22 results match your criteria: "Norwegian National Hospital[Affiliation]"
ScientificWorldJournal
June 2012
Vilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, Norway.
Several studies have implicated the aquaporins (aqp) 1, 4, and 9 in the pathogenesis of malignant brain tumours, suggesting that they contribute to motility, invasiveness, and oedema formation and facilitate metabolism in tumour cells under hypoxic conditions. We have studied the expression of aqp1, 4, and 9 in biopsies from glioblastomas, isolated tumour stem cells grown in a tumoursphere assay and analyzed the progenitor and differentiated cells from these cultures. We have compared these to the situation in normal rat brain, its stem cells, and differentiated cells derived thereof.
View Article and Find Full Text PDFNeuro Oncol
December 2010
Institute for Surgical Research, Norwegian National Hospital, Oslo, Norway.
Traditional in vitro culturing of tumor cells has been shown to induce changes so that cultures no longer represent the tumor of origin. Serum-free culturing conditions are used in a variety of cancers to propagate stem-like cells in vitro. Limited reports, however, exist on the effects of such propagation.
View Article and Find Full Text PDFInt J Oncol
May 2004
Institute of Pathology, Norwegian National Hospital, N-0027 Oslo, Norway.
5-Fluorouracil (5-FU) is the chemotherapeutic drug of choice for the treatment of metastatic colorectal cancer, but resistance to 5-FU remains a major obstacle to successful therapy. We generated 5-FU-resistant derivatives of the HCT116 human colon cancer cell line by serial passage of these cells in the presence of increasing 5-FU concentrations in an attempt to elucidate the biological mechanisms involved in resistance to 5-FU. Two resultant resistant derivatives, HCT116 ResB and ResD, were characterized for resistance phenotypes, genotypes, and gene expression using cells maintained long-term in 5-FU-free media.
View Article and Find Full Text PDFTransplant Proc
March 2003
Norwegian National Hospital, University of Oslo, Oslo, Norway.
Int J Colorectal Dis
February 2001
Institute of Pathology, Norwegian National Hospital, 0027 Oslo, Norway.
Colorectal carcinomas are characterized by frequent recurrent gains and losses of chromosomal material, especially gains of chromosome arms 20q and 13q, and losses of chromosome arms 18q and 4q. These may be important in the development and progression of colorectal carcinomas. Chromosomal aberrations detected by comparative genomic hybridization in 67 sporadic colorectal carcinomas were examined for their possible associations with patient survival.
View Article and Find Full Text PDFJ Photochem Photobiol B
November 2000
Radiation Medicine Department, Norwegian Radiation Protection Authority and Institutes of Radiology and Pathology, Norwegian National Hospital, University of Oslo.
Cells from the mouse lymphoma cell line L5178Y-R were exposed to blue light from phototherapy lamps in the presence of solutions of 160 microM bilirubin supplemented with serum albumin. HPLC analysis showed that the bilirubin solution was photooxidised as a function of increasing light dose. The cells were stained with trypan blue to score necrosis, and apoptosis was assayed by the terminal deoxynucleotide transferase assay (TdT) or by studying the nuclear structure in cells stained with propidium iodide.
View Article and Find Full Text PDFComparative genomic hybridization (CGH) is used to detect amplified and/or deleted chromosomal regions in tumours by mapping their locations on normal metaphase chromosomes. Forty-five sporadic colorectal carcinomas were screened for chromosomal aberrations using direct CGH. The median number of chromosomal aberrations per tumour was 7.
View Article and Find Full Text PDFMol Pathol
October 1998
Norwegian National Hospital, Institute for Pathology/University of Oslo, Norway.
Aims: Spontaneous apoptosis and expression of the apoptotic regulatory proteins Bax, Bcl-x, and Bcl-2 were investigated in 50 colorectal carcinomas. The p53 genotypes/phenotypes and BAX genotypes were also determined, and possible associations of these with apoptosis and/or with expression of the different apoptotic regulatory proteins were studied.
Methods: Terminal deoxynucleotidyl transferase (TdT) mediated dUTP labelling of DNA fragments was used to detect apoptotic tumour cells in sections and peroxidase immunohistochemistry was used to assess protein expression.
Mol Pathol
August 1997
Institute for Pathology, Norwegian National Hospital, Oslo, Norway.
Aims: To investigate the expression of nucleolar protein NO38, to determine the numbers of nucleoli per cell, and to examine the relations of these nucleolar parameters to tumour DNA index, total cellular DNA content, S phase fraction, and Ki67 labelling index.
Methods: 36 colorectal tumours and 14 normal mucosas were studied. An anti-NO38 monoclonal antibody, 31A12, and flow cytometric analysis were used to detect expression of NO38 by means of a biotin-streptavidin-FITC (fluorescein isothiocyanate) staining method.
J Hosp Infect
December 1996
Department of Plastic Surgery, Norwegian National Hospital, University Hospital of Oslo, Norway.
