PGC7 maintains the pluripotency of F9 embryonic carcinoma cells by promoting Nanog translation.

Primordial germ cell 7 (PGC7) is prominently expressed in primordial germ cells (PGCs) and embryonic stem cells (ESCs), serving as a pivotal marker for discerning stem cell pluripotency. However, the role of PGC7 in regulating core pluripotency factors remains unclear. In this study, the expression dynamics of PGC7 and pluripotency- associated proteins are systematically evaluated by quantitative reverse transcription PCR (RT-qPCR) and western blot analysis.

Plant-Plant Interactions Drive the Decomposition of Soil Organic Carbon via Nutrition Competition in Dryland.

Plant-plant interactions are often overlooked when assessing carbon (C) cycling in plant community. Limited research exists on how nutrient competition influences soil organic carbon (SOC) dynamics via modifying rhizosphere C turnover. To address this issue, quantitative model of plant-plant interactions was established in three intercropping systems across 4 years.

Microplastic-Induced Alterations in Soil Aggregate-Associated Carbon Stabilization Pathways: Evidence from δC Signature Analysis.

Microplastics (MPs) are known to affect soil carbon stability in a numerous ways. However, the mechanisms by which they alter the carbon stability within soil aggregates remain unclear . Herein, a one-year field experiment was conducted in an arid agricultural region employing stable isotope techniques to evaluate the soil organic carbon flow in the presence of both persistent (PE, PVC) and biodegradable (PLA, PHA) MPs.

Functional diversity of two apple paralogs MADS5 and MADS35 in regulating flowering and parthenocarpy.

MADS-box genes play important roles in plant development, especially flowering and fruiting. In this study, we identified 54 type I and 69 type II MADS-box genes from the apple reference genome 'GDDH13'. The type II MADS-box genes were further divided into 12 closely related subgroups, each exhibiting similar gene structures and conserved domains.

Protocol for a randomised controlled feasibility trial of an integrated psychosexual intervention for sexual difficulties in people with Multiple Sclerosis: the PIMS trial.

Background: Multiple Sclerosis (MS) is a long-term condition that significantly diminishes quality of life (QoL). Sexual difficulties (SDs) are common, but often overlooked symptom, affecting 50-80% of people with MS (PwMS). The emergence of SDs in MS results from the intricate interplay of disease processes, treatments, and psychosocial factors, contributing to a further decline in QoL and adverse effects on body image, relationships, and psychological well-being.

Cellular senescence predicts breast cancer risk from benign breast disease biopsy images.

Background: Each year, millions of women undergo breast biopsies. Of these, 80% are negative for malignancy but some may be at elevated risk of invasive breast cancer (IBC) due to the presence of benign breast disease (BBD). Cellular senescence plays a complex but poorly understood role in breast cancer development and the presence or absence of these cells may have prognostic value.

Spatial and temporal variation of pneumonia incidence among under-five children in central gondar zone, Northwest Ethiopia, 2013- 2022.

Pneumonia is one of the major causes of morbidity and mortality among under-five years old children's worldwide, with Ethiopia having the highest rates among Sub-Saharan African nations. Effective control and preventative measures will be made clear by comprehending the spatial, temporal, and spatiotemporal variation of pneumonia incidence among under-five children. A time series cross-sectional study design was conducted from 1 January 2013 to 31 December 2022 using pneumonia reports obtained from the Central Gondar Zone health department and Gondar administrative health department.

Airway-centered invasive pulmonary aspergillosis featuring histopathologic fruiting bodies in an immunocompetent patient with a rare incidental finding of thyroid gland Schwannoma: a case report.

Introduction: Invasive pulmonary aspergillosis (IPA) is typically associated with immunocompromised patients, but rare occurrences have been documented in immunocompetent individuals. This presents diagnostic and therapeutic challenges due to atypical presentations and less apparent risk factors.

Case Presentation: A 67-year-old Cambodian American female with a history of stable pulmonary nodules and a left neck schwannoma presented with a two-week history of cough and hemoptysis.

