Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in .

Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs.

Constructing well-dispersed active phase spontaneous redox for electrochemical nitrate reduction to ammonia.

In this study, a distinctive multiple core-shell structure of Co nanoparticles inserted into N-doped carbon dodecahedron@Co hydroxide (Co/NCD@Co(OH)) was synthesized a spontaneous redox reaction between metallic Co and NO, ultimately materializing the fine dispersion and exposure of the active sites. The electronic interaction existing between the Co/NCD core and the Co(OH) shell brings a synergistic effect, conspicuously lessens the overpotential, and reinforces the yield-rate and faradaic efficiency of NH for electrochemical nitrate-ammonia conversion. This study underlines the spontaneous redox between the catalysts and substrate, rendering it as a synthetic strategy for designing genuine and well-dispersed active sites.

Simultaneous Unbinned Differential Cross-Section Measurement of Twenty-Four Z+jets Kinematic Observables with the ATLAS Detector.

Z boson events at the Large Hadron Collider can be selected with high purity and are sensitive to a diverse range of QCD phenomena. As a result, these events are often used to probe the nature of the strong force, improve Monte Carlo event generators, and search for deviations from standard model predictions. All previous measurements of Z boson production characterize the event properties using a small number of observables and present the results as differential cross sections in predetermined bins.

Association Between Preterm Birth and Fulfillment of Desired Permanent Contraception.

Objectives: In cases of preterm delivery, the Medicaid sterilization policy mandates a signed consent form at least 72 h before surgery for permanent contraception, which is less than the 30 day minimum waiting period for term births. This study evaluated the association between preterm birth and fulfillment of planned permanent contraception.

Study Design: This was a secondary analysis of a multi-center retrospective cohort study of 3013 patients with a postpartum contraceptive plan of permanent contraception.

Effect of Feeding Mice Soluble Metals and Heavy Metal Contaminated Soil on Feces Metal Concentrations.

The effect of heavy metal availability and interaction in feed on feces heavy metal excretion in mice has rarely been investigated. In this work, feed containing a polluted soil (total Cd = 6.34, total Pb = 387 mg kg) amended with phosphate, bentonite and lime, or feed spiked with soluble Pb and Cd were fed to mice for 10 days.

The Impact of Physical Therapy Postprofessional Education Programs on Productivity in a Large Academic Medical Center.

Introduction: Emerging evidence shows positive impact of postprofessional physical therapy education (residency and fellowship) specific to participants; however, outcomes on organizational impact are largely unknown. The purpose of this project was to describe the impact residency and fellowship training has on financial metrics. A secondary purpose of this case study was to describe trends associated with higher productivity.

Enantiomer-Dependent Supramolecular Antibacterial Therapy for Drug-Resistant Bacterial Keratitis.

Bacteria have the potential to exhibit divergent stereochemical preferences for different levels of chiral structures, including from molecule, supramolecule, to nanomicroscale helical structure. Accordingly, the structure-activity relationship between chirality and bactericidal activity remains uncertain. In this study, we seek to understand the multivalent molecular chirality effect of chiral supramolecular polymers on antibacterial activity.

Long-range ordered high-entropy intermetallics enable stable and efficient hydrogen evolution in seawater.

Long-range ordered high-entropy intermetallics (HEIs) were synthesized a thermodynamically-driven atomic ordering strategy. The (FeCoNi)(RuPt) HEI achieves 200 mA cm at an overpotential of 56 mV and a remarkable low Tafel slope of 50.4 mV dec in alkaline seawater.

Towards the Stable Chelation of Radioantimony(V) for Targeted Auger Theranostics.

Antimony-119 (119Sb) is one of the most attractive Auger-electron emitters identified to date, but it remains practically unexplored for targeted radiotherapy because no chelators have been identified to stably bind this metalloid in vivo. In a departure from current studies focused on chelator development for Sb(III), we explore the chelation chemistry of Sb(V) using the tris-catecholate ligand TREN-CAM. Through a combination of radiolabeling, spectroscopic, solid-state, and computational studies, the radiochemistry and structural chemistry of TREN-CAM with 1XX/natSb(V) were established.

