Plasma transport of human growth hormone in vivo.

We have previously shown that a substantial part of human GH is complexed with GH-binding proteins (BPs) when GH is incubated with plasma in vitro. The proportion of GH bound in vivo, however, is unknown and may differ because of factors that cannot be assessed in vitro, such as binding to tissue receptors, distribution of GH outside the vascular compartment, and fluctuating GH and possibly BP levels. Accordingly, we studied the plasma transport characteristics of GH in vivo in six normal men.

Absorption of biliary calcium from the canine gallbladder: protection against the formation of calcium-containing gallstones.

Calcium salts are the major components of pigment gallstones. The calcium species in bile that is critical for Ca++ precipitation is free ionized calcium, [Ca++]. Factors that regulate biliary [Ca++] in bile are therefore of great importance in the pathogenesis of pigment gallstones.