COMPLEX ANALYSIS OF THE ROLE OF CYTOKINE GENE POLYMORPHISMS AS PROGNOSTIC FACTOR OF THE RISK OF PLASMA CELL MYELOMA IN PERSONS SUFFERED AFTER THE CHORNOBYL NPP ACCIDENT.

Objective: to provide a comparative characterization of the prevalence of polymorphic variants of cytokine genes in plasma cell myeloma (PCM) patients suffered after the Chornobyl disaster and patients who were in contact with ionizing radiation within the natural radiation background, based on comparison with population controls to determine their contribution as genetic markers of disease risk.

Materials And Methods: Molecular genetic studies of polymorphism of cytokine genes (TNF-α, TGF-β1, IL-6, IL-10, IFN-γ) and complex frequency analysis of occurrence in three-, four-, and five-locus combinations of their allelic variants as prognostic markers of the risks of plasma cell myeloma was carried out in 102 patients - 56 victims of the Chornobyl nuclear power plant accident and 46 patients irradiated within the limits of the natural radiation background, in comparison with the control group (364 practically healthy people, residents of the Central geno-geographical region of Ukraine).

Results: The same probable increase in the prevalence of the TGF-β genotype codon10 T/T of the TGF-β1 gene was established in the groups of patients irradiated after the Chornobyl NPP accident and non-irradiated patients.

Millimeter waves alter DNA secondary structures and modulate the transcriptome in human fibroblasts.

As millimetre wave (MMW) frequencies of the electromagnetic spectrum are increasingly adopted in modern technologies such as mobile communications and networking, characterising the biological effects is critical in determining safe exposure levels. We study the exposure of primary human dermal fibroblasts to MMWs, finding MMWs trigger genomic and transcriptomic alterations. In particular, repeated 60 GHz, 2.

Assessment of gold nanoparticle effect on prostate cancer LNCaP cells.

Unlabelled: In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells.

Aims: This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth.

The effect of intermittent hypoxic training on lung and heart tissues of healthy rats.

Introduction: Recently, particular attention has been focused on the problem of the beneficial influence of intermittent hypoxia (IH) on the human organism. However, knowledge regarding the negative effects of intermittent hypoxic training (IHT) on cellular adaptive mechanisms remains limited. The aim of the present study was to investigate: 1) lung and heart ultrastructural changes under IHT; and 2) the adequateness of morphological and morphometric methods to determine the constructive and destructive displays of hypoxia.

Cytochrome C as an amplifier of ROS release in mitochondria.

The influence of exogenous cytochrome c on reactive oxygen species (ROS) formation and its dependence on mitochondrial permeability transition pore (MPTP) opening is studied in rat liver mitochondria. Fluorescent probe dichlorofluorescein (DCF) was used. It was shown that MPTP activation by increasing concentrations of Ca2+ in the medium results in the increase in mitochondrial ROS production and oxygen consumption, but the decrease in matrix calcium retention, dependent on the amount of added Ca2+.

[Electrophysiological study of tissue hydration in typical non-specific pulmonary pathology].

Computer occlusive electrical mioplethysmography and common electrical impedance meter method were used for the study of the tissue hydration in patients with bronchial asthma (BA) exposed to intermittent normobaric hypoxia (INH). Hyperhydration of intracellular and extracellular compartments in patients with BA was shown. INH significantly decreased tissue hyperhydration.

[Effect of L-arginine on the endothelium functional activity in experimental diabetes mellitus].

The experimental data about the shift of L-arginine metabolism in the direction of activization of non-oxidizing (arginase) way under experimental diabetes mellitus are presented. This shift was proved by an increase of the ratio of arginase and NO-synthase activity and an increase of the ratio of their metabolites. The decrease of the free L-arginin contents in tissues of animals with experimental diabetes mellitus is shown.

[Cardiac reflexes under conditions of heart injury].

Analysis of the previous experimental literary data and own authors data about the pattern of cardiogenic reflectory effects during rise and development of the local injury is represented in this review. The role of double afferent innervation of the heart is discussed and author suggests its mayor functional role under some kind of heart diseases.

Involvement of nitric oxide in regulation of the medullary respiratory rhythm in neonatal rats.

The NADPH-diaphorase (as a neuronal NO-synthase) reactivity in the medullary structures of the respiratory rhythm (RR) generator and the role of NO in the regulation of respiratory activity in the phrenic nerve of artificially superfused semi-isolated medulla-spinal cord preparations were investigated in newborn rats. NADPH-diaphorase positive neurons were found in all nuclei of both dorsal and ventral respiratory groups of neurons. The maximal density of stained cells was present within the rostral part of the ventrolateral medulla (VLM), in the region of the lateral paragigantocellular reticular nucleus.

[The dynamics of the formation of the properties of the main nervous processes in children 4-7 years old].

Dynamics of formation of characteristics of the main nervous processes (functional mobility and power), their connection with the results of mental activity of children under school age and the level of their preschool readiness have been studied. It has been shown that neurodynamic processes develop progressively within a period from four to seven years of age. Mental ability of children under school age is substantially determined by characteristics of the main nervous processes.

[Reactive hyperemia in hypertension].

This study was designed to investigate whether endothelium-dependent reactive hyperemia (RH) is altered in patients with hypertensive disease (HD). In 35 mildly (HDI) patients, 38 hardly (HDII) patients, and 41 age-matched healthy subjects being as a control (C), forearm blood flow (FBF) was measured by means of impedance plethysmography before and after 30-second occlusion. Resting FBF was comparable in all groups while the maximal increase in FBF during RH was reduced in HD versus that in healthy.