Molecular dynamics simulations of functionalized hBN nanopores in water: Ab initio force field and implications for water desalination.

Heteropolar two-dimensional materials, including hexagonal boron nitride (hBN), are promising candidates for seawater desalination and osmotic power harvesting, but previous simulation studies have considered bare, unterminated nanopores in molecular dynamics (MD) simulations. There is presently a lack of force fields to describe functionalized nanoporous hBN in aqueous media. To address this gap, we conduct density functional theory (DFT)-based ab initio MD simulations of hBN nanopores surrounded by water molecules.

Feasibility and safety of hyperthermic intra-peritoneal chemotherapy in patients with ovarian cancer and chronic kidney disease.

Objective: This study aimed to compare perioperative outcomes and progression-free and overall survival in patients with chronic kidney disease (CKD) versus those without after hyperthermic intra-peritoneal chemotherapy (HIPEC) for ovarian cancer.

Methods: This is a retrospective, single-institution cohort study of patients with ovarian cancer treated with HIPEC at the Cleveland Clinic from January 2009 to December 2022. All patients received HIPEC with cisplatin and renal protection with mannitol and furosemide.

PP2A Attenuates Thoracic Aneurysm and Dissection in Mouse Models of Marfan Syndrome.

Background: Recent studies show that hyperactivation of mTOR (mammalian target of rapamycin) signaling plays a causal role in the development of thoracic aortic aneurysm and dissection. Modulation of PP2A (protein phosphatase 2A) activity has been shown to be of significant therapeutic value. In light of the effects that PP2A can exert on the mTOR pathway, we hypothesized that PP2A activation by small-molecule activators of PP2A could mitigate AA progression in Marfan syndrome (MFS).

A protocol for the investigation of the intramolecular vibrational energy redistribution problem: the isomerization of nitrous acid as a case of study.

The conformational isomerization of nitrous acid (HONO) promoted by excitation of the or stretching normal coordinates is the first observed case of an infrared-induced photochemical reaction. The energy captured by the excited normal modes is redistributed into a highly excited vibrational level of the torsion normal coordinate, which is the isomerization reaction coordinate. Herein, we present simple numerical methods to qualitatively investigate the coupling between the normal coordinates and the possible gateways for vibrational energy redistribution leading to the isomerization process.

Resilience Profiles among Grandparents Raising Grandchildren in the United States: White and Black Differences.

Background And Objectives: Grandparents raising grandchildren face many challenges and stress regardless of race and ethnicity; however, they are generally resilient. The present study aims to classify resilience profiles of these grandfamilies using a person-centered approach and examine the association of race and ethnicity with these profiles.

Research Design And Methods: The present study analyzed cross-sectional survey data collected from grandparents raising grandchildren in the United States (N = 287).

Quantifying the ability of the CFH group as a hydrogen bond donor.

The CFH group can act as a hydrogen bond donor, serving as a potential surrogate for OH or SH groups but with a weaker hydrogen bond donation ability. Here, we describe a series of CFH group-containing moieties that facilitate hydrogen bond interactions. We survey hydrogen bond donation ability using several established methods, including H NMR-based hydrogen bond acidity determination, UV-vis spectroscopy titration with Reichardt's dye, and H NMR titration using tri--butylphosphine oxide as a hydrogen bond acceptor.

The Clinical Course of Portopulmonary Hypertension and Outcomes With Endothelin Receptor Antagonist Treatment: Observational Study of Data From the US Organ Procurement and Transplantation Network.

Background: Portopulmonary hypertension (PoPH) occurs in patients with advanced liver disease and can be a contraindication to liver transplant (LT). Improvement of hemodynamic parameters with pulmonary arterial hypertension (PAH) therapies (including endothelin receptor antagonists [ERAs]) may help some patients to become eligible for LT.

Methods: We conducted a retrospective secondary data analysis to describe the clinical course and management of PoPH in patients on a US registry LT waitlist and outcomes in patients receiving an ERA.

Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats.

Objectives: While ketone bodies are not the main heart fuel, exercise may increase their uptake. Objectives: This study aimed to investigate the effect of 6-week endurance training and Pyruvate dehydrogenase kinase 4 )PDK4( inhibition on ketone bodies metabolism in the heart of diabetic rats with emphasis on the role of Peroxisome proliferator-activated receptor-gamma coactivator PGC-1alpha (PGC-1α).

