetic was Dr.

Effects of new formulas of bleaching gel and fluoride application on enamel microhardness: an in vitro study.

This in vitro study evaluated the new formulas of bleaching products and the effect of subsequent applications of fluoride on the hardness of enamel during and after tooth bleaching. The crowns of 60 extracted intact human molars were sectioned longitudinally; the buccal part was embedded in acrylic resin, the occlusal part was ground flat, exposing enamel and dentin, and then polished. Baseline Knoop microhardness (KHN) of enamel was determined.