[Expression of xenogentic epidermal growth factor receptor ectodomain and its antitumor effect].

Aim: To construct the EGFR protein vaccine and evaluate its antitumor effect.

Methods: The gene of chicken EGFR ectodomain was amplified by PCR, inserted into the pGEX-4T-2 vetor, and then exporessed in E.coli BL21 with glutathione S-transferase in a fusion protein form.

[Biogas yield and its relations with the duration and temperature of mixed anaerobic fermentation of livestock dungs and wheat straw].

To approach the relationships between the biogas yield ot mixed anaerobic termentation of livestock dungs and crop straw and the fermentation duration and temperature is the key of selecting fermentation materials for rural household biogas, determining optimal fermentation temperature, and improving the reuse efficiency of agricultural residues. In this paper, a batch of experiments under the condition of 8% mass fraction of total solid were conducted in a self-manufactured anaerobic fermentation reactor, with pig dung, cattle dung, and wheat straw as fermentation materials, and the substrate of constant temperature fermentation pool as inoculation substance. The biogas yield, fermentation duration, and optimal temperature were determined.

[Spatial variability of slope land soil solute transport parameters].

In this paper, the transport parameters of chlorine in a slope land soil were determined in situ by fitting breaking-though curve, and the spatial variability of the parameters in a 41 m x 5 m runoff plot was analyzed by using traditional and geo-statistics. The results showed that the average pore-water velocity increased gradually from the top to the bottom of the slope, and the diffusive-dispersive coefficient (D) had no remarkable variation within 20m away from the top but gradually increased with the distance beyond 20m. The dispersivity (alpha) had the same characteristics as D.

[Effects of osmotic stress on chlorophyll fluorescence parameters of wheat seedling].

By using chlorophyll fluorescence kinetics technology, this paper determined the chlorophyll fluorescence parameters Fv/Fm, phiPSII, qP, qNP, ETR of winter wheat seedling under simulated osmotic stress. The results showed that with the increase of osmotic stress, Fv/Fm and Fv/Fo had the trend of decrease-increase-decrease, indicating that photoinhibition did not happen at the first stage of osmotic stress, but the followed increase of Fv/Fm resulted in the photoinhibition and the decrease of phiPSII and ETR. During the course of osmotic stress, qP and qNP decreased firstly and then increased, which was helpful to enhance the open ratio of PSII reaction center, and made more photosynthetic energy use the photosynthetic electron transport to enhance the electron transport capacity.

[Mapping of apple Co gene using SSR markers].

Columnar apple is an important genetic resource for tree form breeding of apple. In this study, 106 individuals of the F1 population derived from 'Spur Fuji' (coco)x 'Telamon'(Coco) were used as plant materials for screening SSR markers linked to gene. By bulked segregating analysis (BSA), eight SSR markers from the tenth linkage group of apple genome were tested.

Effects of near-surface hydraulic gradients on nitrate and phosphorus losses in surface runoff.

Phosphorous (P) and nitrogen (N) in runoff from agricultural fields are key components of nonpoint-source pollution and can accelerate eutrophication of surface waters. A laboratory study was designed to evaluate effects of near-surface hydraulic gradients on P and N losses in surface runoff from soil pans at 5% slope under simulated rainfall. Experimental treatments included three rates of fertilizer input (control [no fertilizer input], low [40 kg P ha(-1), 100 kg N ha(-1)], and high [80 kg P ha(-1), 200 kg N ha(-1)]) and four near-surface hydraulic gradients (free drainage [FD], saturation [Sa], artesian seepage without rain [Sp], and artesian seepage with rain [Sp + R]).

Screening of a RAPD marker tightly linked to Co gene in apple and the SCAR marker conversion.

A DNA marker linked to the columnar gene (Co) in apple (Malus domestica) was explored based on the population of 105 progenies of 'Spur Fuji' x 'Telamon' by bulk segregant analysis (BSA) and Random Amplified Polymorphic DNA (RAPD) technique. A total of 300 random primers were screened and a RAPD marker (S1142(682)) closely linked to Co gene (2.86 cM) was identified and then sequenced.

[Effects of long-term fertilization on wheat yield on Loess Plateau].

The effect of long-term fertilization on the yield of winter wheat was studied on the basis of 18-year located experiment on the Loess Plateau. The results showed that the winter wheat yield increased 418.1 kg.