Utilizing SMOTE-TomekLink and machine learning to construct a predictive model for elderly medical and daily care services demand.

This study aims to construct a prediction model for the demand for medical and daily care services of the elderly and to explore the factors that affect the demand for medical and daily care services of the elderly. In this study, a questionnaire survey on the demand for medical and daily care services of 1291 elderly was conducted using multi-stage stratified whole cluster random sampling. SPSS21.

SVLearn: a dual-reference machine learning approach enables accurate cross-species genotyping of structural variants.

Structural variations (SVs) are diverse forms of genetic alterations and drive a wide range of human diseases. Accurately genotyping SVs, particularly occurring at repetitive genomic regions, from short-read sequencing data remains challenging. Here, we introduce SVLearn, a machine-learning approach for genotyping bi-allelic SVs.

LATS1-modulated ZBTB20 perturbing cartilage matrix homeostasis contributes to early-stage osteoarthritis.

Osteoarthritis (OA) is one of the most common degenerative joint diseases in the elderly, increasing in prevalence and posing a substantial socioeconomic challenge, while no disease-modifying treatments available. Better understanding of the early molecular events will benefit the early-stage diagnosis and clinical therapy. Here, we observed the nucleus accumulation of ZBTB20, a member of ZBTB-protein family, in the chondrocytes of early-stage OA.

Post-Movement Beta Synchronization Induced by Speed Effects IHI from Ipsilateral to Contralateral Motor Cortex.

Beta event-related spectral perturbation (ERSP), including bilateral movement-related beta desynchronization (MRBD) and post-movement beta synchronization (PMBS), can be evoked by unilateral speed movement. A potential correlation might exist between power (de)synchronization and interhemispheric coherence during movement execution. However, during the PMBS phase, the existence of interhemispheric coupling and the effect of speed on it are largely undiscovered.

Molecular surveillance of antifolate drug resistance markers in Plasmodium vivax from Khyber Pakhtunkhwa province, northwest Pakistan.

The emergence and spread of antimalarial drug resistance pose significant challenges in the fight against malaria. Mutations in dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS) in Plasmodium vivax are associated with sulfadoxine-pyrimethamine (SP) drug resistance. This study assessed SP resistance status in P.

Source prevention or end-of-pipe treatment? Green public procurement and corporate environmental investment strategies.

As a market-based environmental policy instrument, Green Public Procurement (GPP) increases environmental legitimacy pressures on companies. However, it is unclear whether and how this change in pressure is reflected in corporate environmental investment strategies. Using manually collected data on corporate environmental investment and green public procurement data obtained through textual analysis techniques, this study observes that GPP significantly promotes corporate environmental investment in source prevention, but has no obvious effect on environmental investment in end-of-pipe treatment, suggesting that enterprises obtaining green procurement orders are more inclined to adopt a preventive environmental investment strategy in response to increasing environmental pressures.

Extracellular ATP receptors P2Ks are involved in regulating systemic calcium of Arabidopsis in response to local NaCl stress.

Extracellular ATP (eATP) and its receptors play important roles in the regulation of the responses of plants to environmental stimulation. In the present work, application of NaCl to the roots of Arabidopsis (Arabidopsis thaliana) seedlings triggered an increase of Ca level not only in the stressed root but also in the systemic organs distant from the root, including the hypocotyl and cotyledon, and the eATP level also was increased in the stressed root and those systemic organs under such local salt stress. Application of exogenous ATP to the root also evoked local and systemic increase of Ca level in the seedlings.

Responses of gut microbiota to altitude in a small mammal on Qinghai-Tibetan Plateau.

Altitude of the plateau may affect the composition and functional diversity of animal gut microbiota. However, the specific effects of altitude on the composition, community structure, and function of the host's gut microbiota, as well as how these effects, through interactions between microbial metabolic products (e.g.

Rhizosphere metabolite dynamics in continuous cropping of vineyards: Impact on microflora diversity and co-occurrence networks.

