Differential Transcriptomic Profile of LF-89 and EM-90 During an Spatial Separation Co-Culture in Atlantic Salmon.

Salmonid rickettsial septicemia (SRS) is a critical sanitary problem in the Chilean aquaculture industry since it induces the highest mortality rate in salmonids among all infectious diseases. , a facultative intracellular bacterium, is the biological agent of SRS. In Chile, two genogroups of , designated as LF-89 and EM-90, have been identified.

Evaluation of the Wear of Ni 200 Alloy After Long-Term Carbon Capture in Molten Salts Process.

Reducing CO emissions is one of the major challenges facing the modern world. The overall goal is to limit global warming and prevent catastrophic climate change. One of the many methods for reducing carbon dioxide emissions involves capturing, utilizing, and storing it at the source.

Durability of Wood-Cement Composites with Modified Composition by Limestone and Stabilised Spruce Chips.

Limestone (LS) and stabilised secondary spruce chips (SCs) utilisation in wood-cement composites is still an unexplored area. Therefore, the main objective of the research presented here is the assessment of the long-term behaviour of cement-bonded particleboards (CBPs) modified by LS and SCs. Cement (CE) was replaced by 10% of LS, and spruce chips by 7% of SCs.

DFT Study of the Stability and Electronic Properties of Ni-Doped Defected (6,0) and (8,0) Single-Walled Carbon Nanotubes.

The interaction of Ni with (6,0) and (8,0) zigzag carbon nanotube exterior surfaces containing two vacancies was studied using density functional theory (DFT). A two-vacancy defect was analysed in order to anchor Ni, and the pristine nanotube was also considered as a reference for each chirality. The adsorbed Ni stability and the nanotube's geometry and electronic structure were analysed before and after the adsorption.

Novel Numerical Method for Studying Water Freezing on Surfaces Texturized by Laser.

Within this study, a methodology for the numerical simulation of droplet freezing, including a micrometer texturized pattern, was developed. The finite volume method was then applied to simulate the behavior of water droplets. The procedure was divided into two processes: stabilization and freezing.

Phase Evolution of Li-Rich Layered Li-Mn-Ni-(Al)-O Cathode Materials upon Heat Treatments in Air.

The phase evolution of Li-rich Li-Mn-Ni-(Al)-O cathode materials upon heat treatments in the air at 900 °C was studied by X-ray and neutron powder diffraction. In addition, the structures of LiMnAl NiO, x = 0.0, 0.

Cytotoxic Natural Products from (Thunb. ex L.) D.Don.

is a commercially important tree native to Japan. The tree belongs to the ancient genus and has found important uses as a medicinal plant, as well as a main source of timber in Japan. In recent years, there has been an increased interest in discovering extended uses of as a source of novel bioactive natural products with potential applications as lead compounds for active principles of future drugs.

Precise and Accurate DNA-3'/5-Ends Polishing with Phage vb_Tt72 DNA Polymerase.

Tt72 DNA polymerase is a newly characterized PolA-type thermostable enzyme derived from the phage vB_Tt72. The enzyme demonstrates strong 3'→5' exonucleolytic proofreading activity, even in the presence of 1 mM dNTPs. In this study, we examined how the exonucleolytic activity of Tt72 DNA polymerase affects the fidelity of DNA synthesis.

In Vivo Induction of Leukemia-Specific Adaptive and Innate Immune Cells by Treatment of AML-Diseased Rats and Therapy-Refractory AML Patients with Blast Modulating Response Modifiers.

There is a high medical need to develop new strategies for the treatment of patients with acute myeloid leukemia (AML) refractory to conventional therapy. In vitro, the combinations of the blast-modulatory response modifiers GM-CSF + Prostaglandin E1, (summarized as Kit M) have been shown to convert myeloid leukemic blasts into antigen-presenting dendritic cells of leukemic origin (DC) that were able to (re-)activate the innate and adaptive immune system, direct it specifically against leukemic blasts, and induce memory cells. This study aimed to investigate the immune modulatory capacity and antileukemic efficacy of Kit M in vivo.

