Effect of Pravastatin in the Treatment of Patients with Type III Hyperlipoproteinemia.

OBJECTIVE: To determine the efficacy and safety of pravastatin in the treatment of subjects with Type III hyperlipoproteinemia. DESIGN: Randomized, double-blind, placebo-controlled, crossover study. SETTING: Four lipid research clinics in the United States.

Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B.

A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B.

Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay as a candidate reference method for the measurement of apolipoprotein B-100.

A monoclonal antibody-based direct binding enzyme-linked immunosorbent assay (ELISA) for apoprotein (apo) B-100 has been developed for use as a reference method. The assay uses the two well-characterized monoclonal antibodies, MB24 and MB47. MB47, which recognizes an epitope at the low density lipoprotein (LDL) receptor-binding domain of apoB and is specific for apoB-100, is bound to the microtiter plate as the capture antibody.

Measurement of cholesterol, triglycerides, and HDL using compact analysis systems.

The compact analyzers facilitate rapid measurement of cholesterol and other lipid parameters outside the conventional laboratory setting. The current generation of instruments has very different features, operating parameters, and performance characteristics, and they must be selected in accordance with the intended application. All three of the common screening instruments, the DT-60, the Reflotron, and the VISION, have demonstrated the capability to meet current performance guidelines, provided they are operated correctly.

Cholesterol esterification by lecithin-cholesterol acyltransferase in A-I-free plasma.

Lecithin-cholesterol acyltransferase (LCAT) mass, activity and endogenous cholesterol esterification rate were measured in plasma and apolipoprotein A-I-free (A-I-free) plasma from two normolipidemic and two hyperlipidemic subjects, and from a patient with Tangier disease. A-I was removed from plasma by an anti-A-I immunosorbent. LCAT activity was measured using an exogenous substrate.

Interlaboratory proficiency survey of cholesterol and high-density lipoprotein cholesterol measurement.

We conducted a proficiency survey of cholesterol and high-density lipoprotein (HDL) cholesterol analysis in local clinical laboratories to determine whether increased national emphasis on cholesterol measurement had resulted in changes in performance from previous surveys. Sets of frozen aliquots of plasma and HDL supernate pools were sent to nine laboratories for analysis; results were compared with Northwest Lipid Research Center values, and relationships were determined by linear regression. Of all the cholesterol measurements, 81% were considered acceptable (i.