Materials And Methods: Sixty male Wistar rats were divided into eight groups: healthy control group (CONT), endurance training group (TRA), diabetic group (DM), DM + EX group, Dichloroacetate (DCA) group, DM + DCA group, TRA + DCA group, and DM + TRA + DCA group.

Removal of Cr(VI) from aqueous solutions by activated carbon and its composite with PWO: A spectroscopic study to reveal adsorption mechanism.

Molecular scale information is needed to understand ions coordination to mineral surfaces and consequently to accelerate the design of improved adsorbents. The present work reports on the use of two-dimensional correlation Fourier Transform infra-red spectroscopy (2D-COS-FTIR) and hetero 2D-COS-FTIR- X-ray diffraction (XRD) to probe the mechanism of Cr(VI) removal from aqueous solutions by activated carbon (AC) and its composite with PWO (AC-composite). The adsorption data at an initial Cr(VI) concentration of 320 mg L (320 ppm) revealed maximum adsorption capacities of 65 mg g for AC and 73 mg g for AC-composite, corresponding to removal percentages of 83 % and 94 %, respectively.

A comparative X-ray diffraction analysis of Srsubstituted hydroxyapatite from sand lobster shell waste using various methods.

This study aims to investigate the crystallographic properties of hydroxyapatite (HAp) and strontium-substituted hydroxyapatite (SrHAp) obtained from sand lobster shells (SLS) using various analytical methods. HAp and SrHAp were synthesized by the hydrothermal method using sand lobster () shell waste as a calcium precursor. SLS were calcined at 0 °C, 600 °C, 800 °C, and 1000 °C and characterized by X-ray diffraction (XRD).

Development of functionalized biochar composites for enhanced boron adsorption from aqueous solutions.

This study focuses on developing biochar-based adsorbents with high adsorption capacity and rapid adsorption rates for removing boron from aqueous solutions. Hydroxy-enriched biochar composites (BC (carboxylated biochar), BC-PDA (polydopamine loaded biochar), MBC-PDA (polydopamine loaded magnetic biochar), BC-AlOOH (AlOOH loaded biochar), and BC-ZnCl (biochar modified by ZnCl)) were synthesized specifically for boron adsorption to utilize the superior adsorption capacity of biochar. All adsorbents were synthesized using straightforward experimental techniques from date palm cellulosic fibers as promising lignocellulose feedstock and subjected to various characterization methods.

Implementation of a Cost and Variation Reduction Program for Hemostasis Products at a Large Academic Medical Center: A Multi-Stakeholder Perspective.

Goal: Cost-saving initiatives targeting surgical supplies management have become increasingly common to address rising healthcare costs. However, few studies provide details on hospital stakeholder experiences or learnings from implementing such initiatives. Thus, we sought to evaluate hospital stakeholder satisfaction with conversion to a sole supplier for hemostasis products, in addition to economic and clinical impacts, to help develop best practices for implementation.

Effect of Manipulation Methods and Storage Environments on the Microstructural, Chemical, and Mechanical Properties of Calcium-Enriched Mixture Cement.

This study aimed to evaluate the impact of different manipulation methods and storage environments on the microstructural, chemical, and mechanical properties of calcium-enriched mixture (CEM) cement. Four sample groups were examined, including nondried (ND-I) and dried (D-I) groups placed directly in an incubator, dried samples stored in phosphate-buffered saline (PBS) (D-P), and dried samples stored in distilled water (D-W). Various analyses, including Vickers microhardness, compressive strength, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS) were conducted after incubating the samples for 7 days.

Consumer Products Nerve Injuries Among Patients Products Presenting to United States Emergency Departments Between 2012 and 2021: A Nationwide Cohort.

Introduction: Nerve injuries and resultant pain are common causes of emergency department (ED) visits in the United States. Injuries often occur either due to activity (ie sports related injury) or due to consumer products such as stairs or bedframes. We investigated the incidence of consumer product-related nerve injuries (CPNIs) in patients who presented to the ED in the United States.