During two separate periods a total of 654 patients were included in a clinical study relating preoperative bacterial colonization to occurrence of postoperative wound infection in plastic surgery. During the second period one half of the patients were randomized to receive prophylactic azithromycin. Bacteriological samples were collected from the nasal vestibulum during both periods, and additionally from the surgical field during the second period.
View Article and Find Full Text PDFPlast Reconstr Surg
November 1995
Department of Plastic Surgery, Norwegian National Hospital, Oslo, Norway.
Over a 9-month period from September of 1991 to May of 1992, 339 patients were included in a randomized, double-blind, placebo-controlled study using azithromycin as the prophylactic agent to determine whether it effects a clinically meaningful reduction in postoperative surgical infections in plastic surgery. Azithromycin was given as prophylaxis in 171 patients and placebo in 168 patients. The study medication was a single oral dose taken at 8 P.
View Article and Find Full Text PDFPlast Reconstr Surg
September 1995
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
In a postoperative wound infection study in plastic surgery, 315 patients were randomized to either outpatient wound control after 30 days (group I) or self-control by questionnaire (group II). We present a new definition of wound infection based on physiologic wound healing. The surveillance of postoperative wound infection showed follow-up rates of 95 and 68 percent and infection rates of 16.
View Article and Find Full Text PDFBr J Cancer
August 1995
Institute of Pathology, Norwegian National Hospital, Oslo.
Overexpression of the MDR1 product, P-glycoprotein (Pgp), has been shown to be one of the mechanisms underlying the development of multidrug resistance (MDR). Recently, one mutant p53 has been shown to stimulate the MDR1 gene promoter in vitro, whereas wild-type p53 repressed this activity. We measured Pgp and p53 expression by immunoblotting in 34 colorectal tumours, and performed mutation analyses on the p53-positive tumours to confirm the presence of mutant p53 protein.
View Article and Find Full Text PDFScand J Plast Reconstr Surg Hand Surg
June 1995
Department of Plastic Surgery, Norwegian National Hospital, University Hospital of Oslo.
The amount of blood lost during liposuction with the "dry" or classic "wet" techniques has been a cause for concern. In the present study 26 consecutive patients who underwent syringe-assisted liposuction with the "superwet" or "tumescent" technique had their blood loss recorded prospectively. The mean (SD) volume aspirated was 2448 (1368) ml and the mean (SD) drop in haemoglobin concentration was 11 (7) g/l.
View Article and Find Full Text PDFEur J Surg
May 1995
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
Objective: To investigate the effectiveness of liposuction as treatment in troublesome colostomies and urinary stomas in selected patients.
Design: Open clinical study.
Setting: University hospital, Norway.
Scand J Plast Reconstr Surg Hand Surg
March 1995
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
During a five year period, 71 patients (age range 24-72 years) underwent syringe-assisted liposuction of the neck as the only operation for facial rejuvenation. The patients were seen after one week and three months, and 66 patients were re-examined 5-58 months (mean 31) postoperatively. There were no complications except for slight skin laxity (n = 10), transient hypoaesthesia, and temporary subcutaneous scarring (nodules).
View Article and Find Full Text PDFScand J Plast Reconstr Surg Hand Surg
June 1994
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
Since liposuction became part of our surgical regimen in 1988, we have operated on 67 patients for gynaecomastia during the five year period 1988-1992. Sixty two of the patients were seen at an extra follow up 4-59 months (means 29 months) postoperatively. Compared to studies that did not include liposuction as part of the operation, we found a lower incidence of postoperative complications and a higher degree of patient satisfaction.
View Article and Find Full Text PDFPlast Reconstr Surg
May 1994
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
Liposuction, like many other plastic surgical procedures, is often performed under local anesthesia. Drug toxicity is the most serious complication and the factor that limits the use of this form of anesthesia. Toxic effects are related to the peak concentration in plasma and depend on the type of local anesthetic, the drug concentration, total dose, site of injection, injection speed, and whether vasoconstrictors are used or not.
View Article and Find Full Text PDFScand J Plast Reconstr Surg Hand Surg
March 1994
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
A double blind, randomised controlled trial was performed to compare the amount of pain experienced after subcutaneous infiltration of lignocaine with adrenaline, and the same anaesthetic buffered to pH 7.1-7.3, in patients undergoing liposuction (n = 8), blepharoplasty (n = 7), mammaplasty (n = 6), and in volunteers (n = 10).
View Article and Find Full Text PDFScand J Plast Reconstr Surg Hand Surg
December 1993
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
J Intern Med
November 1993
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
Objectives: To investigate the effectiveness of liposuction as treatment for lipohypertrophy in insulin-treated diabetic patients.
Design: Open clinical study.
Setting: Norwegian National Hospital, Oslo.
Scand J Plast Reconstr Surg Hand Surg
January 1993
Department of Plastic Surgery, Norwegian National Hospital, Oslo.
A retrospective study was made of 87 patients operated on between 1984 and 1990 for asymmetrical breasts. Thirty-two patients had 36 implants (13 also had breast reduction or mastopexy of the contralateral breast). Capsular contraction, Baker grade II-IV, was found in a third of the implants.
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