Endoscopic Ultrasound-Guided Pancreatic Tissue Sampling: Lesion Assessment, Needles, and Techniques.

Endoscopic ultrasound (EUS)-guided tissue sampling includes the techniques of fine needle aspiration (FNA) and fine needle biopsy (FNB), and both procedures have revolutionized specimen collection from the gastrointestinal tract, especially from remote/inaccessible organs. EUS-FNB has replaced FNA as the procedure of choice for tissue acquisition in solid pancreatic lesions (SPLs) across various society guidelines. FNB specimens provide a larger histological tissue core (preserving tissue architecture) with fewer needle passes, and this is extremely relevant in today's era of precision and personalized molecular medicine.

arterioscope.sim: Enabling Simulations of Blood Flow and Its Impact on Bioimpedance Signals.

Objectives: Early detection of cardiovascular diseases and their pre-existing conditions, arteriosclerosis and atherosclerosis, is crucial to increasing a patient's chance of survival. While imaging technologies and invasive procedures provide a reliable diagnosis, they carry high costs and risks for patients. This study aims to explore impedance plethysmography (IPG) as a non-invasive and affordable alternative for diagnosis.

Neurocognitive Profile and Associated Factors Among Children Affected by Sickle Cell Disease in Kinshasa, Democratic Republic of Congo: A Cross-Sectional Study.

Background/objectives: Understanding the neurocognitive profile of children with sickle cell disease in the Democratic Republic of Congo is essential, as this condition can significantly affect their development. Our study aims to assess these children's neurocognitive and developmental profiles and identify related factors.

Methods: We conducted a descriptive cross-sectional study involving 287 children, aged 0 to 68 months, using the Mullen Scales of Early Learning and the Gensini Gavito Scale.

Making a Difference from Day One: The Urgent Need for Universal Neonatal Hearing Screening.

Neonatal hearing screening (NHS) is a critical public health measure for early identification of hearing loss, ensuring timely access to interventions that can dramatically improve a child's language development, cognitive abilities, and social inclusion. Beyond clinical benefits, NHS provides long-term advantages in education and quality of life. Given that congenital hearing loss affects approximately 1-2 in every 1000 newborns worldwide, the case for universal screening is clear.

Microbiota and Cytokine Modulation: Innovations in Enhancing Anticancer Immunity and Personalized Cancer Therapies.

The gut microbiota plays a crucial role in modulating anticancer immunity, significantly impacting the effectiveness of various cancer therapies, including immunotherapy, chemotherapy, and radiotherapy. Its impact on the development of cancer is complex; certain bacteria, like and , can stimulate the growth of tumors by causing immunological evasion and inflammation, while advantageous strains, like , have the ability to suppress tumors by modifying immune responses. Cytokine activity and immune system regulation are intimately related.

Scoping Review of Climate Change Adaptation Interventions for Health: Implications for Policy and Practice.

Climate change is among the greatest threats to health in the 21st century, requiring the urgent scaling-up of adaptation interventions. We aim to summarise adaptation interventions that were funded by the Belmont Forum and the European Union, the largest global funders of climate change and health research. A systematic search was conducted (updated February 2023) to identify articles on adaptation interventions for health within this funding network.

Targeted Genetic Education in Dentistry in the Era of Genomics.

The growing body of knowledge on the human genome and its variants points towards the significance of genetic factors in oral health and disease. Since the dental curricula have historically prioritized clinically oriented subjects, this focus has resulted in insufficient coverage of genetics. To leverage this knowledge in patient care, dental education must equip students with an understanding of the principles of genetics.

Interleukin-27 and Autoimmune Disorders: A Compressive Review of Immunological Functions.

Autoimmune disorders (ADs) pose significant health and economic burdens globally, characterized by the body's immune system mistakenly attacking its own tissues. While the precise mechanisms driving their development remain elusive, a combination of genetic predisposition(s) and environmental triggers is implicated. Interleukin-27 (IL-27), among numerous cytokines involved, has emerged as a key regulator, exhibiting dual roles in immune modulation.

Circulating Glutathione Peroxidase-3 in Elderly-Association with Renal Function, Cardiovascular Mortality, and Impact of Selenium and Coenzyme Q Supplementation.