The metabolism of the crop rhizosphere affects microflora diversity and nutrient cycling. However, understanding rhizosphere metabolism in suitable crops within arid desert environments and its impact on microflora interactions remains limited. Through metagenomic and non-targeted metabolomic sequencing of rhizosphere soils from one uncultivated land and four vineyards with cropping years of 5, 10, 15 and 20 years, the critical importance of rhizosphere metabolites in maintaining bacterial and fungal diversity was elucidated.

Spatial distribution characteristics and influencing factors of milk tea stores in Wuhan based on sDNA and OPGD models.

Milk tea stores have rapidly expanded in Wuhan due to residents' increased consumption demand. Therefore, studying the spatial distribution and influencing factors of stores is important for optimizing their layout and promoting economic development. Using milk-tea store data from Amap City's Point of Interest function and road network data from Baidu HeatMap, we analyzed the spatial distribution characteristics of stores within the third ring road of Wuhan City using ArcGIS.

"Integrated Stacked" Design "Nanobullet" for High Photothermal Conversion in Dual-Mode Lateral Flow Immunoassay.

(), a prevalent foodborne bacterium, necessitates creating sensitive and rapid detection methods for food safety, with lateral flow immunoassays (LFIAs) using nanomaterials as signal tracers being particularly effective. Enhancements in performance and sensitivity are not restricted to the material alone, we propose an "integrated stacked" concept, which combines amorphous active sites, hollow morphology for enhanced reflection, and symmetric structure for strong absorption resonance. This approach leads to significant photothermal enhancement (η = 60.

Endozoochory by the cooperation between beetles and ants in the holoparasitic plant Cynomorium songaricum in the deserts of Northwest China.

Cynomorium songaricum Rupr. first described by Carl Johann (Ivanovič) Ruprecht in 1840 is a desert parasitic plant that mainly parasitizes the roots of Nitraria L. (especially of Nitraria tangutorum Bobrov.

Empty Stomach Sensation and Bothersome Hunger Sensation: Two Relevant Clinical Manifestations in Patients with Dyspepsia.

Background: Dyspepsia, characterized by symptoms in the gastroduodenal region, poses diagnostic challenges despite Rome IV criteria.

Aim: This study aimed to investigate the prevalence of two underreported symptoms, Empty Stomach Sensation and Bothersome Hunger Sensation, in dyspeptic patients, and their association with the disorder of gut-brain interaction.

Methods: A cross-sectional study surveyed 1,211 individuals in northwest Mexico, assessing dyspeptic symptoms with pictograms and a 7-point Likert scale.

CROSS-SPECIES TRANSMISSION OF A GENOGROUP C IN A ZOOLOGICAL COLLECTION IN THE UNITED STATES.

Ferlaviruses have been associated with significant mortality events in squamates. Over a 5-month period in 2012, a mortality event at the Phoenix Zoo involved seven rattlesnakes (), a Sonoran gopher snake (fi), and a Gila monster (). All individuals had been managed within the same group of enclosures at the zoo, and many of the affected individuals had previously been housed together.

AN INVESTIGATION OF AN AMYLOID-LIKE DEPOSITION DISORDER IN REPTILES.

Amyloidosis is the pathologic extracellular deposition of amyloid, a proteinaceous substance, in various tissues and organs. The most common form of amyloidosis in domestic animals is amyloid A amyloidosis, though amyloid light chain, amyloid β, and islet amyloid polypeptide amyloidosis have been documented. In reptiles, amyloidosis, or amyloid-like disorders, are considered rare.

How Leaching of Heavy Metals Changes. Long-Term Environmental Safety of Incineration Fly Ash Solidified by Cement.

The cement industry has achieved the harmless and resourceful disposal of incineration fly ash, but the environmental safety of heavy metals during its service life is still unknown. Therefore, the evolution of heavy metal leaching during the wet-dry cycle of the incineration of fly ash after different solidification treatments has been studied. We provide a theoretical basis for the long-term safe use of incineration fly ash after resource utilization.