Low-selenium status was associated with impaired renal function, which improved after selenium and coenzyme Q supplementation in an RCT. Here, we evaluated serum glutathione peroxidase-3 (GPx3) and its relation to serum selenium, selenoprotein P (SELENOP), renal function, mortality, and the impact of supplementation, which are all important, especially in elderly individuals. In total, 383 study participants (197 receiving selenium yeast and coenzyme Q and 186 on a placebo) were evaluated.

Accelerated Sarcopenia Phenotype in the DJ-1/-Knockout Zebrafish.

Age-dependent loss of muscle mass and function is associated with oxidative stress. DJ-1/ acts as an antioxidant through multiple signalling pathways. DJ-1-knockout zebrafish show a decline in swimming performance and loss of weight gain between 6 and 9 months of age.

Promising Probiotic Candidates for Sustainable Aquaculture: An Updated Review.

With the intensification of aquaculture to meet the rising demands of fish and shellfish, disease outbreaks during the larval and adult stages are a major challenge faced by aqua culturists. As the prophylactic use of vaccines and antibiotics has several limitations, research is now focused on sustainable alternatives to vaccines and antibiotics, e.g.

A novel super-resolution STED microscopy analysis approach to observe spatial MCU and MICU1 distribution dynamics in cells.

The uptake of Ca by mitochondria is an important and tightly controlled process in various tissues. Even small changes in the key proteins involved in this process can lead to significant cellular dysfunction and, ultimately, cell death. In this study, we used stimulated emission depletion (STED) microscopy and developed an unbiased approach to monitor the sub-mitochondrial distribution and dynamics of the mitochondrial calcium uniporter (MCU) and mitochondrial calcium uptake 1 (MICU1) under resting and stimulated conditions.

Dietary profiles of wild carnivores and Blastocystis occurrence: The case of the endangered Iberian lynx (Lynx pardinus) and systematic review.

Recent molecular and metagenomic studies have revealed that the obligate anaerobic protist Blastocystis is found more prevalently and with higher subtype diversities in herbivore species than in carnivore species. However, information on wild carnivore species is scarce. Here, we investigated the presence of Blastocystis by molecular methods in fecal DNA samples of free-ranging and captive Iberian lynxes from Spain (n = 243) and Portugal (n = 30).

Risk reducing mastectomy in Norwegian BRCA1/2 carriers.

Background: Risk reducing mastectomy (RRM) is an option for women with pathogenic germline variants in BRCA1 or BRCA2 (BRCA1/2). This study investigates and compares RRM-uptake among Norwegian BRCA1/2 carriers from 2008 to 2021, temporal trends, and incidence of breast cancer (BC) after surgery.

Methods: BRCA1/2 carriers without prior breast or ovarian cancer, tested at Oslo University Hospital between January 1st 2008 and December 31st 2021 were included in the study.

Mitochondrial dysfunction and lipid alterations in primary sclerosing cholangitis.

Objectives: Indications of mitochondrial dysfunction are commonly seen in liver diseases, but data are scarce in primary sclerosing cholangitis (PSC). Analyzing circulating and liver-resident molecules indirectly reflecting mitochondrial dysfunction, we aimed to comprehensively characterize this deficit in PSC, and whether this was PSC specific or associated with cholestasis.

Materials And Methods: We retrospectively included plasma from 191 non-transplant patients with large-duct PSC and 100 healthy controls and explanted liver tissue extracts from 24 PSC patients and 18 non-cholestatic liver disease controls.

Wooden breast myopathy is characterized by satellite cell dysfunction and syndecan-4 shedding.

Introduction: Skeletal muscle satellite cells (MuSCs or stem cells) play a crucial role in muscle development, maintenance, and regeneration, supporting both hypertrophy and regenerative myogenesis. Syndecans (SDCs) act as communication bridges within the muscle microenvironment, regulating interactions with extracellular matrix components and contributing significantly to tissue repair and inflammation. Specifically, syndecan-4 (SDC4) is involved in muscle regeneration at multiple stages.